FastQCFastQC Report
Sun 21 May 2023
EGAF00007837366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70930
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA23403.2990272099252786No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA21673.055124770900888No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG21373.0128295502608204No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG20262.85633723389257No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT14151.9949245735231917No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT12341.7397434089947836No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11251.5860707740025375No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10931.5409558719864656No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT10721.5113492175384182No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG10671.5043000140984069No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9831.3858733963062173No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9641.3590864232341746No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9461.333709290850134No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9391.3238404060341182No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT9201.2970534329620753No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8771.2364302833779783No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8681.223741717185958No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8631.2166925137459468No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA8551.2054137882419287No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8461.1927252220499083No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA7951.1208233469617932No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7751.0926265332017482No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7391.041872268433667No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7171.0108557732976173No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6920.975609756097561No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA6680.9417735795855068No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6390.9008881996334415No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6350.8952488368814324No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5930.8360355279853376No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5820.8205272804173128No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5680.8007895107852813No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT5670.799379670097279No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5620.7923304666572678No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5400.761313971521218No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4920.6936416184971098No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4900.6908219371211053No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4790.6753136895530805No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4780.6739038488650783No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT4730.666854645425067No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4680.6598054419850558No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4680.6598054419850558No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4490.6330184689130128No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA4400.6203299027209925No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4250.5991822924009587No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4130.5822642041449316No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4090.5766248413929226No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA4070.5738051600169181No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3840.5413788241928662No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3740.5272804173128437No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3680.5188213731848301No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3580.5047229663048076No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3580.5047229663048076No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3480.490624559424785No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3420.48216551529677143No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3190.4497391794727195No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT3180.4483293387847173No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3150.4440998167207106No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3120.43987029465670385No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3060.4314112505286903No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3030.42718172846468344No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2990.42154236571267445No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2830.39898491470463837No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2830.39898491470463837No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2810.3961652333286339No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2800.3947553926406316No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2770.39052587057662486No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2690.3792471450726068No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2690.3792471450726068No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2670.37642746369660224No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2540.35809953475257295No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2350.3313125616805301No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2310.3256731989285211No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2270.32003383617651204No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2230.31439447342450305No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2210.3115747920484985No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2120.2988862258564782No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2060.2904271817284647No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2000.28196813760045114No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1930.27209925278443536No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1910.26927957140843084No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1880.2650500493444241No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1830.2580008459044128No Hit
TACCTCGTACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1780.2509516424644015No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1760.24813196108839702No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1750.24672212040039476No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1730.24390243902439024No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1700.23967291696038345No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1620.22839419145636541No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1590.22416466939235866No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1570.22134498801635416No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1560.21993514732835193No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1520.2142957845763429No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1430.20160721838432255No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1420.20019737769632032No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1420.20019737769632032No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1400.1973776963203158No Hit
TACCTCGTACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1380.1945580149443113No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1360.19173833356830677No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1360.19173833356830677No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1310.1846891301282955No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1290.181869448752291No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1270.17904976737628647No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT1260.17763992668828424No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1250.17623008600028198No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT1250.17623008600028198No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1240.17482024531227972No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1230.17341040462427745No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1170.16495136049626394No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1170.16495136049626394No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1160.16354151980826165No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1120.15790215705625263No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1110.1564923163682504No Hit
TACCTCGTACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1080.1522627943042436No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1080.1522627943042436No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1070.15085295361624138No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1050.14803327224023685No Hit
TACCTCGTACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.1466234315522346No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1040.1466234315522346No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1030.14521359086423236No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1030.14521359086423236No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1000.14098406880022557No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1000.14098406880022557No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC980.13816438742422107No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCT980.13816438742422107No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA960.13534470604821655No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC920.12970534329620753No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA920.12970534329620753No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA920.12970534329620753No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT920.12970534329620753No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT920.12970534329620753No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT900.126885661920203No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA880.12406598054419851No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC870.12265613985619625No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT860.121246299168194No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT850.11983645848019173No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT850.11983645848019173No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT840.11842661779218949No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT830.11701677710418722No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC810.11419709572818271No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT800.11278725504018047No Hit
TACCTCGTACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT790.1113774143521782No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT790.1113774143521782No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG780.10996757366417596No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA780.10996757366417596No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA770.10855773297617369No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT770.10855773297617369No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA770.10855773297617369No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA760.10714789228817145No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT760.10714789228817145No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG750.10573805160016918No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA740.10432821091216693No Hit
TACCTCGTACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT730.10291837022416467No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT710.10009868884816016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)