FastQCFastQC Report
Sun 21 May 2023
EGAF00007837367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70930
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA22783.2116170872691385No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA20762.9268292682926833No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG18472.603975750740166No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT17522.470040885379952No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG17472.4629916819399407No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT14542.04990836035528No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT12401.748202453122797No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11661.64387424221063No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT10621.4972508106583955No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10231.4422670238263076No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT10091.422529254194276No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9641.3590864232341746No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9581.350627379106161No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8861.2491188495699987No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8621.2152826730579445No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT8401.1842661779218948No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8271.1659382489778654No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA8091.140561116593825No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8051.134921753841816No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7381.0404624277456647No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7351.036232905681658No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA7271.02495418017764No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7191.0136754546736217No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7070.9967573664175947No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA6890.9713802340335541No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6590.9290850133934865No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6440.9079374030734527No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA6340.8938389961934301No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6330.8924291555054279No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT6250.8811504300014098No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA6110.8614126603693782No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5610.7909206259692655No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5260.7415762018891865No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT5260.7415762018891865No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5210.7345269984491752No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4980.7021006626251234No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4960.6992809812491189No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4870.6865924150570986No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4800.6767235302410827No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT4590.6471168757930355No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4470.6301987875370083No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4310.6076413365289722No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT4250.5991822924009587No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4250.5991822924009587No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4170.5879035668969407No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4060.5723953193289159No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA4000.5639362752009023No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3990.5625264345129001No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3880.5470181869448751No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3750.5286902580008459No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3730.5258705766248415No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA3560.501903284928803No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT3490.4920344001127872No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3350.4722966304807557No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3170.44691949809671505No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3130.44128013534470606No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2960.4173128436486677No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2950.4159030029606654No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2950.4159030029606654No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2910.4102636402086564No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2870.40462427745664736No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2790.3933455519526293No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2750.38770618920062033No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2740.38629634851261807No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2700.380656985760609No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2680.37783730438460456No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2640.3721979416325955No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2630.37078810094459325No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2620.369378260256591No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2610.3679684195685888No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2530.35668969406457074No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2460.3468208092485549No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2450.34541096856055264No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2410.33977160580854365No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2240.31580431411250526No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2200.31016495136049627No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2190.308755110672494No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2150.303115747920485No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2030.2861976596644579No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1930.27209925278443536No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1890.2664598900324263No Hit
TACCTCGTACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1880.2650500493444241No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1850.2608205272804173No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1820.25659100521641054No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1690.23826307627238122No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1690.23826307627238122No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1670.2354433948963767No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1660.23403355420837443No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1650.2326237135203722No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT1640.23121387283236997No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1640.23121387283236997No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1570.22134498801635416No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1540.21711546595234738No Hit
TACCTCGTACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1510.21288594388834062No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1490.21006626251233612No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1480.20865642182433386No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1470.2072465811363316No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1450.2044268997603271No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT1390.19596785563231353No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1370.19314817425630904No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1370.19314817425630904No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1340.18891865219230228No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1340.18891865219230228No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1330.18750881150430002No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1320.18609897081629775No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1290.181869448752291No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1240.17482024531227972No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1210.17059072324827296No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1200.16918088256027067No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1190.16777104187226843No Hit
TACCTCGTACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.16636120118426617No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1170.16495136049626394No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1170.16495136049626394No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1170.16495136049626394No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1160.16354151980826165No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.16354151980826165No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1140.16072183843225715No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1120.15790215705625263No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1100.15508247568024813No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1090.15367263499224587No Hit
TACCTCGTACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1080.1522627943042436No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT1060.1494431129282391No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.14803327224023685No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1030.14521359086423236No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1000.14098406880022557No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA980.13816438742422107No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC970.13675454673621878No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT960.13534470604821655No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC920.12970534329620753No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT890.12547582123220075No Hit
TACCTCGTACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT890.12547582123220075No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA880.12406598054419851No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC870.12265613985619625No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC850.11983645848019173No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC850.11983645848019173No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT840.11842661779218949No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC830.11701677710418722No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT830.11701677710418722No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC820.11560693641618498No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA810.11419709572818271No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCT810.11419709572818271No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT800.11278725504018047No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA800.11278725504018047No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA790.1113774143521782No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.10573805160016918No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCT740.10432821091216693No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG740.10432821091216693No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA730.10291837022416467No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT730.10291837022416467No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC720.1015085295361624No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA720.1015085295361624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)