FastQCFastQC Report
Sun 21 May 2023
EGAF00007837571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45880
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCGCCTCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT23465.11333914559721No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG12842.798605056669573No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG11722.5544899738448126No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG11012.399738448125545No Hit
ATTCGCCTCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT10872.36922406277245No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9672.1076721883173497No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA9482.066259808195292No Hit
ATTCGCCTCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT9342.0357454228421967No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8651.8853530950305144No Hit
ATTCGCCTCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT8421.8352223190932868No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA8221.7916303400174367No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7881.7175239755884915No Hit
ATTCGCCTCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6811.4843068875326941No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6781.4777680906713164No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG6561.4298169136878816No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6501.4167393199651264No Hit
ATTCGCCTCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6161.3426329555361813No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG5991.305579773321709No Hit
ATTCGCCTCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5951.2968613775065387No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4911.0701830863121187No Hit
ATTCGCCTCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4671.0178727114210984No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4510.9829991281604185No Hit
ATTCGCCTCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT4490.9786399302528335No Hit
ATTCGCCTCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4190.9132519616390585No Hit
ATTCGCCTCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4090.8914559721011335No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3800.8282476024411508No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3690.8042720139494333No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3580.7802964254577158No Hit
ATTCGCCTCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3450.7519616390584133No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3450.7519616390584133No Hit
ATTCGCCTCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3240.7061900610287707No Hit
ATTCGCCTCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT3170.6909328683522232No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3170.6909328683522232No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3120.6800348735832606No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3090.6734960767218832No Hit
ATTCGCCTCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3020.6582388840453357No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2910.6342632955536182No Hit
ATTCGCCTCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2800.6102877070619006No Hit
ATTCGCCTCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2680.5841325196163906No Hit
ATTCGCCTCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2550.555797733217088No Hit
ATTCGCCTCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2480.5405405405405406No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2470.5383609415867481No Hit
ATTCGCCTCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2460.5361813426329555No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2430.5296425457715781No Hit
ATTCGCCTCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2390.520924149956408No Hit
ATTCGCCTCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2290.499128160418483No Hit
ATTCGCCTCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2250.49040976460331304No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2250.49040976460331304No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2220.4838709677419355No Hit
ATTCGCCTCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2220.4838709677419355No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2210.48169136878814295No Hit
ATTCGCCTCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2070.451176983435048No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1980.4315605928509154No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1910.41630340017436795No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1860.40540540540540543No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1850.4032258064516129No Hit
ATTCGCCTCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1850.4032258064516129No Hit
ATTCGCCTCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1850.4032258064516129No Hit
ATTCGCCTCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1830.3988666085440279No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1800.3923278116826504No Hit
ATTCGCCTCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1770.38578901482127287No Hit
ATTCGCCTCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1770.38578901482127287No Hit
ATTCGCCTCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1720.37489102005231034No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1670.36399302528334787No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1560.3400174367916303No Hit
ATTCGCCTCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1520.33129904097646035No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1520.33129904097646035No Hit
ATTCGCCTCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1520.33129904097646035No Hit
ATTCGCCTCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1510.32911944202266785No Hit
ATTCGCCTCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1490.3247602441150828No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1470.3204010462074978No Hit
ATTCGCCTCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1450.3160418482999128No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1400.3051438535309503No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1390.3029642545771578No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1390.3029642545771578No Hit
ATTCGCCTCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.2986050566695728No Hit
ATTCGCCTCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1340.2920662598081953No Hit
ATTCGCCTCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1320.2877070619006103No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1250.2724498692240628No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1250.2724498692240628No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1240.2702702702702703No Hit
ATTCGCCTCCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT1220.26591107236268524No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1170.25501307759372277No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1140.24847428073234523No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1130.24629468177855274No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1120.24411508282476024No Hit
ATTCGCCTCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1110.24193548387096775No Hit
ATTCGCCTCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1040.2266782911944202No Hit
ATTCGCCTCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1040.2266782911944202No Hit
ATTCGCCTCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1020.22231909328683525No Hit
ATTCGCCTCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1010.22013949433304275No Hit
ATTCGCCTCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1010.22013949433304275No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA940.2048823016564952No Hit
ATTCGCCTCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT930.20270270270270271No Hit
ATTCGCCTCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT930.20270270270270271No Hit
ATTCGCCTCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.20052310374891022No Hit
ATTCGCCTCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT910.1983435047951177No Hit
ATTCGCCTCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT910.1983435047951177No Hit
ATTCGCCTCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT870.1896251089799477No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA840.18308631211857018No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA840.18308631211857018No Hit
ATTCGCCTCCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT840.18308631211857018No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA830.1809067131647777No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA820.1787271142109852No Hit
ATTCGCCTCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT810.17654751525719267No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA800.17436791630340018No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA770.16782911944202267No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA740.16129032258064516No Hit
ATTCGCCTCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT740.16129032258064516No Hit
ATTCGCCTCCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT730.15911072362685266No Hit
ATTCGCCTCCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT730.15911072362685266No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA720.15693112467306014No Hit
ATTCGCCTCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT710.15475152571926767No Hit
ATTCGCCTCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT710.15475152571926767No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA690.15039232781168266No Hit
ATTCGCCTCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC690.15039232781168266No Hit
ATTCGCCTCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC680.14821272885789014No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA660.14385353095030515No Hit
ATTCGCCTCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.13949433304272016No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA630.13731473408892764No Hit
ATTCGCCTCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC630.13731473408892764No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA630.13731473408892764No Hit
ATTCGCCTCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT620.13513513513513514No Hit
ATTCGCCTCCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT610.13295553618134262No Hit
ATTCGCCTCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT610.13295553618134262No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA600.13077593722755013No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA600.13077593722755013No Hit
ATTCGCCTCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC590.12859633827375763No Hit
ATTCGCCTCCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT590.12859633827375763No Hit
ATTCGCCTCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT580.12641673931996514No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA580.12641673931996514No Hit
ATTCGCCTCCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT570.12423714036617262No Hit
ATTCGCCTCCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC560.12205754141238012No Hit
ATTCGCCTCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT550.11987794245858763No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA540.11769834350479512No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA540.11769834350479512No Hit
ATTCGCCTCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.11551874455100263No Hit
ATTCGCCTCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.1133391455972101No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG510.11115954664341762No Hit
ATTCGCCTCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC510.11115954664341762No Hit
ATTCGCCTCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.11115954664341762No Hit
ATTCGCCTCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT490.1068003487358326No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA480.1046207497820401No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA470.1024411508282476No Hit
ATTCGCCTCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.1024411508282476No Hit
ATTCGCCTCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC460.10026155187445511No Hit
ATTCGCCTCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC460.10026155187445511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)