FastQCFastQC Report
Sun 21 May 2023
EGAF00007837523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29893
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT12714.251831532465795No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8832.953868798715418No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7982.66952129261031No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7172.398554845616031No Hit
AGGTATCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5841.9536346301809788No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT5391.8030977151841567No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5351.7897166560733282No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT5141.719466095741478No Hit
AGGTATCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4771.5956912989663132No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4741.585655504633192No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4641.5522028568561201No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4491.5020238851905128No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT4461.4919880908573915No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4271.4284280600809556No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC4261.425082795303248No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA3941.3180343224166193No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3911.3079985280834978No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA3741.2511290268624762No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3351.1206637005318971No Hit
AGGTATCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3101.0370320810892182No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3061.0236510219783896No Hit
AGGTATCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3031.0136152276452681No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3011.0069246980898539No Hit
AGGTATCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3001.0035794333121466No Hit
AGGTATCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2860.9567457264242465No Hit
AGGTATCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA2760.9232930786471749No Hit
AGGTATCTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2750.9199478138694678No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2650.8864951660923962No Hit
AGGTATCTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2540.8496972535376176No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT2510.839661459204496No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT2450.819589870538253No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2420.8095540762051316No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2410.8062088114274245No Hit
AGGTATCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA2210.7393035158732814No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCT2190.7326129863178671No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2140.7158866624293313No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT1940.6489813668751881No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1890.6322550429866524No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1890.6322550429866524No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1860.622219248653531No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1790.5988023952095809No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT1790.5988023952095809No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1770.5921118656541665No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1650.5519686883216807No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1630.5452781587662664No Hit
AGGTATCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1510.5051349814337804No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1510.5051349814337804No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1440.4817181279898304No Hit
AGGTATCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1440.4817181279898304No Hit
AGGTATCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1410.471682333656709No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1390.4649918041012946No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1360.4549560097681732No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1330.44492021543505167No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1300.4348844211019302No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1290.43153915632422307No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1220.40812230288027296No Hit
AGGTATCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1210.4047770381025658No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1190.39808650854715155No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1170.39139597899173717No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1170.39139597899173717No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.38805071421403003No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1140.3813601846586157No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1130.3780149198809086No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC1100.3679791255477871No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC1090.36463386077008No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA1060.35459806643695846No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1040.3479075368815442No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT1030.34456227210383705No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1020.34121700732612986No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1020.34121700732612986No Hit
AGGTATCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1020.34121700732612986No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT960.32114541865988694No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA960.32114541865988694No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC930.3111096243267655No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT920.30776435954905834No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT900.301073829993644No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA890.2977285652159368No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA890.2977285652159368No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT860.28769277088281536No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT860.28769277088281536No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT850.28434750610510817No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG830.2776569765496939No Hit
AGGTATCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA820.27431171177198677No Hit
AGGTATCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA810.27096644699427963No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA800.26762118221657244No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT800.26762118221657244No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA780.2609306526611581No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG780.2609306526611581No Hit
AGGTATCTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA780.2609306526611581No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT760.2542401231057438No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT760.2542401231057438No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT750.25089485832803665No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT750.25089485832803665No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT730.24420432877262235No Hit
AGGTATCTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA720.2408590639949152No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT700.23416853443950092No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG680.2274780048840866No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCTAGCT670.2241327401063794No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT650.2174422105509651No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT640.21409694577325797No Hit
AGGTATCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG630.21075168099555078No Hit
AGGTATCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA620.20740641621784364No Hit
AGGTATCTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA600.20071588666242934No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC600.20071588666242934No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT600.20071588666242934No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT600.20071588666242934No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT600.20071588666242934No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC590.19737062188472215No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCC590.19737062188472215No Hit
AGGTATCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA570.19068009232930785No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA570.19068009232930785No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT560.18733482755160072No Hit
AGGTATCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG560.18733482755160072No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC550.18398956277389356No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT540.1806442979961864No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC540.1806442979961864No Hit
AGGTATCTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA540.1806442979961864No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT530.17729903321847923No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT530.17729903321847923No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC510.17060850366306493No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT500.16726323888535777No Hit
AGGTATCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA490.16391797410765063No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT490.16391797410765063No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA480.16057270932994347No Hit
AGGTATCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA470.1572274445522363No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT470.1572274445522363No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT460.15388217977452917No Hit
AGGTATCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG460.15388217977452917No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT450.150536914996822No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT450.150536914996822No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT450.150536914996822No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT450.150536914996822No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT450.150536914996822No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT450.150536914996822No Hit
ATTCCTTCACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT440.14719165021911484No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC440.14719165021911484No Hit
ATTCCTTCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT420.14050112066370052No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA410.13715585588599338No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC410.13715585588599338No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCTAGCT410.13715585588599338No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC400.13381059110828622No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCTAGCT400.13381059110828622No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCT390.13046532633057906No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT380.1271200615528719No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT380.1271200615528719No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT370.12377479677516474No Hit
ATTCCTTCACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT370.12377479677516474No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT360.1204295319974576No Hit
AGGTATCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA360.1204295319974576No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCTAGCT360.1204295319974576No Hit
AGGTATCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA360.1204295319974576No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC350.11708426721975046No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCT350.11708426721975046No Hit
AGGTATCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTA340.1137390024420433No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCT340.1137390024420433No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCT340.1137390024420433No Hit
ATTCCTTCACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT340.1137390024420433No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT330.11039373766433613No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCT330.11039373766433613No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC330.11039373766433613No Hit
AGGTATCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA320.10704847288662898No Hit
ATTCCTTCACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT310.10370320810892182No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT310.10370320810892182No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCT300.10035794333121467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)