FastQCFastQC Report
Sun 21 May 2023
EGAF00007837904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50170
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA27345.4494717958939605No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG19763.938608730316923No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT16393.2668925652780545No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA15313.0516244767789513No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA13152.6210882997807454No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG12522.4955152481562686No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10672.1267689854494716No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8551.704205700617899No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8481.6902531393262907No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT8471.6882599162846321No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8101.6145106637432727No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7321.4590392664939207No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6451.2856288618696432No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6421.2796491927446683No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6121.2198525014949173No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT5741.1441100259118997No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT5611.1181981263703409No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5421.080326888578832No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5281.052421765995615No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5271.0504285429539566No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4990.9946182977875225No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC4650.9268487143711381No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4540.9049232609128962No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4470.8909706996212876No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4070.8112417779549531No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3930.7833366553717361No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3810.7594179788718357No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3700.7374925254135938No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3640.7255331871636436No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3550.7075941797887184No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3480.6936416184971098No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3320.6617500498305761No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3120.6218855889974088No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3030.6039465816224836No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2990.5959736894558502No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2920.5820211281642416No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2910.5800279051225832No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2890.5760414590392665No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2840.5660753438309747No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2830.5640821207893163No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2790.5561092286226829No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC2770.5521227825393662No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2740.546143113414391No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2690.5361769982060993No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2560.5102650986645405No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2480.4943193143312737No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2410.48036675303966514No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2300.45844129958142316No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC2280.45445485349810644No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2260.4504684074147897No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2110.4205700617899143No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT2060.41060394658162247No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2030.40462427745664736No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC2000.39864460833167237No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1870.3727327087901136No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1860.3707394857484553No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1830.36475981662348017No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1820.3627665935818218No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1770.35280047837353No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1770.35280047837353No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1770.35280047837353No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1750.3488140322902133No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1730.3448275862068966No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1730.3448275862068966No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1660.33087502491528803No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1660.33087502491528803No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1640.3268885788319713No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1640.3268885788319713No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1580.31492924058202115No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1550.30894957145704605No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1530.3049631253737293No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1520.302969902332071No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1510.3009766792904126No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1470.29300378712377917No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1450.2890173410404624No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1430.28503089495714573No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1380.2750647797488539No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1370.2730715567071955No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1360.27107833366553713No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1330.2650986645405621No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1330.2650986645405621No Hit
ATCTCGCGGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1310.26111221845724536No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1300.25911899541558703No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1290.25712577237392864No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1250.2491528802072952No Hit
ATCTCGCGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1220.24317321108232012No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC1210.24117998804066174No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1170.23320709587402833No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.23121387283236997No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1130.22523420370739486No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA1120.2232409806657365No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1100.21925453458241975No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1070.2132748654574447No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1070.2132748654574447No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1050.20928841937412795No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1050.20928841937412795No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1030.20530197329081123No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1030.20530197329081123No Hit
ATCTCGCGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1010.20131552720749452No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1000.19932230416583618No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1000.19932230416583618No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT970.19334263504086108No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT950.18935618895754436No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA940.18736296591588597No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT900.17939007374925253No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC860.1714171815826191No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC830.16543751245764401No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA820.16344428941598566No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT820.16344428941598566No Hit
ATCTCGCGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT810.16145106637432727No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA800.15945784333266894No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT800.15945784333266894No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC800.15945784333266894No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC790.15746462029101058No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA790.15746462029101058No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA780.1554713972493522No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC770.15347817420769383No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC770.15347817420769383No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA760.1514849511660355No Hit
ATCTCGCGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT760.1514849511660355No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA750.1494917281243771No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT740.14749850508271875No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA730.1455052820410604No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC720.14351205899940203No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC710.14151883595774367No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC700.1395256129160853No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT690.13753238987442695No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA690.13753238987442695No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT690.13753238987442695No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT680.13553916683276857No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG680.13553916683276857No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.13354594379111023No Hit
ATCTCGCGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT670.13354594379111023No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA660.13155272074945187No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA660.13155272074945187No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA650.12955949770779351No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA650.12955949770779351No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTATTTGCTCCCCACGCT630.1255730516244768No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA630.1255730516244768No Hit
ATCTCGCGGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT620.12357982858281842No Hit
ATCTCGCGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT620.12357982858281842No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT620.12357982858281842No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT620.12357982858281842No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT620.12357982858281842No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA610.12158660554116006No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA590.11760015945784333No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA590.11760015945784333No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA580.11560693641618498No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG580.11560693641618498No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT580.11560693641618498No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA560.11162049033286825No Hit
ATCTCGCGGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT550.10962726729120988No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA540.10763404424955153No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA530.10564082120789317No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC520.1036475981662348No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA520.1036475981662348No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCCGTTTGCTCCCCTGGCT510.10165437512457644No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA510.10165437512457644No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.10165437512457644No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC510.10165437512457644No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA510.10165437512457644No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA510.10165437512457644No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)