FastQCFastQC Report
Sun 21 May 2023
EGAF00007837981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60410
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT29014.802185068697236No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA27604.56878000331071No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA25604.23770898857805No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT16072.6601556033769245No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA11951.9781493130276444No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA11211.85565303757656No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT11211.85565303757656No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT11061.830822711471611No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA10851.7960602549246814No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA10251.696738950504883No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9011.491474921370634No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8941.479887435854991No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8731.4451249793080616No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8691.4385035590134083No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7661.2680019864260883No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7281.2050984936268831No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT7191.1902002979639132No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6361.0528058268498592No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6231.0312862108922363No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT5940.9832809137560007No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5880.9733487833140209No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5800.9601059427247144No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT5710.9452077470617447No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5630.9319649064724383No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4910.8127793411686808No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4640.7680847541797715No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4560.7548419135904652No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4500.7449097831484853No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA4400.7283562324118523No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA4350.7200794570435358No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4140.6853170004966065No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3990.660486674391657No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3970.6571759642443304No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3830.6340009932130442No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3660.6058599569607681No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT3540.5859956960768085No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3540.5859956960768085No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3480.5760635656348286No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3190.5280582684985929No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3020.49991723224631684No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2980.49329581195166367No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCT2960.489985101804337No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2900.4800529713623572No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2900.4800529713623572No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2850.47177619599404075No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2850.47177619599404075No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2850.47177619599404075No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2720.4502565800364178No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2720.4502565800364178No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT2700.4469458698890912No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2670.44197980466810133No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2530.4188048336368151No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2500.41383876841582523No Hit
ATCTGCGCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2480.41052805826849853No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2480.41052805826849853No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2390.3956298626055289No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT2360.390663797384539No Hit
ATCTGCGCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2260.374110246647906No Hit
ATCTGCGCACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2250.37245489157424266No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2250.37245489157424266No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2200.36417811620592616No Hit
ATCTGCGCACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2160.357556695911273No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2160.357556695911273No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2100.34762456546929316No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2090.3459692103956299No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT2070.34265850024830324No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2050.33934779010097665No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1980.32776030458533356No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1870.3095513987750373No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1800.29796391325939414No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1800.29796391325939414No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1800.29796391325939414No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTG1790.2963085581857308No Hit
ATCTGCGCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1760.29134249296474096No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1750.28968713789107764No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCT1720.2847210726700877No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1710.28306571759642446No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTG1700.28141036252276114No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1640.2714782320807813No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1560.25823539149147495No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCT1560.25823539149147495No Hit
ATCTGCGCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1510.24995861612315842No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1480.2449925509021685No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1470.24333719582850522No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG1430.236715775533852No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1420.2350604204601887No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1390.23009435523919883No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT1380.2284390001655355No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1330.220162224797219No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1290.21354080450256582No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1280.2118854494289025No Hit
ATCTGCGCACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1250.20691938420791262No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG1190.1969872537659328No Hit
ATCTGCGCACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1190.1969872537659328No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1180.1953318986922695No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1170.1936765436186062No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.19202118854494288No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1140.1887104783976163No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1120.1853997682502897No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1120.1853997682502897No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1120.1853997682502897No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1100.18208905810296308No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1100.18208905810296308No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA1050.17381228273464658No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1050.17381228273464658No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1040.17215692766098328No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1000.16553550736633008No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT990.16388015229266678No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA990.16388015229266678No Hit
ATCTGCGCACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT970.16056944214534016No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT930.15394802185068696No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA920.15229266677702366No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA900.14898195662969707No Hit
ATCTGCGCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT900.14898195662969707No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA890.14732660155603378No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCATGCT880.14567124648237048No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTG870.14401589140870716No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT850.14070518126138057No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC840.13904982618771727No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC840.13904982618771727No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT830.13739447111405398No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCT830.13739447111405398No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.13573911604039066No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT820.13573911604039066No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT800.13242840589306407No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG780.12911769574573748No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT780.12911769574573748No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA770.12746234067207415No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCT760.12580698559841086No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCGCACC750.12415163052474755No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCT750.12415163052474755No Hit
ATCTGCGCACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT740.12249627545108425No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA730.12084092037742096No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCT720.11918556530375767No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC710.11753021023009436No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC710.11753021023009436No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG710.11753021023009436No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT710.11753021023009436No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA700.11587485515643105No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.11421950008276775No Hit
ATCTGCGCACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT690.11421950008276775No Hit
ATCTGCGCACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT690.11421950008276775No Hit
ATCTGCGCACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT690.11421950008276775No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA680.11256414500910444No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA670.11090878993544116No Hit
ATCTGCGCACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT660.10925343486177785No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC660.10925343486177785No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA650.10759807978811456No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTG650.10759807978811456No Hit
ATCTGCGCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT650.10759807978811456No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT640.10594272471445125No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG640.10594272471445125No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG640.10594272471445125No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC640.10594272471445125No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT630.10428736964078794No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC630.10428736964078794No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCATGCT610.10097665949346135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)