FastQCFastQC Report
Sun 21 May 2023
EGAF00007837926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61218
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT21223.4663007612140224No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT15892.595641804697965No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA15072.4616942729262634No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14752.4094220654055998No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG13862.2640399882387534No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG13752.246071416903525No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG13632.226469339083276No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG13412.19053219641282No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11181.8262602502531935No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10821.7674540167924464No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT10501.715181809271783No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9941.623705446110621No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9731.5894018099251852No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9681.5812342775000816No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9591.566532719134895No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG9381.5322290829494594No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT8061.316606226926721No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT7451.2169623313404554No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7401.2087947989153518No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7241.1826586951550198No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA6511.0634127217485052No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6341.0356431115031528No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA6281.0258420725930282No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6161.0062399947727791No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5870.9588683067071776No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5670.9261981770067627No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT5610.9163971380966381No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5410.8837270083962232No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5260.8592244111209121No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5260.8592244111209121No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4790.7824496063249371No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4650.7595805155346467No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4560.74487895716946No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4500.7350779182593354No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4300.7024077885589205No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA4180.6828057107386716No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT4140.6762716847985887No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4100.6697376588585057No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3840.6272664902479663No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3780.6174654513378418No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3740.6109314253977588No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3720.6076644124277174No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3650.5962298670325722No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3600.5880623346074685No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3390.5537586984220327No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3320.5423241530268875No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC3180.519455062236597No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3170.5178215557515764No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3130.5112875298114933No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3020.49331895847626517No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2950.48188441308111996No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2880.4704498676859747No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2870.468816361200954No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2800.4573818158058088No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2640.4312457120454768No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2590.4230781796203731No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2480.4051096082851449No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2470.40347610180012417No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2460.40184259531510336No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2240.36590545264464697No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2240.36590545264464697No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2210.36100493318958476No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2080.3397693488843151No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2080.3397693488843151No Hit
ATCTCGCGGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2060.3365023359142736No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2060.3365023359142736No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2000.3267012970041491No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1970.32180077754908687No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1970.32180077754908687No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1940.3169002580940246No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1860.3038322062138587No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1860.3038322062138587No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1830.29893168675879644No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1820.2972981802737757No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1810.29566467378875494No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1750.2858636348786305No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1750.2858636348786305No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1700.27769610245352677No Hit
ATCTCGCGGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1650.269528570028423No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.26299454408834005No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1600.2613610376033193No Hit
ATCTCGCGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1580.2580940246332778No Hit
ATCTCGCGGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1530.24992649220817406No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1470.2401254532980496No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1440.23522493384298734No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1420.23195792087294587No Hit
ATCTCGCGGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1410.2303244143879251No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1400.2286909079029044No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1400.2286909079029044No Hit
ATCTCGCGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.22379038844784213No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1360.2221568819628214No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1350.22052337547780065No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1340.21888986899277987No Hit
ATCTCGCGGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1340.21888986899277987No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1320.2156228560227384No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1300.21235584305269695No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1300.21235584305269695No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1280.2090888300826554No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1240.20255480414257246No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1190.19438727171746872No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1190.19438727171746872No Hit
ATCTCGCGGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1170.19112025874742722No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1160.1894867522624065No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT1160.1894867522624065No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1140.186219739292365No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1120.18295272632232348No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1110.18131921983730276No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1070.17478519389721978No Hit
ATCTCGCGGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1060.17315168741219902No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1060.17315168741219902No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC1050.1715181809271783No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1020.16661766147211604No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA980.16008363553203306No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA950.15518311607697083No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA930.15191610310692935No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA930.15191610310692935No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG930.15191610310692935No Hit
ATCTCGCGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT920.15028259662190857No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA910.14864909013688785No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT900.14701558365186712No Hit
ATCTCGCGGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT890.14538207716684634No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT890.14538207716684634No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT890.14538207716684634No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT880.14374857068182562No Hit
ATCTCGCGGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT860.1404815577117841No Hit
ATCTCGCGGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC840.13721454474174263No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT830.13558103825672188No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG820.13394753177170113No Hit
ATCTCGCGGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT810.13231402528668038No Hit
ATCTCGCGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT810.13231402528668038No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT800.13068051880165965No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA780.12741350583161815No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA780.12741350583161815No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA780.12741350583161815No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA770.1257799993465974No Hit
ATCTCGCGGCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC770.1257799993465974No Hit
ATCTCGCGGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT770.1257799993465974No Hit
ATCTCGCGGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT760.12414649286157665No Hit
ATCTCGCGGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT750.12251298637655592No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA750.12251298637655592No Hit
ATCTCGCGGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT740.12087947989153516No Hit
ATCTCGCGGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC740.12087947989153516No Hit
ATCTCGCGGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.11761246692149367No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA710.11597896043647293No Hit
ATCTCGCGGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC690.11271194746643144No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG690.11271194746643144No Hit
ATCTCGCGGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT690.11271194746643144No Hit
ATCTCGCGGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT670.10944493449638994No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG660.1078114280113692No Hit
ATCTCGCGGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT650.10617792152634847No Hit
ATCTCGCGGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT640.1045444150413277No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA640.1045444150413277No Hit
ATCTCGCGGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC620.10127740207128623No Hit
ATCTCGCGGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.10127740207128623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)