FastQCFastQC Report
Sun 21 May 2023
EGAF00007837880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64945
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA23943.6861960120101624No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA23343.593810147047502No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA19192.9548079143890984No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA18612.8655015782585265No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG12331.8985295249826777No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG11791.8153822465162828No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT11261.7337747324659327No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG10041.5459234737085226No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9551.4704750173223498No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9231.4212025560089305No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT8831.3596119793671568No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7961.225652475171299No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7291.1224882592963277No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT7191.1070906151358841No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6821.0501193317422435No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6681.0285626299176227No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6521.003926399260913No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6511.0023866348448687No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6070.9346370005389175No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6020.9269381784586959No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6000.9238586496266071No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5780.8899838324736317No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5740.8838247748094542No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5660.8715066594810994No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5610.8638078374008777No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5370.8268534914158134No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5200.8006774963430595No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT5130.789899145430749No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5080.7822003233505274No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4860.7483255061975518No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4800.7390869197012857No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4630.7129109246285318No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4410.6790361074755563No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA4270.6574794056509353No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA4270.6574794056509353No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4260.655939641234891No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4230.651320347986758No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4200.6467010547386249No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT4130.6359227038263146No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3760.5789514204326738No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3730.5743321271845407No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3670.5650935406882748No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3500.5389175456155209No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3430.5281391947032105No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3380.5204403726229887No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3170.48810531988605743No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3170.48810531988605743No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3160.4865655554700131No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3150.4850257910539687No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3120.48040649780583566No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3100.47732696897374705No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT3080.4742474401416583No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3020.46500885364539224No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2940.4526907383170375No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2830.4357533297405497No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2800.43113403649241666No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2800.43113403649241666No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2730.4203556855801063No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2730.4203556855801063No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2720.41881592116406197No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2650.40803757025175147No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2620.4034182770036185No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2520.388020632843175No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2390.3680036954345985No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2390.3680036954345985No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2290.35260605127415506No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2250.34644699360997766No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2240.34490722919393335No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2200.33874817152975595No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2160.33258911386557855No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2130.3279698206174455No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2130.3279698206174455No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2120.32643005620140114No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2050.31565170528909076No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1960.30179382554469164No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1960.30179382554469164No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1930.2971745322965586No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1930.2971745322965586No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1910.29409500346446993No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1880.2894757102163369No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1810.2786973593040265No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1800.27715759488798214No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1790.2756178304719378No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1780.2740780660558934No Hit
TACCTCGTACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1780.2740780660558934No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1740.26791900839171606No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1720.2648394795596274No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1690.26022018631149435No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1650.254061128647317No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1630.2509815998152283No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1570.2417430133189622No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1480.2278851335745631No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1450.22326584032643007No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1410.21710678266225267No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1380.21248748941411966No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1370.2109477249980753No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1350.20786819616598662No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1320.20324890291785355No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1300.20016937408576488No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1300.20016937408576488No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1290.1986296096697205No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1280.1970898452536762No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1280.1970898452536762No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1260.1940103164215875No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1260.1940103164215875No Hit
TACCTCGTACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1220.1878512587574101No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.1878512587574101No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1200.18477172992532143No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1200.18477172992532143No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1160.17861267226114405No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1160.17861267226114405No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1110.1709138501809223No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1100.16937408576487797No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1100.16937408576487797No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1100.16937408576487797No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1050.16167526368465623No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1030.15859573485256756No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1030.15859573485256756No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1020.15705597043652322No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1000.15397644160443452No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA990.15243667718839018No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA980.15089691277234582No Hit
TACCTCGTACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT980.15089691277234582No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT960.14781738394025715No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA950.14627761952421278No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA930.1431980906921241No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG920.14165832627607977No Hit
TACCTCGTACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT920.14165832627607977No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG910.1401185618600354No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT890.1370390330279467No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA890.1370390330279467No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA880.13549926861190237No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA870.13395950419585803No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC870.13395950419585803No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT870.13395950419585803No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA860.1324197397798137No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT850.13087997536376936No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT850.13087997536376936No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA840.129340210947725No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC840.129340210947725No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT830.12780044653168066No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC810.12472091769959197No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT810.12472091769959197No Hit
TACCTCGTACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT800.12318115328354762No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC790.12164138886750328No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA790.12164138886750328No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA780.12010162445145892No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.12010162445145892No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT770.11856186003541458No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC760.11702209561937024No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA750.11548233120332589No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC750.11548233120332589No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA750.11548233120332589No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC730.11240280237123719No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT730.11240280237123719No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA730.11240280237123719No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA730.11240280237123719No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA720.11086303795519285No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG720.11086303795519285No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG720.11086303795519285No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC700.10778350912310417No Hit
TACCTCGTACCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT700.10778350912310417No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC700.10778350912310417No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT680.10470398029101549No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA680.10470398029101549No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG660.10162445145892678No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCT660.10162445145892678No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA650.10008468704288244No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)