FastQCFastQC Report
Sun 21 May 2023
EGAF00007837878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62274
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA24303.9021100298680027No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA22793.6596332337733246No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT18092.9049041333461796No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT15222.4440376401066257No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG14412.313967305777692No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG14322.2995150464078105No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA12992.0859427690528953No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA11831.8996692038410896No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG9801.573690464720429No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9421.5126698140475963No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT9311.4950059414844075No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8921.4323794842149211No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7791.2509233387930756No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7761.2461059190031152No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7311.1738446221537078No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA7241.1626039759771334No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA7221.1593923627838263No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT6841.0983717121109933No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6651.067861386774577No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6241.0020233163117833No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6231.00041750971513No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6000.9634839579920994No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT5840.9377910524456434No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG5640.9056749205125735No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5580.8960400809326524No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5280.8478658830330474No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4950.794874265343482No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT4860.7804220059736006No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4720.7579407136204516No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4700.7547291004271446No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4660.7483058740405305No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4550.730642001477342No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA4420.7097665157208466No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA4390.7049490959308861No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4240.6808619969810836No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3920.6294761858881716No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3800.6102065067283297No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3780.6069948935350227No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3680.5909368275684876No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3500.5620323088287247No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3470.5572148890387641No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3420.5491858560554966No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3060.4913768185759707No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3000.4817419789960497No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2990.48013617239939627No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2860.45926068664290076No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2790.4480200404663262No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT2740.4399910074830587No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2730.43838520088640526No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA2690.43196197449979123No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2680.4303561679031377No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2670.42875036130648425No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2600.41750971512990975No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2580.41429810193660277No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2580.41429810193660277No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2570.4126922953399493No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2540.40787487554998875No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2460.39502842277676076No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2280.3661239040369978No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2270.3645180974403443No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA2250.3613064842470373No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2180.35006583807046276No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2150.3452484182805023No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2100.33721938529723483No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2080.3340077721039278No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA1990.3195555127340463No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1980.3179497061373928No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1950.3131322863474323No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1930.3099206731541253No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1890.30349744676751134No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1890.30349744676751134No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1880.30189164017085784No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1850.2970742203808973No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1800.28904518739762985No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1800.28904518739762985No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1780.2858335742043228No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1750.28101615441436234No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1740.27941034781770885No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1740.27941034781770885No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1710.2745929280277483No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1680.26977550823778784No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1640.2633522818511739No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1620.26014066865786684No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1620.26014066865786684No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1610.25853486206121334No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1600.25692905546455985No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1580.2537174422712528No Hit
ATCTCGCGGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1530.24568840928798535No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1500.24087098949802485No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1490.23926518290137136No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1410.22641873012814337No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1410.22641873012814337No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1390.22320711693483639No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1390.22320711693483639No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1370.21999550374152935No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1360.21838969714487586No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1330.21357227735491535No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1330.21357227735491535No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT1270.20393743777499437No Hit
ATCTCGCGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1230.19751421138838038No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.19751421138838038No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1200.19269679159841988No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1190.19109098500176638No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1180.1894851784051129No Hit
ATCTCGCGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1170.1878793718084594No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1170.1878793718084594No Hit
ATCTCGCGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.18627356521180588No Hit
ATCTCGCGGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1150.1846677586151524No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1150.1846677586151524No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT1140.1830619520184989No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC1110.1782445322285384No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1100.17663872563188487No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG1080.1734271124385779No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG1080.1734271124385779No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1070.1718213058419244No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1070.1718213058419244No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA1050.16860969264861742No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1030.1653980794553104No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1020.1637922728586569No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCT1010.16218646626200342No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1010.16218646626200342No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1010.16218646626200342No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT1000.1605806596653499No Hit
ATCTCGCGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1000.1605806596653499No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1000.1605806596653499No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA970.1557632398753894No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT950.15255162668208241No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT930.1493400134887754No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC930.1493400134887754No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA920.1477342068921219No Hit
ATCTCGCGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT920.1477342068921219No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA910.14612840029546842No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA910.14612840029546842No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT880.14131098050550792No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT870.13970517390885442No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA860.1380993673122009No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTATTTGCTCCCCACGCT860.1380993673122009No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT850.1364935607155474No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA840.13488775411889392No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC830.13328194752224043No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA820.13167614092558694No Hit
ATCTCGCGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT820.13167614092558694No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT810.13007033432893342No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC800.12846452773227993No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT770.12364710794231942No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA750.12043549474901243No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGAGGGCCCCAAGCGTTA740.11882968815235893No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA730.11722388155570543No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGAGGGCCCCAAGCGTTA730.11722388155570543No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTA730.11722388155570543No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC690.11080065516909145No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA680.10919484857243793No Hit
ATCTCGCGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT680.10919484857243793No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.10758904197578442No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT660.10598323537913093No Hit
ATCTCGCGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT660.10598323537913093No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC660.10598323537913093No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT660.10598323537913093No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTA650.10437742878247744No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT640.10277162218582393No Hit
ATCTCGCGGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT640.10277162218582393No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT640.10277162218582393No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.10277162218582393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)