FastQCFastQC Report
Sun 21 May 2023
EGAF00007837919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52957
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG14612.7588420794229283No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT14362.7116339671809206No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG13482.5454614120890535No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12022.269766036595729No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11782.2244462488434014No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT11242.1224767264006648No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9811.852446324376381No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA9661.8241214570311761No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8891.6787204713257924No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8701.6428423060218669No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8351.5767509488830562No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8101.5295428366410484No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7931.4974413203164831No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT7851.4823347243990406No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7331.3841418509356649No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6921.3067205468587724No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6681.261400759106445No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6291.1877561040089128No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6191.1688728591121098No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5651.0669033366693732No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5531.0442434427932097No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5461.0310251713654475No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4840.9139490530052684No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4760.898842457087826No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA4600.868629265252941No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4440.8384160734180561No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT4310.8138678550522123No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA4050.7647714183205242No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3890.7345582264856393No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3850.727004928526918No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3780.7137866570991559No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3730.7043450346507544No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3610.6816851407745907No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3560.6722435183261891No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3540.6684668693468285No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3450.6514719489397058No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA3390.640142002001624No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3370.6363653530222634No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3320.6269237305738617No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3060.5778272938421738No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3030.5721623203731329No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3010.5683856713937724No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2980.5627206979247313No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2920.5513907509866496No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2860.5400608040485677No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA2810.5306191816001662No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC2770.523065883641445No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT2660.5022943142549615No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2650.5004059897652813No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2620.49474101629624034No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2530.47774609588911754No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2420.4569745265026342No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2390.4513095530335933No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2330.43997960609551146No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2320.4380912816058311No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2290.4324263081367902No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2280.4305379836471099No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2220.41920803670902806No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2180.41165473875030684No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2170.40976641426062654No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2050.38710652038446286No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2030.3833298714051023No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1980.37388824895670075No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1900.35878165303925824No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1890.35689332854957795No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1790.3380100836527749No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1780.33612175916309456No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1780.33612175916309456No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1770.33423343467341426No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1750.3304567856940537No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1740.32856846120437333No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1720.32479181222501274No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1700.3210151632456521No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1670.3153501897766112No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1610.3040202428385294No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1590.30024359385916877No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1550.2926902959004475No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1530.2889136469210869No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1530.2889136469210869No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1510.2851369979417263No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1490.28136034896236567No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1470.2775836999830051No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1470.2775836999830051No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1430.27003040202428386No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1430.27003040202428386No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1420.26814207753460356No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1420.26814207753460356No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1410.2662537530449232No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1400.2643654285552429No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1370.258700455086202No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1260.23792888569971865No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1260.23792888569971865No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1240.23415223672035804No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1220.23037558774099742No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1220.23037558774099742No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1190.2247106142719565No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1180.2228222897822762No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1170.22093396529259587No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1160.21904564080291555No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1160.21904564080291555No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1160.21904564080291555No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1140.21526899182355494No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1130.21338066733387465No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1090.20582736937515342No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1080.2039390448854731No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC1020.19260909794739128No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1010.190720773457711No Hit
ATCTCGCGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.18883244896803067No Hit
ATCTCGCGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT990.18694412447835038No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT980.18505579998867006No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA950.17939082651962912No Hit
ATCTCGCGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT940.17750250202994883No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC940.17750250202994883No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC930.1756141775402685No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT930.1756141775402685No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT920.1737258530505882No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT910.1718375285609079No Hit
ATCTCGCGGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT910.1718375285609079No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT910.1718375285609079No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA900.1699492040712276No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG870.16428423060218666No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT850.16050758162282605No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA840.15861925713314576No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT820.15484260815378514No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA800.15106595917442453No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA790.14917763468474424No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC790.14917763468474424No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA770.14540098570538362No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC770.14540098570538362No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA750.141624336726023No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG750.141624336726023No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA740.1397360122363427No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA730.1378476877466624No Hit
ATCTCGCGGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT720.13595936325698207No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA720.13595936325698207No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC700.13218271427762146No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT690.13029438978794117No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC680.12840606529826085No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA680.12840606529826085No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA680.12840606529826085No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.12651774080858055No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT670.12651774080858055No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC670.12651774080858055No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT660.12462941631890025No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT650.12274109182921994No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC650.12274109182921994No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG620.11707611836017902No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT620.11707611836017902No Hit
ATCTCGCGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT620.11707611836017902No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.11518779387049871No Hit
ATCTCGCGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT600.1132994693808184No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC600.1132994693808184No Hit
ATCTCGCGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT600.1132994693808184No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC600.1132994693808184No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC590.1114111448911381No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA590.1114111448911381No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT570.10763449591177747No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.10763449591177747No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA570.10763449591177747No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT560.10574617142209716No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA560.10574617142209716No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA550.10385784693241686No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTATTTGCTCCCCACGCT550.10385784693241686No Hit
ATCTCGCGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT530.10008119795305624No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT530.10008119795305624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)