FastQCFastQC Report
Sun 21 May 2023
EGAF00007837740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48788
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA17323.5500532917930636No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA15763.2303025334098545No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT14162.9023530376322046No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11152.2853980487005003No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG10762.205460359104698No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10412.133721406903337No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10012.051734032958924No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9731.9943428711978355No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8051.6499959006313027No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7831.6049028449618759No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7711.5803066327785522No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT7511.539312945806346No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7491.5352135771091253No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6981.4306796753299993No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6711.3753381979175205No Hit
ATACCGGTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6211.272853980487005No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5871.2031647126342544No Hit
ATACCGGTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5761.180618184799541No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5671.162171025662048No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT5511.129376076084283No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5181.0617364925801427No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4991.0227924899565466No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4820.9879478560301713No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4690.9613019594982373No Hit
ATACCGGTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4670.9572025908010167No Hit
ATACCGGTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4170.8547183733705009No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4150.8506190046732803No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4080.8362712142330081No Hit
ATACCGGTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4080.8362712142330081No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC4000.8198737394441257No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3870.7932278429121916No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3830.7850291055177502No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3620.7419857341969337No Hit
ATACCGGTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3400.6968926785275067No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3380.6927933098302861No Hit
ATACCGGTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3310.6784455193900139No Hit
ATACCGGTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3230.6620480446011314No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT3030.6210543576289251No Hit
ATACCGGTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2960.606706567188653No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2860.5862097237025499No Hit
ATACCGGTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2800.5739116176108879No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2780.5698122489136673No Hit
ATACCGGTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2760.5657128802164467No Hit
ATACCGGTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2620.5370172993359023No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2620.5370172993359023No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2600.5329179306386816No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2430.49807329671230627No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2350.48167582192342373No Hit
ATACCGGTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2320.4755267688775928No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2280.4673280314831516No Hit
ATACCGGTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2260.463228662785931No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2230.4570796097401No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC2200.45093055669426907No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2140.43863245060260725No Hit
ATACCGGTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1980.4058375010248421No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1940.3976387636304009No Hit
ATACCGGTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1900.38944002623595964No Hit
ATACCGGTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1820.37304255144707715No Hit
ATACCGGTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1750.358694761006805No Hit
ATACCGGTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1720.352545707960974No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1720.352545707960974No Hit
ATACCGGTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1700.34844633926375335No Hit
ATACCGGTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1700.34844633926375335No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1670.34229728621792244No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1610.32999918012626056No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT1540.3156513896859884No Hit
ATACCGGTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1540.3156513896859884No Hit
ATACCGGTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1530.313601705337378No Hit
ATACCGGTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1510.3095023366401574No Hit
ATACCGGTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1480.3033532835943265No Hit
ATACCGGTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1470.30130359924571615No Hit
ATACCGGTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1420.29105517750266463No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1400.28695580880544397No Hit
ATACCGGTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1390.28490612445683366No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1370.28080675575961306No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1360.2787570714110027No Hit
ATACCGGTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1330.2726080183651718No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1310.2685086496679511No Hit
ATACCGGTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1220.2500614905304583No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA1210.24801180618184798No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC1200.24596212183323768No Hit
ATACCGGTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.24391243748462738No Hit
ATACCGGTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1130.2316143313929655No Hit
ATACCGGTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1120.2295646470443552No Hit
ATACCGGTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1120.2295646470443552No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA1120.2295646470443552No Hit
ATACCGGTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1110.22751496269574484No Hit
ATACCGGTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1110.22751496269574484No Hit
ATACCGGTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1100.22546527834713453No Hit
ATACCGGTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1060.2172665409526933No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCT1050.21521685660408296No Hit
ATACCGGTTCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC1010.20701811920964172No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1010.20701811920964172No Hit
ATACCGGTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT910.18652127572353858No Hit
ATACCGGTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC910.18652127572353858No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA910.18652127572353858No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA900.18447159137492827No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT900.18447159137492827No Hit
ATACCGGTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT890.18242190702631797No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT880.18037222267770764No Hit
ATACCGGTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT860.176272853980487No Hit
ATACCGGTTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT850.17422316963187667No Hit
ATACCGGTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT850.17422316963187667No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA840.17217348528326637No Hit
ATACCGGTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT830.17012380093465607No Hit
ATACCGGTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC830.17012380093465607No Hit
ATACCGGTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT820.16807411658604574No Hit
ATACCGGTTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC820.16807411658604574No Hit
ATACCGGTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT810.16602443223743543No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA780.1598753791916045No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA780.1598753791916045No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA760.15577601049438386No Hit
ATACCGGTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT760.15577601049438386No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC730.14962695744855292No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA720.14757727309994262No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA710.14552758875133232No Hit
ATACCGGTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT710.14552758875133232No Hit
ATACCGGTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT700.14347790440272198No Hit
ATACCGGTTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT690.14142822005411168No Hit
ATACCGGTTCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC660.13527916700828072No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA660.13527916700828072No Hit
ATACCGGTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT650.1332294826596704No Hit
ATACCGGTTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.13117979831106008No Hit
ATACCGGTTCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT640.13117979831106008No Hit
ATACCGGTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.12708042961383947No Hit
ATACCGGTTCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCACGCT620.12708042961383947No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA610.12503074526522914No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA610.12503074526522914No Hit
ATACCGGTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.12503074526522914No Hit
ATACCGGTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC600.12298106091661884No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA580.1188816922193982No Hit
ATACCGGTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC580.1188816922193982No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCT580.1188816922193982No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA570.1168320078707879No Hit
ATACCGGTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.1147823235221776No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA560.1147823235221776No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT560.1147823235221776No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA560.1147823235221776No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGGGGCGAGCGTTG550.11273263917356727No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA550.11273263917356727No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA540.11068295482495696No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA530.10863327047634665No Hit
ATACCGGTTCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT530.10863327047634665No Hit
ATACCGGTTCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT530.10863327047634665No Hit
ATACCGGTTCCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC530.10863327047634665No Hit
ATACCGGTTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC530.10863327047634665No Hit
ATACCGGTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT520.10658358612773633No Hit
ATACCGGTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT520.10658358612773633No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGGGGCGAGCGTTG520.10658358612773633No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG510.10453390177912601No Hit
ATACCGGTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.10453390177912601No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA510.10453390177912601No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA510.10453390177912601No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA500.10248421743051571No Hit
ATACCGGTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.10248421743051571No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA490.10043453308190538No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG490.10043453308190538No Hit
ATACCGGTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT490.10043453308190538No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)