FastQCFastQC Report
Sun 21 May 2023
EGAF00007837375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33080
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14814.477025392986699No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT12163.675937122128174No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9242.79322853688029No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7992.4153567110036276No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7702.327690447400242No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6882.079806529625151No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6762.0435308343409915No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5321.608222490931076No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5271.5931076178960095No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT5041.5235792019347039No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4831.4600967351874243No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT4071.2303506650544134No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC3961.1970979443772674No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3511.0610640870616688No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3391.0247883917775091No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3351.0126964933494558No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3331.0066505441354294No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3300.9975816203143895No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3290.9945586457073761No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3080.9310761789600966No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC3030.9159613059250303No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3010.9099153567110037No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2980.9008464328899637No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2960.8948004836759371No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2750.8313180169286578No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2710.8192261185006047No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT2480.7496977025392987No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2430.7345828295042321No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2360.713422007255139No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2290.6922611850060459No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2280.6892382103990327No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2250.6801692865779928No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2180.6590084643288996No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2030.6136638452237001No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1950.5894800483675937No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1940.5864570737605804No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1910.5773881499395405No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1880.5683192261185006No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1860.562273276904474No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1840.5562273276904474No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1750.5290205562273277No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1570.47460701330108823No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1570.47460701330108823No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1560.471584038694075No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1550.46856106408706166No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1550.46856106408706166No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1510.45646916565900847No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1490.45042321644498184No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1490.45042321644498184No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1450.43833131801692865No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1450.43833131801692865No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1420.42926239419588874No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA1410.4262394195888755No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1390.42019347037484883No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1380.4171704957678356No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1320.3990326481257557No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1310.39600967351874244No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC1260.38089480048367597No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1190.35973397823458286No Hit
ATTACAGACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1170.35368802902055624No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1140.34461910519951633No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1110.3355501813784764No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1110.3355501813784764No Hit
ATTACAGACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1100.3325272067714631No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1100.3325272067714631No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1090.3295042321644498No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1090.3295042321644498No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.3264812575574365No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1080.3264812575574365No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1080.3264812575574365No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1060.3204353083434099No Hit
ATTACAGACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1040.3143893591293833No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1040.3143893591293833No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1010.3053204353083434No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT980.2962515114873035No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC970.2932285368802902No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC960.29020556227327693No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA960.29020556227327693No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT960.29020556227327693No Hit
ATTACAGACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT930.281136638452237No Hit
ATTACAGACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT920.2781136638452237No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA900.2720677146311971No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC880.2660217654171705No Hit
ATTACAGACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT880.2660217654171705No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT860.25997581620314386No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT860.25997581620314386No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT840.2539298669891173No Hit
ATTACAGACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT840.2539298669891173No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC830.25090689238210395No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA820.2478839177750907No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC810.2448609431680774No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA810.2448609431680774No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT810.2448609431680774No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT800.24183796856106407No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA800.24183796856106407No Hit
ATTACAGACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT800.24183796856106407No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC780.2357920193470375No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT770.23276904474002416No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA770.23276904474002416No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA760.22974607013301088No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA760.22974607013301088No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA750.2267230955259976No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA750.2267230955259976No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.22370012091898428No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT710.21463119709794437No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC700.21160822249093109No Hit
ATTACAGACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT690.2085852478839178No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA690.2085852478839178No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT670.20253929866989118No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT660.19951632406287784No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA640.19347037484885127No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT640.19347037484885127No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA640.19347037484885127No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA640.19347037484885127No Hit
ATTACAGACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT610.18440145102781139No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC610.18440145102781139No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA600.18137847642079807No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC600.18137847642079807No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG580.17533252720677148No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA580.17533252720677148No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT580.17533252720677148No Hit
ATTACAGACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT580.17533252720677148No Hit
ATTACAGACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT570.17230955259975816No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA540.16324062877871826No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT530.16021765417170494No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA520.15719467956469166No Hit
ATTACAGACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT510.15417170495767837No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.15417170495767837No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA510.15417170495767837No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA510.15417170495767837No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT510.15417170495767837No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC510.15417170495767837No Hit
ATTACAGACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT500.15114873035066506No Hit
ATTACAGACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT500.15114873035066506No Hit
ATTACAGACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT500.15114873035066506No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT490.14812575574365175No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA490.14812575574365175No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA490.14812575574365175No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA490.14812575574365175No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG480.14510278113663846No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC480.14510278113663846No Hit
ATTACAGACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT480.14510278113663846No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC480.14510278113663846No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA470.14207980652962515No Hit
ATTACAGACCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC460.13905683192261184No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC450.13603385731559856No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT450.13603385731559856No Hit
ATTACAGACCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT450.13603385731559856No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA450.13603385731559856No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA410.12394195888754535No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT400.12091898428053204No Hit
ATTACAGACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT400.12091898428053204No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT400.12091898428053204No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA390.11789600967351875No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC380.11487303506650544No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT380.11487303506650544No Hit
ATTACAGACCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC380.11487303506650544No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC370.11185006045949214No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTG370.11185006045949214No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT370.11185006045949214No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACC360.10882708585247886No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG360.10882708585247886No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA350.10580411124546554No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT350.10580411124546554No Hit
ATTACAGACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT350.10580411124546554No Hit
ATTACAGACCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT350.10580411124546554No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT350.10580411124546554No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG340.10278113663845223No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT340.10278113663845223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)