FastQCFastQC Report
Sun 21 May 2023
EGAF00007837442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35247
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT14544.125173773654495No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12603.5747723210486No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11653.305245836525094No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9742.7633557465883625No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7322.0767724912758534No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6951.9717990183561722No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT6831.9377535676795188No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6831.9377535676795188No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6541.8554770618776066No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5581.58311345646438No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4691.330609697279201No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4671.324935455499759No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4591.30223848838199No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4461.2653559168156157No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4331.2284733452492411No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4281.2142877408006356No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3881.1008029052117911No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3821.0837801798734643No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3671.0412233665276478No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3631.0298748829687632No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3490.9901551905126679No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3480.9873180696229467No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3180.9022044429313134No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3170.8993673220415922No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC3130.8880188384827077No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3050.8653218713649389No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA3000.8511362669163334No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2850.8085794535705166No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2790.79155672823219No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2690.7631855193349788No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2640.7489999148863733No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2570.7291400686583256No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2530.7177915850994411No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2310.6553749255255766No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2310.6553749255255766No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2200.6241665957386444No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2160.61281811217976No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2100.5957953868414333No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2090.5929582659517122No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2060.5844469032825489No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2050.5816097823928277No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2050.5816097823928277No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1990.5645870570545011No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1920.5447272108264534No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1910.5418900899367322No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1870.5305416063778478No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1860.5277044854881267No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1830.5191931228189633No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1710.48514767214230997No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1700.4823105512525888No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1660.4709620676937044No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1650.46812494680398326No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1640.4652878259142622No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1510.42840525434788773No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1480.41989389167872443No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1460.41421964989928217No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1440.40854540811984No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1430.40570828723011887No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1410.4000340454506766No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1400.39719692456095557No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1380.3915226827815133No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1380.3915226827815133No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1360.3858484410020711No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1340.3801741992226289No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1340.3801741992226289No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1250.3546401112151389No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1240.3518029903254178No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1210.34329162765625443No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1190.3376173858768122No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1180.3347802649870911No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1180.3347802649870911No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1160.32910602320764887No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1140.32343178142820667No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1130.32059466053848557No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1100.3120832978693222No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1040.29506057253099555No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1040.29506057253099555No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1030.29222345164127445No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1030.29222345164127445No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1010.2865492098618322No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1010.2865492098618322No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG980.2780378471926689No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA980.2780378471926689No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG980.2780378471926689No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA970.2752007263029478No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA950.2695264845235055No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT920.2610151218543422No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT910.2581780009646211No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT860.24399239651601554No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT830.2354810338468522No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC790.22413255028796777No Hit
ATTCCTTCACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT780.22129542939824665No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT760.21562118761880444No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA760.21562118761880444No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT750.21278406672908334No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA750.21278406672908334No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT740.20994694583936221No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC720.20427270405992No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT700.19859846228047778No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC700.19859846228047778No Hit
ATTCCTTCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT680.19292422050103555No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA680.19292422050103555No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA670.19008709961131445No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA670.19008709961131445No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT670.19008709961131445No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA650.18441285783187222No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCT630.17873861605243No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA620.1759014951627089No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.1759014951627089No Hit
ATTCCTTCACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT610.17306437427298776No Hit
ATTCCTTCACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT590.16739013249354556No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT580.16455301160382443No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG570.16171589071410333No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT570.16171589071410333No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC570.16171589071410333No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA570.16171589071410333No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT560.1588787698243822No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT540.15320452804494No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA540.15320452804494No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG540.15320452804494No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA540.15320452804494No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC520.14753028626549777No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.14753028626549777No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC510.14469316537577667No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA510.14469316537577667No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC490.13901892359633444No Hit
ATTCCTTCACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT490.13901892359633444No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC480.13618180270661334No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCGCACC480.13618180270661334No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT480.13618180270661334No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA480.13618180270661334No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA470.1333446818168922No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.1305075609271711No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA460.1305075609271711No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA450.12767044003744998No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA430.12199619825800777No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT430.12199619825800777No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA420.11915907736828667No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT420.11915907736828667No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT420.11915907736828667No Hit
ATTCCTTCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT420.11915907736828667No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC420.11915907736828667No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA420.11915907736828667No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT420.11915907736828667No Hit
ATTCCTTCACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT410.11632195647856555No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT400.11348483558884442No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA400.11348483558884442No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC390.11064771469912332No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT390.11064771469912332No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCT390.11064771469912332No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA380.10781059380940222No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG380.10781059380940222No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT380.10781059380940222No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT370.10497347291968111No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA370.10497347291968111No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA370.10497347291968111No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC370.10497347291968111No Hit
ATTCCTTCACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT360.10213635202996No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA360.10213635202996No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA360.10213635202996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)