FastQCFastQC Report
Sun 21 May 2023
EGAF00007837530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20004
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC6103.049390121975605No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA4712.354529094181164No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT4662.3295340931813637No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4562.2795440911817635No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4022.009598080383923No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3951.9746050789842033No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3861.9296140771845631No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3821.909618076384723No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3731.8646270745850828No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3731.8646270745850828No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3701.849630073985203No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC3551.7746450709858026No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA3451.7246550689862028No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC3141.5696860627874427No Hit
ACGCCTGAGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2901.4497100579884024No Hit
ACGCCTGAGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2741.3697260547890422No Hit
ACGCCTGAGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2731.3647270545890822No Hit
ACGCCTGAGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2721.3597280543891221No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA2531.2647470505898821No Hit
ACGCCTGAGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2451.224755048990202No Hit
ACGCCTGAGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2431.2147570485902819No Hit
ACGCCTGAGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2391.1947610477904418No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG2201.0997800439912018No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2201.0997800439912018No Hit
ACGCCTGAGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2061.0297940411917617No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT2041.0197960407918416No Hit
ACGCCTGAGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1910.9548090381923615No Hit
ACGCCTGAGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1870.9348130373925215No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1640.8198360327934412No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1610.8048390321935612No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1610.8048390321935612No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1530.7648470305938813No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1460.7298540291941612No Hit
ACGCCTGAGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1450.7248550289942012No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1410.7048590281943611No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1400.6998600279944012No Hit
ACGCCTGAGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1400.6998600279944012No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1370.6848630273945211No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1350.674865026994601No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT1280.639872025594881No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1260.629874025194961No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC1220.609878024395121No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1200.599880023995201No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1180.589882023595281No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1110.5548890221955609No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1100.5498900219956009No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA1020.5098980203959208No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1020.5098980203959208No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1010.5048990201959609No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1000.49990001999600076No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC980.4899020195960808No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC950.47490501899620075No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC940.4699060187962407No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA910.4549090181963607No Hit
ACGCCTGAGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG890.4449110177964407No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA890.4449110177964407No Hit
ACGCCTGAGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA890.4449110177964407No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC870.4349130173965207No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT790.39492101579684064No Hit
ACGCCTGAGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG770.38492301539692064No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC770.38492301539692064No Hit
ACGCCTGAGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA760.3799240151969606No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC750.3749250149970006No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT740.3699260147970406No Hit
ACGCCTGAGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA730.3649270145970806No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC680.33993201359728054No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT660.32993401319736054No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA630.3149370125974805No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.3049390121975605No Hit
ACGCCTGAGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA600.2999400119976005No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT590.2949410117976405No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT590.2949410117976405No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT580.28994201159768046No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT570.28494301139772044No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC570.28494301139772044No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC540.2699460107978404No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT540.2699460107978404No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT530.26494701059788045No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT530.26494701059788045No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA520.2599480103979204No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC520.2599480103979204No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT510.2549490101979604No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA500.24995000999800038No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT500.24995000999800038No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC500.24995000999800038No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT490.2449510097980404No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA480.23995200959808036No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT480.23995200959808036No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT470.23495300939812036No Hit
ATTCCTTCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT460.2299540091981604No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA450.22495500899820034No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT440.21995600879824037No Hit
ATTCCTTCACCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT440.21995600879824037No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC440.21995600879824037No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA420.20995800839832035No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC420.20995800839832035No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC410.2049590081983603No Hit
ACGCCTGAGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA400.19996000799840033No Hit
ATTCCTTCACCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT400.19996000799840033No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA400.19996000799840033No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC380.1899620075984803No Hit
ACGCCTGAGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA380.1899620075984803No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC370.1849630073985203No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT370.1849630073985203No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCT370.1849630073985203No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT370.1849630073985203No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA360.1799640071985603No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT360.1799640071985603No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA360.1799640071985603No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT360.1799640071985603No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT360.1799640071985603No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA350.1749650069986003No Hit
ACGCCTGAGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA350.1749650069986003No Hit
ATTCCTTCACCGGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACC340.16996600679864027No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT330.16496700659868027No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC330.16496700659868027No Hit
ATTCCTTCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT330.16496700659868027No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT330.16496700659868027No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT330.16496700659868027No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT330.16496700659868027No Hit
ATTCCTTCACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT330.16496700659868027No Hit
ATTCCTTCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCTAGCT320.15996800639872025No Hit
ACGCCTGAGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA320.15996800639872025No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC310.15496900619876025No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT310.15496900619876025No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT300.14997000599880025No Hit
ACGCCTGAGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA290.14497100579884023No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG290.14497100579884023No Hit
ATTCCTTCACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT280.13997200559888023No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCC280.13997200559888023No Hit
ATTCCTTCACCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC280.13997200559888023No Hit
ACGCCTGAGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA280.13997200559888023No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACATATGGGGCGAGCGTTA280.13997200559888023No Hit
ATTCCTTCACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT270.1349730053989202No Hit
ATTCCTTCACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC270.1349730053989202No Hit
ATTCCTTCACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT270.1349730053989202No Hit
ACGCCTGAGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA270.1349730053989202No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC260.1299740051989602No Hit
ACGCCTGAGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG240.11997600479904018No Hit
ACGCCTGAGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA240.11997600479904018No Hit
ATTCCTTCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT240.11997600479904018No Hit
ACGCCTGAGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG240.11997600479904018No Hit
ACGCCTGAGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA230.1149770045990802No Hit
ATTCCTTCACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT230.1149770045990802No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT230.1149770045990802No Hit
ATTCCTTCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCC230.1149770045990802No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC230.1149770045990802No Hit
ACGCCTGAGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA220.10997800439912019No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT220.10997800439912019No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC220.10997800439912019No Hit
ATTCCTTCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT220.10997800439912019No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA220.10997800439912019No Hit
ATTCCTTCACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT210.10497900419916018No Hit
ATTCCTTCACCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC210.10497900419916018No Hit
ATTCCTTCACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT210.10497900419916018No Hit
ACGCCTGAGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA210.10497900419916018No Hit
ATTCCTTCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT210.10497900419916018No Hit
ATTCCTTCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCC210.10497900419916018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)