FastQCFastQC Report
Sun 21 May 2023
EGAF00007837780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50228
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG20013.983833718244804No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT18553.693159194075018No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16433.2710838576093013No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG15093.0043003902205943No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG13402.6678346738870746No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA10772.1442223461017758No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10732.136258660508083No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT10682.126304053515967No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG10502.09046746834435No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT9861.9630484988452657No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8951.7818746515887551No Hit
ATACCGGTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8881.7679382017997929No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8531.6982559528549812No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8001.5927371187385524No Hit
ATACCGGTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7371.467309070637891No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6861.3657720793183086No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6441.2821533805845344No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6031.2005256032491838No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5671.1288524329059488No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5631.1208887473122562No Hit
ATACCGGTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5591.1129250617185633No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA5431.0810703193437923No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT5231.0412518913753286No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5151.0253245201879428No Hit
ATACCGGTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT4780.9516604284462848No Hit
ATACCGGTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4720.9397149000557458No Hit
ATACCGGTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4500.8959146292904356No Hit
ATACCGGTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4230.8421597515330094No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4080.8122959305566616No Hit
ATACCGGTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3820.7605319741976587No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3650.7266863104244644No Hit
ATACCGGTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT3650.7266863104244644No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA3500.6968224894481165No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3400.6769132754638847No Hit
ATACCGGTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3380.6729314326670383No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3120.6211674763080354No Hit
ATACCGGTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3040.6052401051206499No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2890.575376284144302No Hit
ATACCGGTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2830.5634307557537629No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA2820.5614398343553396No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2780.5534761487616469No Hit
ATACCGGTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2650.5275941705821454No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2610.5196304849884527No Hit
ATACCGGTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT2470.491757585410528No Hit
ATACCGGTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2440.4857848212152584No Hit
ATACCGGTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2430.48379389981683524No Hit
ATACCGGTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2420.481802978418412No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT2330.4638846858326033No Hit
ATACCGGTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2290.4559210002389106No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2130.42406625786413954No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA2070.4121207294736004No Hit
ATACCGGTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2070.4121207294736004No Hit
ATACCGGTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2060.41012980807517724No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2010.40017520108306126No Hit
ATACCGGTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1940.3862387512940989No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1890.3762841443019829No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1880.37429322290355976No Hit
ATACCGGTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1820.3623476945130206No Hit
ATACCGGTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1740.3464203233256351No Hit
ATACCGGTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1740.3464203233256351No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1740.3464203233256351No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1730.34442940192721194No Hit
ATACCGGTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1660.3304929521382496No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1640.3265111093414032No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1630.32452018794298004No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1510.30062913116190176No Hit
ATACCGGTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1440.2866926813729394No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1410.2807199171776698No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCT1400.27872899577924665No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1370.2727562315839771No Hit
ATACCGGTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1350.26877438878713067No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1330.2647925459902843No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1320.26280162459186107No Hit
ATACCGGTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1280.25483793899816837No Hit
ATACCGGTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1260.250856096201322No Hit
ATACCGGTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1240.24687425340447558No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1210.240901489209206No Hit
ATACCGGTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT1200.23891056781078285No Hit
ATACCGGTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.23492872501393644No Hit
ATACCGGTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.23094688221709006No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1160.23094688221709006No Hit
ATACCGGTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1120.22298319662339727No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1120.22298319662339727No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1100.21900135382655092No Hit
ATACCGGTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1090.21701043242812776No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1080.21501951102970454No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1080.21501951102970454No Hit
ATACCGGTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1070.21302858963128135No Hit
ATACCGGTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1070.21302858963128135No Hit
ATACCGGTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.20705582543601178No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT1030.20506490403758862No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1010.2010830612407422No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA990.19710121844389583No Hit
ATACCGGTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCT970.19311937564704945No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA950.18913753285020307No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA950.18913753285020307No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA950.18913753285020307No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA910.1811738472565103No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC900.17918292585808712No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA900.17918292585808712No Hit
ATACCGGTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT890.17719200445966393No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA880.17520108306124074No Hit
ATACCGGTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT860.17121924026439436No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT820.1632555546707016No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA800.15927371187385522No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA800.15927371187385522No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT790.15728279047543203No Hit
ATACCGGTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT790.15728279047543203No Hit
ATACCGGTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC780.15529186907700884No Hit
ATACCGGTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT770.15330094767858565No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA760.15131002628016246No Hit
ATACCGGTTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.14732818348331608No Hit
ATACCGGTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT730.1453372620848929No Hit
ATACCGGTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC730.1453372620848929No Hit
ATACCGGTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.1433463406864697No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA720.1433463406864697No Hit
ATACCGGTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT700.13936449788962332No Hit
ATACCGGTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT700.13936449788962332No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG690.13737357649120013No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT690.13737357649120013No Hit
ATACCGGTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACT670.13339173369435375No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA670.13339173369435375No Hit
ATACCGGTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCT650.12940989089750737No Hit
ATACCGGTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCATGCT640.12741896949908418No Hit
ATACCGGTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT640.12741896949908418No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA630.125428048100661No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA620.12343712670223779No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA620.12343712670223779No Hit
ATACCGGTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCATGCT610.1214462053038146No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA590.11746436250696822No Hit
ATACCGGTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT590.11746436250696822No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG590.11746436250696822No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA570.11348251971012185No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA570.11348251971012185No Hit
ATACCGGTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.11348251971012185No Hit
ATACCGGTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT560.11149159831169864No Hit
ATACCGGTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT550.10950067691327546No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACT550.10950067691327546No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA550.10950067691327546No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA550.10950067691327546No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA540.10750975551485227No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA540.10750975551485227No Hit
ATACCGGTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC540.10750975551485227No Hit
ATACCGGTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT540.10750975551485227No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA520.10352791271800589No Hit
ATACCGGTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT520.10352791271800589No Hit
ATACCGGTTCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT520.10352791271800589No Hit
ATACCGGTTCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC520.10352791271800589No Hit
ATACCGGTTCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT510.1015369913195827No Hit
ATACCGGTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCT510.1015369913195827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)