FastQCFastQC Report
Sun 21 May 2023
EGAF00007837968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42354
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA10582.4979931057279123No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9172.1650847617698443No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA9142.158001605515418No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA8331.9667563866458893No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8271.9525900741370357No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7691.815649053218114No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7451.7589838031826983No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA6671.574821740567597No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6181.4591301884119563No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6171.4567691363271473No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5941.4024649383765404No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5921.3977428342069225No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5731.3528828445955516No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5611.324550219577844No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5481.293856542475327No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5381.270246021627237No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5291.2489965528639562No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5261.2419133966095293No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5101.2041365632525853No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5031.1876091986589223No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4971.1734428861500685No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4881.1521934173867876No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4511.064834490248855No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4401.038862917315956No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4291.0128913443830572No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4261.00580818812863No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4200.9916418756197762No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4140.9774755631109222No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4100.9680313547716862No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3980.9396987297539784No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3760.8877555838881807No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3640.8594229588704727No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3620.8547008547008548No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3500.8263682296831468No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3400.8027577088350569No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3380.798035604665439No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3360.7933135004958211No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3340.7885913963262029No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3100.7319261462907871No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2970.7012324691882702No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2930.6917882608490343No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2810.6634556358313264No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2580.6091514378807197No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2480.5855409170326297No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2380.5619303961845399No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2340.5524861878453038No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2240.528875666997214No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2220.5241535628275961No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2180.51470935448836No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2110.4981819898946971No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2060.48637672947065214No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2030.4792935732162252No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2010.47457146904660713No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1800.4249893752656183No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1790.42262832318080934No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1750.4131841148415734No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1750.4131841148415734No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1740.4108230627567644No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1740.4108230627567644No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1720.4061009585871464No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1700.40137885441752846No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1680.39665675024791053No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1640.3872125419086745No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1640.3872125419086745No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1630.3848514898238655No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1570.37068517731501155No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1530.3612409689757756No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1520.35887991689096665No Hit
TACCTCGTACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1430.3376304481276857No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1300.3069367710251688No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1270.29985361477074185No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1210.2856873022618879No Hit
TACCTCGTACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.2738820418378429No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1160.2738820418378429No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1160.2738820418378429No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1160.2738820418378429No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1150.27152098975303396No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1110.26207678141379803No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1100.259715729328989No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1090.25735467724418No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1060.250271520989753No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1040.24554941682013504No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1020.24082731265051707No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1010.2384662605657081No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG950.22429994805685413No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT940.22193889597204516No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA910.21485573971761818No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA900.21249468763280915No Hit
TACCTCGTACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT900.21249468763280915No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT900.21249468763280915No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT850.20068942720876423No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC830.19596732303914624No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA810.19124521886952825No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC800.18888416678471928No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA790.1865231146999103No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.1841620626151013No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA780.1841620626151013No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA780.1841620626151013No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA760.17943995844548333No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC760.17943995844548333No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT750.1770789063606743No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA750.1770789063606743No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC750.1770789063606743No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.1747178542758653No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA720.16999575010624735No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA720.16999575010624735No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC710.16763469802143835No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.16527364593662935No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA690.16291259385182036No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC690.16291259385182036No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA690.16291259385182036No Hit
TACCTCGTACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT670.1581904896822024No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA660.1558294375973934No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA650.1534683855125844No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA650.1534683855125844No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT640.1511073334277754No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.14874628134296644No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA620.14638522925815742No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA610.14402417717334845No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA610.14402417717334845No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA600.14166312508853945No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC600.14166312508853945No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA600.14166312508853945No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA600.14166312508853945No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT590.13930207300373046No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC580.13694102091892146No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA570.13457996883411247No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC570.13457996883411247No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA570.13457996883411247No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA560.1322189167493035No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA560.1322189167493035No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC550.1298578646644945No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA550.1298578646644945No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT550.1298578646644945No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA540.1274968125796855No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA530.1251357604948765No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT530.1251357604948765No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA530.1251357604948765No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.12277470841006752No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT520.12277470841006752No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.12041365632525854No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT510.12041365632525854No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA510.12041365632525854No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA510.12041365632525854No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC500.11805260424044954No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA500.11805260424044954No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA500.11805260424044954No Hit
TACCTCGTACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT500.11805260424044954No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC490.11569155215564056No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT490.11569155215564056No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC490.11569155215564056No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC470.11096944798602258No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC470.11096944798602258No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT470.11096944798602258No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT460.10860839590121359No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA460.10860839590121359No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC450.10624734381640458No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT450.10624734381640458No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC440.10388629173159561No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA440.10388629173159561No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA430.1015252396467866No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA430.1015252396467866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)