FastQCFastQC Report
Sun 21 May 2023
EGAF00007837906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39037
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA15473.962906985680252No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT14703.7656582216871173No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13673.501805978943054No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT12233.1329251735532955No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8942.2901350001280836No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8512.1799830929630866No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7781.992981018008556No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6691.713758741706586No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6541.6753336578118196No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6291.6112918513205419No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6081.557496733867869No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5761.4755232215590337No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5081.301329507902759No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT5061.2962061633834567No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4581.173245894920204No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4511.1553141891026462No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA4461.1425058278043907No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4371.1194507774675309No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4271.09383405487102No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4271.09383405487102No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4101.0502856264569511No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3931.0067371980428823No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3870.9913671644849757No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3660.9375720470323028No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3540.9068319799164895No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3520.9017086353971873No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3460.8863386018392807No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3210.8222967953480033No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3170.8120501063093987No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3130.8018034172707944No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3120.7992417450111432No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA3070.7864333837128877No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA3030.7761866946742834No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2960.7582549888567256No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2800.7172682327023081No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2720.6967748546250992No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2630.6737198042882394No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2530.6481030816917284No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2530.6481030816917284No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2530.6481030816917284No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2520.6455414094320773No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2520.6455414094320773No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2510.6429797371724262No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2500.6404180649127751No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2430.6224863590952173No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2410.6173630145759151No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2350.6019929810180086No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2320.5943079642390553No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2200.5635678971232421No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2170.5558828803442888No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2050.5251428132284756No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2030.5200194687091734No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2020.5174577964495222No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1960.5020877628916156No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1940.4969644183723134No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA1800.46110100673719806No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1790.45853933447754697No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1770.4534159899582447No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1760.45085431769859363No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1740.4457309731792914No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1710.4380459564003381No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1600.40986756154417603No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1550.39705920024592056No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1430.36631913313010733No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1360.3483874273125496No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1350.34582575505289853No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1330.34070241053359634No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1330.34070241053359634No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1310.3355790660142941No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1300.333017393754643No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1290.33045572149499197No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1250.32020903245638754No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1220.3125240156774342No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1190.30483899889848093No Hit
ATCTCGCGGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1190.30483899889848093No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1180.30227732663882984No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.29715398211952765No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1110.2843456208212721No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1100.28178394856162103No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1090.2792222763019699No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1060.2715372595230166No Hit
ATCTCGCGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1050.2689755872633655No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT900.23055050336859903No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA890.2279888311089479No Hit
ATCTCGCGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT870.2228654865896457No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCC860.22030381432999463No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT850.21774214207034354No Hit
ATCTCGCGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT820.21005712529139023No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA810.2074954530317391No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA810.2074954530317391No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA800.20493378077208801No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA800.20493378077208801No Hit
ATCTCGCGGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT770.19724876399313473No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT720.1844404026948792No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT690.17675538591592593No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT680.1741937136562748No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA680.1741937136562748No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT670.17163204139662372No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT660.16907036913697263No Hit
ATCTCGCGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT650.1665086968773215No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA640.1639470246176704No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC620.15882368009836823No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.153700335579066No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA600.153700335579066No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC590.15113866331941492No Hit
ATCTCGCGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT590.15113866331941492No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA590.15113866331941492No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT560.1434536465404616No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA550.14089197428081052No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA550.14089197428081052No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA540.13833030202115942No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA540.13833030202115942No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC540.13833030202115942No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.13833030202115942No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.1332069575018572No Hit
ATCTCGCGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT520.1332069575018572No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.1332069575018572No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA500.128083612982555No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTG500.128083612982555No Hit
ATCTCGCGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT490.1255219407229039No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT490.1255219407229039No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA480.12296026846325282No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.12039859620360172No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA470.12039859620360172No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTG460.11783692394395061No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA460.11783692394395061No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA450.11527525168429952No Hit
ATCTCGCGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT450.11527525168429952No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT450.11527525168429952No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT450.11527525168429952No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT430.11015190716499731No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA420.10759023490534621No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA420.10759023490534621No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC410.10502856264569511No Hit
ATCTCGCGGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT410.10502856264569511No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT410.10502856264569511No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG400.10246689038604401No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA400.10246689038604401No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA400.10246689038604401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)