FastQCFastQC Report
Sun 21 May 2023
EGAF00007837938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85912
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT26873.127618958934724No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA24452.8459353757333083No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG24362.835459539994413No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG22182.581711518763386No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT20692.4082782381972248No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA18472.149874289971133No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG16041.8670267250209518No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG15581.8134835645777072No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA14721.713381134183816No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14101.641214265760313No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT13811.6074587950460937No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG13741.5993109228047304No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG13491.5702113790855758No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT11421.3292671570909769No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA10931.272232051401434No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT10631.2373125989384486No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10581.2314926901946177No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA10341.2035571282242294No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT10091.174457584505075No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG10031.167473694012478No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9821.1430300772883881No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG9571.1139305335692338No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT9431.0976347890865072No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9301.0825030263525468No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA9041.0522395008846261No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8510.9905484682000186No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7700.8962659465499581No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT7520.8753142750721669No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7270.8462147313530124No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7120.8287550051215197No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA6950.8089673153924946No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT6520.7589161001955489No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG6440.7496042462054195No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6280.7309805382251606No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6230.7251606294813298No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT6190.720504702486265No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5640.6564857063041252No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG5350.622730235589906No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5130.59712263711705No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT5120.5959586553682839No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4870.5668591116491294No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4850.564531148151597No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4730.5505633671664029No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT4620.5377595679299748No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4600.5354316044324425No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4580.5331036409349101No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT4350.506332060713288No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT4340.5051680789645219No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT4050.4714126082503026No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC4020.4679206630040041No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3910.45511686376757615No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT3880.45162491852127756No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3770.4388211192848496No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3750.4364931557873173No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3680.428345283545954No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3670.42718130179718783No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3570.415541484309526No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3570.415541484309526No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT3450.4015737033243319No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3450.4015737033243319No Hit
ATCTGCGCACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT3150.36665425086134645No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3140.3654902691125803No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2820.3282428531520626No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA2750.3200949809106993No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2730.317767017413167No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2590.3014712729304404No Hit
ATCTGCGCACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2560.2979793276841419No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2460.28633951019648013No Hit
ATCTGCGCACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT2440.2840115466989478No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2390.27819163795511687No Hit
ATCTGCGCACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2380.27702765620635067No Hit
ATCTGCGCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2360.27469969270881833No Hit
ATCTGCGCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2350.27353571096005214No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2350.27353571096005214No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2350.27353571096005214No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA2340.27237172921128594No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2260.26305987522115654No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2220.2584039482260918No Hit
ATCTGCGCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2220.2584039482260918No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT2170.25258403948226094No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT2130.2479281124871962No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2100.24443616724089764No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2080.2421082037433653No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2050.23861625849706677No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT2030.23628829499953444No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1950.226976441009405No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1890.21999255051680788No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1890.21999255051680788No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1870.21766458701927555No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1800.20951671477791228No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1780.20718875128037992No Hit
ATCTGCGCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1780.20718875128037992No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1750.2036968060340814No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1740.2025328242853152No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT1720.20020486078778285No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1710.19904087903901668No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC1710.19904087903901668No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1700.1978768972902505No Hit
ATCTGCGCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1680.19554893379271815No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1670.19438495204395195No Hit
ATCTGCGCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1670.19438495204395195No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1640.19089300679765342No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1630.18972902504888722No Hit
ATCTGCGCACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.18740106155135489No Hit
ATCTGCGCACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1600.1862370798025887No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1580.18390911630505635No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1580.18390911630505635No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT1510.1757612440636931No Hit
ATCTGCGCACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1510.1757612440636931No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1510.1757612440636931No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1500.17459726231492692No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1500.17459726231492692No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT1460.1699413353198622No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1440.16761337182232983No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1410.1641214265760313No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT1410.1641214265760313No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1400.16295744482726512No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1400.16295744482726512No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1360.1583015178322004No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1360.1583015178322004No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1310.1524816090883695No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1280.14898966384207096No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1270.14782568209330477No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1260.1466617003445386No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1250.1454977185957724No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1240.14433373684700623No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1220.1420057733494739No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1210.1408417916007077No Hit
ATCTGCGCACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.13851382810317533No Hit
ATCTGCGCACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1190.13851382810317533No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT1170.13618586460564297No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT1170.13618586460564297No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1150.13385790110811063No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1130.13152993761057827No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1110.1292019741130459No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1110.1292019741130459No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT1110.1292019741130459No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1110.1292019741130459No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC1100.12803799236427973No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1070.12454604711798119No Hit
ATCTGCGCACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1070.12454604711798119No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCT1060.12338206536921502No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1050.12221808362044882No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1040.12105410187168265No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT1040.12105410187168265No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1030.11989012012291647No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1020.1187261383741503No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT970.11290622963031939No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA960.11174224788155322No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT940.10941428438402086No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA930.10825030263525467No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT920.1070863208864885No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA910.10592233913772231No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG900.10475835738895614No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT900.10475835738895614No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTG900.10475835738895614No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC890.10359437564018996No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC880.10243039389142379No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC880.10243039389142379No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA870.1012664121426576No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT860.10010243039389143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)