FastQCFastQC Report
Sun 21 May 2023
EGAF00007837356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35323
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT15234.3116383093168755No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG13783.901140899697081No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8812.4941256405175096No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8412.380884975794808No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7772.199699912238485No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7352.080797214279648No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT7102.010021798827959No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7082.004359765591824No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5701.6136794722985026No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5371.5202559239022733No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5111.446649491832517No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4591.2994366276930045No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4571.2937745944568695No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3971.1239135973728167No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3931.1125895309005465No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3851.089941397956006No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3791.0729552982476007No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3741.058800215157263No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3681.0418141154488576No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3641.0304900489765874No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3270.9257424341080882No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2970.8408119355660617No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2890.8181638026215213No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2790.7898536364408459No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2730.7728675367324406No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2670.7558814370240353No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2660.7530504204059677No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2640.7473883871698327No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2590.7332333040794949No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2550.7219092376072248No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2440.6907680548084817No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2310.6539648387736037No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2300.6511338221555361No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2290.6483028055374684No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2230.6313167058290633No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT2170.6143306061206579No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2160.6114995895025904No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2150.6086685728845229No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1990.563372306995442No Hit
ATTACAGACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1940.5492172239051043No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1850.5237380743424964No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1810.5124140078702262No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1810.5124140078702262No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1780.5039209580160235No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1710.48410384168955073No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1670.47277977521728054No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1660.4699487585992129No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1650.4671177419811454No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1650.4671177419811454No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1620.45862469212694273No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1620.45862469212694273No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1500.42465249271013217No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1490.42182147609206466No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1470.4161594428559296No Hit
ATTACAGACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1460.413328426237862No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1390.3935113099113892No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1380.3906802932933216No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1380.3906802932933216No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1280.36237012711264616No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1230.3482150440223084No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1230.3482150440223084No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1220.34538402740424085No Hit
ATTACAGACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1210.3425530107861733No Hit
ATTACAGACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1210.3425530107861733No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1200.3397219941681058No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1200.3397219941681058No Hit
ATTACAGACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.3368909775500382No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1180.33405996093197066No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA1170.3312289443139031No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1140.32273589445970047No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1140.32273589445970047No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1110.31424284460549784No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1100.3114118279874303No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1070.30291877813322765No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1070.30291877813322765No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1010.28593267842482234No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA990.2802706451886872No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA980.2774396285706197No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA960.27177759533448465No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA960.27177759533448465No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA950.26894657871641703No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA940.2661155620983495No Hit
ATTACAGACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT930.26328454548028196No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG910.25762251224414684No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG900.25479149562607933No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT900.25479149562607933No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT890.2519604790080118No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT890.2519604790080118No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT850.24063641253574156No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA840.23780539591767405No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA810.22931234606347137No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT780.22081929620926874No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT770.21798827959120118No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA760.21515726297313365No Hit
ATTACAGACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT760.21515726297313365No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC760.21515726297313365No Hit
ATTACAGACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT740.20949522973699855No Hit
ATTACAGACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT740.20949522973699855No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA720.20383319650086346No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA720.20383319650086346No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC710.2010021798827959No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC710.2010021798827959No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.2010021798827959No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA700.19817116326472836No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA700.19817116326472836No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA690.1953401466466608No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG690.1953401466466608No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA670.18967811341052573No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC660.18684709679245817No Hit
ATTACAGACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT660.18684709679245817No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC660.18684709679245817No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA650.18401608017439064No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC640.18118506355632308No Hit
ATTACAGACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT630.17835404693825552No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA630.17835404693825552No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.17835404693825552No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC620.17552303032018798No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA610.17269201370212042No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA600.1698609970840529No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA590.16702998046598533No Hit
ATTACAGACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT580.1641989638479178No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA580.1641989638479178No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.16136794722985023No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA570.16136794722985023No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.1585369306117827No Hit
ATTACAGACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT550.15570591399371514No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT550.15570591399371514No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA550.15570591399371514No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT540.1528748973756476No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT520.1472128641395125No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.14438184752144495No Hit
ATTACAGACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT510.14438184752144495No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA500.14155083090337742No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCT490.13871981428530986No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT480.13588879766724232No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC480.13588879766724232No Hit
ATTACAGACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT480.13588879766724232No Hit
ATTACAGACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT480.13588879766724232No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA470.13305778104917476No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT460.1302267644311072No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT460.1302267644311072No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC460.1302267644311072No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC460.1302267644311072No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC450.12739574781303967No Hit
ATTACAGACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT450.12739574781303967No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT450.12739574781303967No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCT440.12456473119497212No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA430.12173371457690457No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC430.12173371457690457No Hit
ATTACAGACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT430.12173371457690457No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT420.11890269795883703No Hit
ATTACAGACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT420.11890269795883703No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT420.11890269795883703No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA420.11890269795883703No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA400.11324066472270192No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA400.11324066472270192No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC390.11040964810463437No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC390.11040964810463437No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCT380.10757863148656682No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA380.10757863148656682No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA370.10474761486849928No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA370.10474761486849928No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT360.10191659825043173No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA360.10191659825043173No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG360.10191659825043173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)