FastQCFastQC Report
Sun 21 May 2023
EGAF00007837886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42737
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13713.2079930739172147No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT13413.137796288929967No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT10792.524744366708005No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10692.501345438378922No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9482.2182184055970238No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9082.1246226922806937No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA8201.9187121229847675No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7451.7432201605166484No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6981.6332451973699604No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6961.628565411704144No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6221.4554133420689332No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5941.3898963427475022No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5681.3290591290918876No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5531.293960736598264No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5381.2588623441046398No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5291.2378033086084657No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA5201.2167442731122915No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5081.1886655591173922No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT4981.1652666307883097No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4981.1652666307883097No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4811.1254884526288695No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4801.1231485597959614No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA4741.1091092027985119No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4301.0061539181505488No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4260.9967943468189158No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4010.9382970259962095No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3990.9336172403303928No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3680.861080562510237No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3460.8096029201862553No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3430.8025832416875307No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3320.7768444205255399No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3320.7768444205255399No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3310.7745045276926317No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3250.7604651706951822No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3170.7417460280319161No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3120.7300465638673749No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3060.7160072068699254No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2810.657509886047219No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA2810.657509886047219No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2590.6060322437232374No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2530.591992886725788No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG2520.5896529938928797No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2510.5873131010599715No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2480.5802934225612467No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2470.5779535297283385No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2460.5756136368954302No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2400.5615742798979807No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA2360.5522147085663477No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2250.5264758874043569No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2250.5264758874043569No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2250.5264758874043569No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2100.4913774949107331No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2090.4890376020778248No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT2080.4866977092449166No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2020.47265835224746705No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2010.4703184594145588No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1990.4656386737487423No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1750.4094812457589443No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1710.4001216744273112No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1690.3954418887614947No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1660.38842221026276996No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1650.3860823174298617No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1620.37906263893113695No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1580.36970306759950394No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1530.3580036034349627No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1530.3580036034349627No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1500.3509839249362379No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1490.34864403210332967No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1470.34396424643751317No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1460.3416243536046049No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1380.3229052109413389No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1360.3182254252755224No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1340.3135456396097059No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1290.3018461754451646No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1290.3018461754451646No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1270.29716638977934806No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1220.2854669256148068No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1210.2831270327818986No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1200.28078713994899035No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1150.2690876757844491No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1120.2620679972857243No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1110.2597281044528161No Hit
ATCTCGCGGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1100.2573882116199078No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1090.2550483187869996No Hit
ATCTCGCGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1080.25270842595409126No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1080.25270842595409126No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1050.24568874745536656No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1050.24568874745536656No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1040.2433488546224583No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1040.2433488546224583No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1010.23632917612373353No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1000.2339892832908253No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT980.22930949762500877No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.2176100334604675No Hit
ATCTCGCGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT920.21527014062755925No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC920.21527014062755925No Hit
ATCTCGCGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT910.212930247794651No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG890.20825046212883452No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT890.20825046212883452No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT890.20825046212883452No Hit
ATCTCGCGGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT860.20123078363010974No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA850.1988908907972015No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA830.19421110513138498No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA820.19187121229847673No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA800.18719142663266022No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA800.18719142663266022No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA790.18485153379975197No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT780.18251164096684372No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA750.17549196246811896No Hit
ATCTCGCGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT750.17549196246811896No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT750.17549196246811896No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA750.17549196246811896No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.17081217680230246No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC720.1684722839693942No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC680.1591127126377612No Hit
ATCTCGCGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT660.15443292697194466No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA650.15209303413903644No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT650.15209303413903644No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA630.14741324847321993No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC630.14741324847321993No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT630.14741324847321993No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT630.14741324847321993No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT630.14741324847321993No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA610.1427334628074034No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA610.1427334628074034No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCCAGCT580.13571378430867867No Hit
ATCTCGCGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT580.13571378430867867No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA580.13571378430867867No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT580.13571378430867867No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC550.1286941058099539No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC550.1286941058099539No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT530.1240143201441374No Hit
ATCTCGCGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT520.12167442731122916No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT510.11933453447832089No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA500.11699464164541265No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.11699464164541265No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT490.11465474881250438No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC490.11465474881250438No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA480.11231485597959613No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA470.10997496314668789No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT470.10997496314668789No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.10997496314668789No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.10997496314668789No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT470.10997496314668789No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC450.10529517748087139No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC450.10529517748087139No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC450.10529517748087139No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT450.10529517748087139No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCCAGCT450.10529517748087139No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC440.10295528464796312No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA440.10295528464796312No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA440.10295528464796312No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC440.10295528464796312No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT430.10061539181505487No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT430.10061539181505487No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT430.10061539181505487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)