FastQCFastQC Report
Sun 21 May 2023
EGAF00007837517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63737
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT26314.127900591493168No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT18842.9558968887773194No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT17732.781743728132796No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA15372.411472143339034No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA14332.2483016144468673No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13802.1651474026075905No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA13582.1306305599573245No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT11021.7289800272996845No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA10301.6160158149897235No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA10091.5830679197326514No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA8951.404207916908546No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8601.3492947581467594No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8511.3351742316080142No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA7951.2473131775891555No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCT7831.2284858088708286No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7561.186124229254593No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7411.1625900183566846No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT7371.156314228783909No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7181.1265042283132247No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7051.106107912201704No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6951.0904184382697648No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6801.0668842273718564No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6441.0104021212168757No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5550.870765803222618No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5340.8378179079655459No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5310.8331110657859643No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC5130.8048700127084739No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5120.8033010653152799No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA5100.8001631705288922No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5090.7985942231356983No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA5060.7938873809561166No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4780.7499568539466872No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4720.7405431695875238No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4570.7170089586896151No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4470.7013194847576761No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4390.6887679056121249No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT4120.6464063259958894No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4070.6385615890299198No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3980.6244410624911747No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCT3960.6213031677047868No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3940.6181652729183991No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3930.6165963255252052No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT3690.5789415880885513No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT3630.569527903729388No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3590.5632521141566124No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3590.5632521141566124No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3420.5365800084723159No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3060.48009790231733535No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2990.469115270564978No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2960.46440842838539625No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2940.4612705335990085No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2900.4549947440262328No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT2830.4440121122738755No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2830.4440121122738755No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2790.4377363227010998No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2780.4361673753079059No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2770.43459842791471204No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCATGCT2740.4298915857351303No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2690.42204684876916077No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2660.41734000658957904No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2580.40478842744402777No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2550.4000815852644461No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCATGCT2540.3985126378712522No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2420.3796852691529253No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2280.3577200056482106No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2260.3545821108618228No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2230.3498752686822411No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2040.32006526821155684No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2020.316927373425169No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1960.3075136890660056No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1940.3043757942796178No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1920.30123789949323No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1910.2996689521000361No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT1880.2949621099204544No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1860.29182421513406653No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1850.2902552677408726No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1850.2902552677408726No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1820.28554842556129095No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1780.2792726359885153No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1740.2729968464157397No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1730.27142789902254577No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1700.2667210568429641No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1690.26515210944977013No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1680.2635831620565762No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCT1660.26044526727018846No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCATGCT1610.2526005303042189No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCT1580.24789368812463716No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1570.24632474073144325No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1550.24318684594505546No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1530.24004895115866767No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1500.23534210897908592No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1480.23220421419269813No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1450.2274973720131164No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1430.22435947722672858No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1430.22435947722672858No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1420.2227905298335347No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1420.2227905298335347No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1410.2212215824403408No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1360.21337684547437125No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1350.2118078980811773No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1340.21023895068798343No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1320.2071010559015956No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1310.20553210850840173No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCT1300.20396316111520782No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT1280.20082526632881997No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1270.1992563189356261No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1230.19298052936285046No Hit
ATTCCTTCACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1140.1788600028241053No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCATGCT1130.1772910554309114No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1130.1772910554309114No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1110.1741531606445236No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1090.17101526585813578No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCT1080.16944631846494188No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1070.16787737107174797No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCATGCT1040.16317052889216624No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1040.16317052889216624No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1030.16160158149897236No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1030.16160158149897236No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT1030.16160158149897236No Hit
ATTCCTTCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1020.16003263410577842No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGGGCAAGCGTTA1020.16003263410577842No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1010.1584636867125845No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA980.1537568445330028No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT940.1474810549602272No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC940.1474810549602272No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGGGCAAGCGTTA940.1474810549602272No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA910.14277421278064548No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG910.14277421278064548No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACT900.14120526538745154No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT900.14120526538745154No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC900.14120526538745154No Hit
ATTCCTTCACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT900.14120526538745154No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA890.13963631799425766No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG880.13806737060106375No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA860.13492947581467593No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA820.12865368624190032No Hit
ATTCCTTCACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT810.1270847388487064No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC800.1255157914555125No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA800.1255157914555125No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT790.12394684406231858No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA780.12237789666912469No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTATGGGGCGAGCGTTG760.11924000188273687No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCATGCT760.11924000188273687No Hit
ATTCCTTCACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT750.11767105448954296No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA740.11610210709634906No Hit
ATTCCTTCACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT730.11453315970315514No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT730.11453315970315514No Hit
ATTCCTTCACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT730.11453315970315514No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT720.11296421230996125No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT720.11296421230996125No Hit
ATTCCTTCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT720.11296421230996125No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA710.11139526491676735No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC710.11139526491676735No Hit
AAGTTATGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA710.11139526491676735No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT690.10825737013037953No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT680.10668842273718562No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT680.10668842273718562No Hit
ATTCCTTCACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT680.10668842273718562No Hit
AAGTTATGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG660.1035505279507978No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCATGCT650.10198158055760391No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA640.10041263316440999No Hit
AAGTTATGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA640.10041263316440999No Hit
AAGTTATGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA640.10041263316440999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)