FastQCFastQC Report
Sun 21 May 2023
EGAF00007837982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007837982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77384
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT28363.6648402770598576No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA26713.4516179055101825No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA24413.154398842137909No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG20422.6387883800268788No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA20092.596143905716944No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG19452.513439470691616No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT18742.421689238085392No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA18322.3674144526000207No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA18182.34932285743823No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA17162.2175126641166134No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT14101.8220820841517624No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG12411.603690685413005No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT11721.5145249664013232No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG11081.431820531375995No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT9811.2677039181226093No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9791.2651194045280678No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9641.2457355525690066No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT8151.0531892897756643No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT7981.0312209242220614No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7650.9885764499121266No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7110.9187945828595059No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6880.8890726765222784No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6710.8671043109686758No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6410.828336607050553No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT6260.8089527550914917No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5780.7469244288224955No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5730.7404631448361418No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA5690.7352941176470588No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT5660.7314173472552465No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA5640.7288328336607051No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5640.7288328336607051No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5550.7172025224852683No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5530.7146180088907268No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5490.7094489817016438No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5000.6461283986353769No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4990.644836141838106No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4950.639667114649023No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTA4890.6319135738653985No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4860.6280368034735863No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4660.6021916675281712No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTA4580.5918536131500052No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA4130.5337020572728213No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4050.5233640028946552No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3780.4884730693683449No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3760.4858885557738034No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA3750.48459629897653256No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT3620.46779696061201287No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3570.461335676625659No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3490.45099762224749307No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3480.4497053654502222No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3410.44065956786932703No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3400.4393673110720563No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3360.43419828388297327No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3360.43419828388297327No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT3280.42386022950480723No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3180.4109376615320997No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3160.4083531479375581No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3110.40189186395120435No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2990.38638478238395535No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2930.3786312416003308No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2830.36570867362762327No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2710.3502015920603742No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT2670.3450325648712912No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2650.3424480512767497No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTATTTGCTCCCCACACT2600.33598676729039595No Hit
ATCTGCGCACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2570.33210999689858367No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2560.33081774010131293No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT2540.3282332265067714No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG2520.3256487129122299No Hit
ATCTGCGCACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2510.32435645611495917No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2510.32435645611495917No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT2370.3062648609531686No Hit
ATCTGCGCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2330.3010958337640856No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG2320.2998035769668148No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA2320.2998035769668148No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA2300.29721906337227333No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA2300.29721906337227333No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT2290.2959268065750026No Hit
ATCTGCGCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2210.28558875219683655No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT2180.28171198180502427No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA2100.2713739274268583No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2050.26491264344050447No Hit
ATCTGCGCACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2030.262328129845963No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1870.2416520210896309No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1830.2364829939005479No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1830.2364829939005479No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1820.23519073710327718No Hit
ATCTGCGCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1810.23389848030600643No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1770.22872945311692341No Hit
ATCTGCGCACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1730.2235604259278404No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT1680.21709914194148663No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1680.21709914194148663No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1640.21193011475240361No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCC1630.21063785795513282No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1610.20805334436059136No Hit
ATCTGCGCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.20805334436059136No Hit
ATCTGCGCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1600.20676108756332057No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1530.19771528998242532No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACACT1500.19383851959061305No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1480.19125400599607154No Hit
ATCTGCGCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.18608497880698854No Hit
ATCTGCGCACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1390.17962369482063476No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1370.17703918122609325No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1350.17445466763155176No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTA1340.173162410834281No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1340.173162410834281No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTA1340.173162410834281No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1320.17057789723973948No Hit
ATCTGCGCACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1310.16928564044246872No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1250.1615320996588442No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1200.15507081567249043No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1160.1499017884834074No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGATACCCACGCT1150.14860953168613666No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1150.14860953168613666No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1140.14731727488886592No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCT1110.14344050449705364No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT1090.14085599090251213No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1080.1395637341052414No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCC1080.1395637341052414No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT1050.13568696371342914No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1050.13568696371342914No Hit
ATCTGCGCACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1040.13439470691615837No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTATTTGCTCCCCACACT1030.13310245011888763No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1020.13181019332161686No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT1010.13051793652434612No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1000.12922567972707535No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT950.12276439574072161No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA950.12276439574072161No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC950.12276439574072161No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC940.12147213894345085No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTATTTGCTCCCCACACT940.12147213894345085No Hit
ATCTGCGCACCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT910.11759536855163859No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT910.11759536855163859No Hit
ATCTGCGCACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT890.11501085495709706No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTATTTGCTCCCCACACT870.11242634136255555No Hit
ATCTGCGCACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT870.11242634136255555No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG870.11242634136255555No Hit
ATCTGCGCACCGGACTACCCGGGTATCTAATCCTGTTTGATACCCACGCT860.1111340845652848No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA850.10984182776801407No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT840.10854957097074332No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT830.10725731417347256No Hit
ATCTGCGCACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT820.10596505737620181No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT810.10467280057893105No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCC810.10467280057893105No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA800.10338054378166028No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.10338054378166028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)