FastQCFastQC Report
Sun 21 May 2023
EGAF00007837379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44313
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT29056.555638300273057No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA27026.0975334551937355No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA23985.4115045246315985No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA13533.0532800758242504No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT13282.996863222981969No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA12062.7215489811116376No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7591.7128156522916524No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7221.6293187100850766No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6661.502944959718367No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6641.4984316114909846No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6331.4284747139665561No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6041.3630311646695101No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5501.241170762530183No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5401.2186040213932705No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5371.211833999052197No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4991.1260803827319297No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4491.0132466770473676No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4220.9523164759777041No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3950.8913862749080406No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3860.8710762078848194No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3610.8146593550425383No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT3400.7672691986550222No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT3370.7604991763139485No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3240.7311624128359623No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3240.7311624128359623No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3100.699568975244285No Hit
ATTACAGACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3040.6860289305621375No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2950.6657188635389164No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2890.6521788188567689No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2880.6499221447430776No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2710.6115586848103266No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC2640.5957619660144878No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2580.5822219213323404No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2470.5573985060817368No Hit
ATTACAGACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2450.5528851578543542No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2400.541601787285898No Hit
ATTACAGACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2400.541601787285898No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2200.49646830501207323No Hit
ATTACAGACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2140.48292826032992575No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2140.48292826032992575No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2100.4739015638751608No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2050.46261819330670456No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2010.4535914968519396No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1960.4423081262834834No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1960.4423081262834834No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1930.4355381039424097No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1890.42651140748764477No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT1880.4242547333739534No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1830.4129713628054973No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1800.4062013404644235No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1760.3971746440096586No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT1710.3858912734412024No Hit
ATTACAGACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1670.3768645769864374No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1620.36558120641798114No Hit
ATTACAGACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1590.3588111840769075No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1510.34075779116737753No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1490.33624444293999506No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1490.33624444293999506No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1480.33398776882630377No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1470.33173109471261253No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1460.3294744205989213No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA1430.3227043982578476No Hit
ATTACAGACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1400.31593437591677387No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1370.30916435357570016No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1350.3046510053483176No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1280.28885428655247897No Hit
ATTACAGACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.2843409383250965No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1230.2775709159840227No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1230.2775709159840227No Hit
ATTACAGACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.2753142418703315No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1210.27305756775664025No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC1180.26628754541556654No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1170.2640308713018753No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT1170.2640308713018753No Hit
ATTACAGACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.2640308713018753No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1160.26177419718818407No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1150.25951752307449283No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1100.24823415250603661No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1090.24597747839234535No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1080.24372080427865414No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1000.2256674113691242No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA960.2166407149143592No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT940.2121273666869767No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA940.2121273666869767No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA930.2098706925732855No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC920.20761401845959424No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT910.20535734434590303No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA900.20310067023221176No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA880.1985873220048293No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC860.19407397377744678No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA860.19407397377744678No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA860.19407397377744678No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC860.19407397377744678No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT860.19407397377744678No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA850.19181729966375555No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA850.19181729966375555No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT840.1895606255500643No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC820.18504727732268184No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT820.18504727732268184No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.18053392909529933No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA800.18053392909529933No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.17602058086791686No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA770.17376390675422562No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC750.16925055852684312No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT740.16699388441315188No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.16473721029946065No Hit
ATTACAGACCCGGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCT720.1624805361857694No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA710.16022386207207817No Hit
ATTACAGACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT690.1557105138446957No Hit
ATTACAGACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT680.15345383973100446No Hit
ATTACAGACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT670.1511971656173132No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT670.1511971656173132No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC670.1511971656173132No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC640.14442714327623948No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA620.139913795048857No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA600.1354004468214745No Hit
ATTACAGACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT600.1354004468214745No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC590.13314377270778327No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA590.13314377270778327No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC590.13314377270778327No Hit
ATTACAGACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT590.13314377270778327No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT580.13088709859409203No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT570.1286304244804008No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT570.1286304244804008No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC570.1286304244804008No Hit
ATTACAGACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT560.12637375036670956No Hit
ATTACAGACCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.12637375036670956No Hit
ATTACAGACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT560.12637375036670956No Hit
ATTACAGACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC540.12186040213932707No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.12186040213932707No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT540.12186040213932707No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC540.12186040213932707No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG530.1196037280256358No Hit
ATTACAGACCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT530.1196037280256358No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.11734705391194457No Hit
ATTACAGACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT520.11734705391194457No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT510.11509037979825333No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA510.11509037979825333No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACC510.11509037979825333No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.11509037979825333No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA510.11509037979825333No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCGCACC510.11509037979825333No Hit
ATTACAGACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT510.11509037979825333No Hit
ATTACAGACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT510.11509037979825333No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA510.11509037979825333No Hit
ATTACAGACCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT500.1128337056845621No Hit
ATTACAGACCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT480.1083203574571796No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT470.10606368334348835No Hit
ATTACAGACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.10606368334348835No Hit
ATTACAGACCCGGACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCT460.10380700922979712No Hit
ATTACAGACCCGGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCT460.10380700922979712No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA450.10155033511610588No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG450.10155033511610588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)