FastQCFastQC Report
Sun 21 May 2023
EGAF00007837969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42354
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT12803.0221466685555085No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10862.564102564102564No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA9102.148557397176182No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8762.068281626292676No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA7871.8581479907446758No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA7251.7117627614865183No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6981.6480143551966757No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6621.563016480143552No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6451.522878594701799No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA5931.4001038862917317No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5691.3434386362563158No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5441.2844123341360911No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5281.2466355007791472No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5191.2253860320158663No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5091.2017755111677764No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5031.1876091986589223No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5001.1805260424044954No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4921.1616376257260235No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4911.1592765736412145No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4881.1521934173867876No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4831.1403881569627425No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4791.1309439486235067No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4771.1262218444538885No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4641.0955281673513717No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4471.0553902819096188No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4341.024696604807102No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4321.019974500637484No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4190.9892808235349672No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4080.9633092506020683No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4050.9562260943476413No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4020.9491429380932143No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3970.9373376776691694No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3880.9160882089058884No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3870.9137271568210795No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3810.8995608443122255No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3440.8122019171742928No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3440.8122019171742928No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3210.7578977192236861No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2970.7012324691882702No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2900.6847051045946073No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2860.6752608962553714No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2850.6728998441705625No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2770.6540114274920905No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2610.6162345941351466No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2540.5997072295414837No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2350.5548472399301129No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2290.540680927421259No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2200.519431458657978No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2140.505265146149124No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2130.502904094064315No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2110.4981819898946971No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2050.4840156773858431No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2040.48165462530103414No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2020.4769325211314162No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1900.44859989611370826No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1880.4438777919440903No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1820.42971147943523635No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1770.4179062190111914No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1730.4084620106719554No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1720.4061009585871464No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1660.3919346460782925No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1620.3824904377390565No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1610.38012938565424753No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1610.38012938565424753No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1590.3754072814846296No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1540.3636020210605846No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1480.3494357085517306No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1400.3305472918732587No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1350.3187420314492138No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1330.31401992727959577No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1320.3116588751947868No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1310.3092978231099778No Hit
TACCTCGTACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.29040940643150587No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1190.28096519809226994No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1180.2786041460074609No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1180.2786041460074609No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1170.2762430939226519No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1110.26207678141379803No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1090.25735467724418No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1090.25735467724418No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1090.25735467724418No Hit
TACCTCGTACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1020.24082731265051707No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1000.23610520848089908No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG980.23138310431128112No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT950.22429994805685413No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA940.22193889597204516No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC930.21957784388723614No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG920.21721679180242717No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA870.2054115313783822No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT870.2054115313783822No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA840.19832837512395526No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA830.19596732303914624No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT830.19596732303914624No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT830.19596732303914624No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA820.19360627095433725No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC810.19124521886952825No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG780.1841620626151013No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA770.1818010105302923No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC740.1747178542758653No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA740.1747178542758653No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA740.1747178542758653No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG730.17235680219105634No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC730.17235680219105634No Hit
TACCTCGTACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT710.16763469802143835No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA710.16763469802143835No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.16763469802143835No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT680.1605515417670114No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA670.1581904896822024No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA670.1581904896822024No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA670.1581904896822024No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT660.1558294375973934No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA650.1534683855125844No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA640.1511073334277754No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA630.14874628134296644No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA630.14874628134296644No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA620.14638522925815742No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA610.14402417717334845No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT610.14402417717334845No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA610.14402417717334845No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT600.14166312508853945No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT600.14166312508853945No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT600.14166312508853945No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA590.13930207300373046No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA580.13694102091892146No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT570.13457996883411247No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA570.13457996883411247No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA560.1322189167493035No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA550.1298578646644945No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT550.1298578646644945No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA550.1298578646644945No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA540.1274968125796855No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA540.1274968125796855No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT520.12277470841006752No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA510.12041365632525854No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA510.12041365632525854No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC510.12041365632525854No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA510.12041365632525854No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT500.11805260424044954No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT490.11569155215564056No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA480.11333050007083156No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT480.11333050007083156No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC480.11333050007083156No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC480.11333050007083156No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT480.11333050007083156No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC480.11333050007083156No Hit
TACCTCGTACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT470.11096944798602258No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC470.11096944798602258No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA470.11096944798602258No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.11096944798602258No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.11096944798602258No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA460.10860839590121359No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT460.10860839590121359No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTG450.10624734381640458No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC450.10624734381640458No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT440.10388629173159561No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC440.10388629173159561No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT440.10388629173159561No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT430.1015252396467866No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT430.1015252396467866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)