FastQCFastQC Report
Sun 21 May 2023
EGAF00007837940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52223
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA30395.81927503207399No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA29025.556938513681711No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT21424.101641039388775No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14582.7918733125251323No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14242.726767899201501No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11882.2748597361315896No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT11152.1350745839955576No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT10201.9531623997089405No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9961.9072056373628479No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9631.8440150891369702No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7941.5204028876165674No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT7821.4974245064435212No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7621.4591272044884438No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7121.3633839496007505No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT6031.1546636539455795No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5671.0857285104264405No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5471.047431208471363No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT5361.0263676923960707No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT5271.0091339065162859No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5231.0014744461252705No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4870.9325393026061314No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4680.8961568657488079No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4480.8578595637937306No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4290.8214771269364074No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4180.8004136108611148No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4130.7908392853723455No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3930.7525419834172683No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3860.7391379277329913No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3770.7219041418532065No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3700.7085000861689293No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3670.7027554908756678No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3630.6950960304846524No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3590.6874365700936369No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3560.6816919748003752No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3450.6606284587250828No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3320.6357352124542825No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT3090.5916933152059437No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2940.5629703387396358No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2830.5419068226643433No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2730.5227581716868047No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2700.5170135763935431No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2650.5074392509047737No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2530.48446086973172736No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2510.48063113953621966No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2400.4595676234609272No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2360.4519081630699117No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT2250.4308446469946193No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2230.4270149167991115No Hit
ATCTGCGCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2220.4251000517013577No Hit
ATCTGCGCACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2210.4231851866036038No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2110.4040365356260652No Hit
ATCTGCGCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2110.4040365356260652No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2100.40212167052831127No Hit
ATCTGCGCACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT2060.3944622101372959No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2060.3944622101372959No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2040.39063247994178807No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1920.3676540987687417No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1900.363824368573234No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1890.3619095034754802No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1870.3580797732799724No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1830.350420312888957No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1820.3485054477912031No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1790.3427608524979415No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1780.3408459874001876No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1770.33893112230243383No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1760.3370162572046799No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1600.30637841564061813No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1580.3025486854451104No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1550.2968040901518488No Hit
ATCTGCGCACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1530.2929743599563411No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1520.2910594948585872No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1500.2872297646630795No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1470.2814851693698179No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1430.27382570897880243No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1430.27382570897880243No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1400.26808111368554083No Hit
ATCTGCGCACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1400.26808111368554083No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1390.266166248587787No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1370.2623365183922793No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1310.2508473278057561No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1300.24893246270800223No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1290.24701759761024833No Hit
ATCTGCGCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1190.2278689466327097No Hit
ATCTGCGCACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.22595408153495586No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1170.22403921643720198No Hit
ATCTGCGCACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1150.22020948624169429No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1140.2182946211439404No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1140.2182946211439404No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1060.2029757003619095No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1060.2029757003619095No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1060.2029757003619095No Hit
ATCTGCGCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1040.1991459701664018No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT1030.19723110506864794No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1030.19723110506864794No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1020.19531623997089403No Hit
ATCTGCGCACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1010.19340137487314019No Hit
ATCTGCGCACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT950.181912184286617No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA940.17999731918886314No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA910.17425272389560156No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT900.1723378587978477No Hit
ATCTGCGCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT900.1723378587978477No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA880.16850812860233996No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC880.16850812860233996No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT870.16659326350458611No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC870.16659326350458611No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA870.16659326350458611No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA870.16659326350458611No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC850.1627635333090784No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT840.1608486682113245No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC840.1608486682113245No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC820.1570189380158168No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA810.1551040729180629No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA800.15318920782030906No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT780.14935947762480134No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT780.14935947762480134No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA780.14935947762480134No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC780.14935947762480134No Hit
ATCTGCGCACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT760.1455297474292936No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT750.14361488233153974No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT730.13978515213603201No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA730.13978515213603201No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA730.13978515213603201No Hit
ATCTGCGCACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT720.13787028703827817No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA720.13787028703827817No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA710.1359554219405243No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA710.1359554219405243No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.13404055684277041No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT690.13212569174501657No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT680.1302108266472627No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA680.1302108266472627No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC670.12829596154950884No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT670.12829596154950884No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA650.12446623135400112No Hit
ATCTGCGCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT650.12446623135400112No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT640.12255136625624724No Hit
ATCTGCGCACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT620.11872163606073952No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT620.11872163606073952No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT600.1148919058652318No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.11297704076747793No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT590.11297704076747793No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC590.11297704076747793No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA570.1091473105719702No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT560.10723244547421633No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA560.10723244547421633No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.10723244547421633No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCT540.10340271527870862No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.10148785018095476No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA530.10148785018095476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)