FastQCFastQC Report
Sun 21 May 2023
EGAF00007837473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64446
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT31714.920398473140303No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA21403.3206095025292495No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA19613.0428575861961953No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA19453.018030599261397No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA18462.864413617602334No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT17512.7170033826769697No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG16732.595971821369829No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG15682.4330447196102165No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT13442.0854669025230423No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT12741.9768488346833006No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9431.4632405424696646No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8971.3918629550321198No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8721.3530707879464978No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8431.3080718741271762No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8421.3065201874437513No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8171.2677280203581294No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7941.232039226639357No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7861.219625733171958No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7681.1916953728703101No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT7311.1342829655835893No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5910.9170468299041057No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT5770.8953232163361574No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5760.8937715296527325No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5690.8829097228687584No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5460.847220929149986No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5360.8317040623157372No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5350.8301523756323124No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC5160.8006703286472395No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5090.7898085218632654No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG4800.7448096080439438No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4560.7075691276417465No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4540.7044657542748969No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA4130.6408466002544766No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC4030.6253297334202278No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3980.6175713000031033No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3820.5927443130683052No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3560.5524004592992583No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3560.5524004592992583No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3440.5337802190981596No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3340.5182633522639108No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3280.5089532321633615No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT3240.502746485429662No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3200.49653973869596246No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT3150.48878130527883806No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3110.48257455854513853No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT3040.47171275176116434No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3000.4655060050274649No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2930.45464419824349067No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA2920.4530925115600658No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2900.449989138193216No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2790.4329205846755423No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA2750.42671383794184276No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2680.4158520311578686No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2680.4158520311578686No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2500.3879216708562207No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA2450.3801632374390963No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2450.3801632374390963No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2450.3801632374390963No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2400.3724048040219719No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2320.3599913105545728No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA2250.34912950377059865No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2180.33826769698662446No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2140.3320609502529249No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT2130.33050926356950006No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC2130.33050926356950006No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2120.3289575768860752No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT2110.32740589020265026No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2040.3165440834186761No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1870.2901654098004531No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1860.2886137231170282No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1830.28395866306675355No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1760.27309685628277935No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1730.26844179623250475No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1720.2668901095490798No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1630.2529249293982559No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1590.2467181826645564No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1560.2420631226142817No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1480.2296496291468827No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1480.2296496291468827No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1470.22809794246345777No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1440.22344288241318314No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1430.22189119572975824No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1430.22189119572975824No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1420.22033950904633337No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1420.22033950904633337No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1420.22033950904633337No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1420.22033950904633337No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC1410.21878782236290847No Hit
ATTCCTTCACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1390.2156844489960587No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1390.2156844489960587No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1380.21413276231263384No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1380.21413276231263384No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1360.21102938894578407No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1350.20947770226235918No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1340.2079260155789343No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1340.2079260155789343No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT1330.2063743288955094No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1310.20327095552865965No Hit
ATTCCTTCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1300.20171926884523478No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCT1290.20016758216180988No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1290.20016758216180988No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1280.198615895478385No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1270.19706420879496012No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1260.19551252211153525No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1260.19551252211153525No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1210.18775408869441082No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.1799956552772864No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1150.17844396859386152No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1100.1706855351767371No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1090.16913384849331223No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT1050.1629271017596127No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1050.1629271017596127No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1050.1629271017596127No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1040.16137541507618783No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1040.16137541507618783No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1040.16137541507618783No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1040.16137541507618783No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA1040.16137541507618783No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1030.15982372839276293No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC1000.1551686683424883No Hit
ATTCCTTCACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1000.1551686683424883No Hit
ATTCCTTCACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT990.1536169816590634No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT980.15206529497563853No Hit
ATTCCTTCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT960.14896192160878877No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT960.14896192160878877No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA940.145858548241939No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT940.145858548241939No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG910.14120348819166434No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCT890.13810011482481457No Hit
ATTCCTTCACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT880.13654842814138968No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA850.13189336809111504No Hit
ATTCCTTCACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT840.13034168140769015No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA830.12878999472426528No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA830.12878999472426528No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA820.12723830804084038No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC800.12413493467399062No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG790.12258324799056573No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC790.12258324799056573No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA780.12103156130714085No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA740.11482481457344135No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC740.11482481457344135No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCT730.11327312789001645No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA730.11327312789001645No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG720.11172144120659157No Hit
ATTCCTTCACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT710.11016975452316669No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT700.1086180678397418No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC680.10551469447289204No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA680.10551469447289204No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA660.10241132110604227No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC660.10241132110604227No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT660.10241132110604227No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)