FastQCFastQC Report
Sun 21 May 2023
EGAF00007837486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35251
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16824.77149584408953No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13673.8779041729312644No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT12143.443873932654393No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9702.751694987376245No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7172.0339848514935746No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6791.9261864911633713No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6231.7673257496241241No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5971.6935689767666167No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT5881.6680377861620947No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5841.6566905903378628No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4661.3219483135230206No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4541.2879067260503247No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4421.2538651385776292No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4391.245354741709455No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4251.2056395563246434No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4241.2028027573685853No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4161.1801083657201215No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4041.1460667782474256No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4001.1347195824231937No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3981.1290459845110778No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT3971.1262091855550196No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3771.0694732064338601No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3621.0269212220929902No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2960.8396924909931633No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT2950.8368556920371053No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2880.8169980993446995No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2690.7630989191795977No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2640.7489149243993078No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2520.714873336926612No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2490.7063629400584381No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2410.6836685484099742No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC2360.6694845536296843No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2210.6269325692888146No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA2140.6070749765964086No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2100.5957277807721767No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2060.5843805849479448No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2040.5787069870358288No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT1910.5418286006070749No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1880.533318203738901No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1870.530481404782843No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1840.5219710079146691No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1830.5191342089586111No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1820.5162974100025531No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1780.5049502141783212No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1750.49643981731014725No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1670.4737454256616833No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1650.46807182774956735No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1600.4538878329692774No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1550.43970383818898756No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1510.4283566423647556No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1480.41984624549658167No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1460.4141726475844657No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1460.4141726475844657No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1440.40849904967234973No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1390.39431505489205976No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1370.38864145697994384No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1360.38580465802388586No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1350.3829678590678279No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1330.37729426115571185No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1290.36594706533147997No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1280.363110266375422No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1270.360273467419364No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1240.35176307055119005No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1210.34325267368301604No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1190.3375790757709001No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1170.33190547785878416No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1140.3233950809906102No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1130.3205582820345522No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1130.3205582820345522No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1120.31772148307849424No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1090.30921108621032023No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1090.30921108621032023No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1070.3035374882982043No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1060.30070068934214633No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC1010.2865166945618564No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1000.2836798956057984No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG980.27800629769368246No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT960.27233269978156643No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT940.2666591018694505No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA930.26382230291339254No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA930.26382230291339254No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT920.26098550395733455No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.26098550395733455No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT910.25814870500127657No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT870.24680150917704463No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA860.24396471022098665No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT850.24112791126492866No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC850.24112791126492866No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT840.23829111230887068No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT840.23829111230887068No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT830.23545431335281267No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA830.23545431335281267No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT820.23261751439675468No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC800.2269439164846387No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT780.22127031857252277No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT780.22127031857252277No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA760.2155967206604068No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.21275992170434882No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCT750.21275992170434882No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA730.20708632379223285No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA720.20424952483617487No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA700.19857592692405887No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT700.19857592692405887No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC690.1957391279680009No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC670.19006553005588492No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA660.18722873109982696No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT650.18439193214376898No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC640.181555133187711No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA640.181555133187711No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA640.181555133187711No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA630.178718334231653No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT620.17588153527559502No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT610.173044736319537No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.173044736319537No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT610.173044736319537No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT610.173044736319537No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA600.17020793736347906No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCT590.16737113840742107No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA590.16737113840742107No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT580.1645343394513631No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC580.1645343394513631No Hit
ATTCCTTCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT560.15886074153924712No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT560.15886074153924712No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCT560.15886074153924712No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT550.15602394258318913No Hit
ATTCCTTCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT540.15318714362713115No Hit
ATTCCTTCACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT540.15318714362713115No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA530.15035034467107317No Hit
ATTCCTTCACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT530.15035034467107317No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.14751354571501518No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT520.14751354571501518No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA520.14751354571501518No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC510.1446767467589572No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT500.1418399478028992No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG490.13900314884684123No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCT490.13900314884684123No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACC490.13900314884684123No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC480.13616634989078322No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA480.13616634989078322No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA460.13049275197866728No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT460.13049275197866728No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC440.12481915406655131No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC440.12481915406655131No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA430.12198235511049332No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT430.12198235511049332No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT420.11914555615443534No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT420.11914555615443534No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC410.11630875719837734No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA410.11630875719837734No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC410.11630875719837734No Hit
ATTCCTTCACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT410.11630875719837734No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT400.11347195824231936No Hit
ATTGGACAGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG400.11347195824231936No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT380.1077983603302034No Hit
ATTGGACAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA370.10496156137414542No Hit
ATTGGACAGGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG370.10496156137414542No Hit
ATTCCTTCACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT360.10212476241808743No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT360.10212476241808743No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCT360.10212476241808743No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT360.10212476241808743No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC360.10212476241808743No Hit
ATTGGACAGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA360.10212476241808743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)