FastQCFastQC Report
Sun 21 May 2023
EGAF00007837472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64446
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT24873.8590447816776834No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA24673.828011048009186No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA23263.6092232256462777No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA20563.190267821121559No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA19583.0382025261459207No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG18952.940446265090153No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG17092.651832541973125No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT16882.619247121621202No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT11971.8573689600595849No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT9971.5470316233746082No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9741.511342829655836No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9411.4601371691028149No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA9401.45858548241939No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9151.4197933153337678No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG9131.4166899419669179No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9041.402724761816094No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT8041.2475560934736059No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7781.2072122397045588No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7511.1653166992520871No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7161.1110076653322163No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7151.1094559786487912No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6310.979114297241101No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6010.9325636967383546No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5880.9123917698538311No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG5180.8037737020140894No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5140.7975669552803898No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5060.7851534618129907No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4900.7603264748781926No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC4710.7308444278931199No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4670.7246376811594203No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT4530.702914067591472No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4530.702914067591472No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4400.6827421407069484No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA4140.6423982869379015No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC4130.6408466002544766No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT4010.6222263600533781No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA4010.6222263600533781No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3670.5694690128169321No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3580.5555038326661081No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT3280.5089532321633615No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT3210.49809142537938744No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3180.4934363653291128No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3160.49033299196226293No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3080.47791949849486387No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2980.46240263166061507No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2970.4608509449771902No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2970.4608509449771902No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2940.45619588492691554No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA2870.44533407814294135No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2860.4437823914595165No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2790.4329205846755423No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2780.4313688979921174No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA2760.4282655246252677No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2440.37861155075567143No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2400.3724048040219719No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2400.3724048040219719No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA2380.3693014306551221No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT2320.3599913105545728No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2320.3599913105545728No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2310.35843962387114797No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA2270.35223287713744844No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2250.34912950377059865No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2240.3475778170871737No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2240.3475778170871737No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2190.3398193836700493No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2140.3320609502529249No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC2100.3258542035192254No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2100.3258542035192254No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2040.3165440834186761No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1940.30102721658442727No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC1930.2994755299010024No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1890.2932687831673028No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1860.2886137231170282No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1820.2824069763833287No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1760.27309685628277935No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1710.26533842286565495No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1670.2591316761319554No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1660.25757998944853056No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1640.25447661608168076No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1580.24516649598113147No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1570.24361480929770662No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1510.2343046891971573No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1470.22809794246345777No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1430.22189119572975824No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1430.22189119572975824No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1420.22033950904633337No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCT1380.21413276231263384No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1360.21102938894578407No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1350.20947770226235918No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1330.2063743288955094No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1310.20327095552865965No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1310.20327095552865965No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT1310.20327095552865965No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1300.20171926884523478No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1270.19706420879496012No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1260.19551252211153525No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1220.18930577537783572No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1210.18775408869441082No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1190.18465071532756105No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1190.18465071532756105No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1180.18309902864413619No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1180.18309902864413619No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1160.1799956552772864No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT1160.1799956552772864No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.1799956552772864No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1140.17689228191043663No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1130.17534059522701176No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1120.17378890854358686No Hit
ATTCCTTCACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1060.1644787884430376No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.1629271017596127No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1050.1629271017596127No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1020.15827204170933806No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT1020.15827204170933806No Hit
ATTCCTTCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1020.15827204170933806No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1010.15672035502591317No Hit
ATTCCTTCACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.1551686683424883No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT1000.1551686683424883No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC1000.1551686683424883No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC980.15206529497563853No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG950.14741023492536387No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA950.14741023492536387No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA930.1443068615585141No Hit
ATTCCTTCACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT920.14275517487508924No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC910.14120348819166434No Hit
ATTCCTTCACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT870.1349967414579648No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA870.1349967414579648No Hit
ATTCCTTCACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT860.1334450547745399No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT840.13034168140769015No Hit
ATTCCTTCACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT830.12878999472426528No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCT820.12723830804084038No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT810.1256866213574155No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA810.1256866213574155No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC810.1256866213574155No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC800.12413493467399062No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT800.12413493467399062No Hit
ATTCCTTCACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT800.12413493467399062No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC790.12258324799056573No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC790.12258324799056573No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCT780.12103156130714085No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA780.12103156130714085No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA760.11792818794029108No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA760.11792818794029108No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT750.11637650125686623No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA750.11637650125686623No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG750.11637650125686623No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA740.11482481457344135No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA740.11482481457344135No Hit
ATTCCTTCACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT740.11482481457344135No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC740.11482481457344135No Hit
ATTCCTTCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT710.11016975452316669No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.11016975452316669No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCT710.11016975452316669No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT700.1086180678397418No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT700.1086180678397418No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA700.1086180678397418No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA700.1086180678397418No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT690.10706638115631692No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT690.10706638115631692No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC680.10551469447289204No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT680.10551469447289204No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA650.10085963442261739No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)