FastQCFastQC Report
Sun 21 May 2023
EGAF00007837908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46256
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA18283.951919750951228No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT15333.3141646489104115No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14713.1801279833967486No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT10572.2851089588377724No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9582.071082670356278No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9081.9629885852646145No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8361.8073331027326185No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT8261.7857142857142856No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7471.6149256312694569No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7421.6041162227602905No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7381.5954686959529574No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6451.3944136976824628No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6071.3122621930127982No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT6061.310100311310965No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5271.1393116568661363No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5161.1155309581459703No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5151.1133690764441369No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5111.104721549636804No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4651.0052749913524732No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4610.9966274645451402No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4520.9771705292286406No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4450.9620373573158076No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4180.9036665513663092No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4020.8690764441369769No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3950.8539432722241438No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3890.8409719820131443No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3760.8128675198893117No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3630.7847630577654792No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3520.7609823590453131No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3470.7501729505361466No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3440.7436873054306469No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3140.6788308543756486No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3100.6701833275683156No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3050.6593739190591491No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3050.6593739190591491No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3010.650726392251816No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2930.6334313386371497No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2860.6182981667243168No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA2840.6139744033206502No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2790.6031649948114839No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2750.5945174680041508No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2610.5642511241784849No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2560.5534417156693185No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2550.5512798339674853No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2540.549117952265652No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2360.5102040816326531No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2280.49290902801798686No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2240.48426150121065376No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA2210.47777585610515394No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2180.4712902109996541No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2160.46696644759598754No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2140.462642684192321No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2130.4604808024904877No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2070.44750951227948804No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1960.423728813559322No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1950.4215669318574888No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1930.4172431684538222No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1910.4129194050501556No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1910.4129194050501556No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1850.39994811483915604No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1830.39562435143548946No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1780.38481494292632307No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1770.3826530612244898No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1680.36319612590799033No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1670.36103424420615704No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1650.3567104808024905No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1600.3459010722933241No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1580.34157730888965754No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1570.3394154271878243No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1570.3394154271878243No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1550.3350916637841578No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1550.3350916637841578No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1490.3221203735731581No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1470.31779661016949157No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1470.31779661016949157No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1470.31779661016949157No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1440.3113109650639917No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1410.3048253199584919No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1340.2896921480456589No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1310.28320650294015914No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1290.27888273953649256No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1280.2767208578346593No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1270.274558976132826No Hit
ATCTCGCGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1270.274558976132826No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1250.27023521272915946No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1250.27023521272915946No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1240.26807333102732617No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1230.26591144932549293No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1230.26591144932549293No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1220.26374956762365964No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1190.2572639225181598No Hit
ATCTCGCGGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1170.25294015911449325No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1170.25294015911449325No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1140.24645451400899343No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1100.2378069872016603No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1080.23348322379799377No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1060.22915946039432722No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1030.2226738152888274No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA990.2140262884814943No Hit
ATCTCGCGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT970.20970252507782772No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA960.20754064337599448No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA960.20754064337599448No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA960.20754064337599448No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT940.20321687997232793No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT930.20105499827049464No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC930.20105499827049464No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT920.19889311656866135No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT890.19240747146316153No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC880.19024558976132827No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC870.18808370805949498No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC830.17943618125216187No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC830.17943618125216187No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.1772742995503286No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA810.17511241784849532No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA790.17078865444482877No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT790.17078865444482877No Hit
ATCTCGCGGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT780.1686267727429955No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT780.1686267727429955No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT750.16214112763749566No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT740.1599792459356624No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT730.15781736423382914No Hit
ATCTCGCGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT720.15565548253199585No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA710.15349360083016256No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA710.15349360083016256No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG700.1513317191283293No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT690.14916983742649603No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC680.14700795572466274No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT680.14700795572466274No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC670.14484607402282945No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA660.1426841923209962No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA630.13619854721549635No Hit
ATCTCGCGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT620.13403666551366308No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA620.13403666551366308No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC610.13187478381182982No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA610.13187478381182982No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA610.13187478381182982No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.13187478381182982No Hit
ATCTCGCGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT600.12971290210999656No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG590.12755102040816327No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC590.12755102040816327No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA580.12538913870632998No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT570.12322725700449672No Hit
ATCTCGCGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT570.12322725700449672No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA550.11890349360083015No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.11890349360083015No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT550.11890349360083015No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC540.11674161189899689No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT540.11674161189899689No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA540.11674161189899689No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT540.11674161189899689No Hit
ATCTCGCGGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT530.11457973019716361No Hit
ATCTCGCGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT530.11457973019716361No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.11025596679349707No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.11025596679349707No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA510.11025596679349707No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC500.10809408509166378No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT500.10809408509166378No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA480.10377032168799724No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT470.10160843998616396No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT470.10160843998616396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)