FastQCFastQC Report
Sun 21 May 2023
EGAF00007838001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46303
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17493.7772930479666544No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16713.6088374403386387No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT14213.0689156210180766No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA12442.6866509729391184No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA11222.423169125110684No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT10242.2115197719370236No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7911.7083126363302594No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7751.6737576398937433No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7521.6240848325162518No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7511.6219251452389696No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6471.3973176684016155No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6221.3433254864695592No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5941.2828542427056562No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5891.2720558063192449No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT5811.2547783081009871No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5801.2526186208237047No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5681.2267023734963178No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5361.1575923806232857No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5021.0841630131956892No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4610.995615834827117No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4500.9718592747770124No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4460.9632205256678832No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC4460.9632205256678832No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4060.8768330345765932No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4010.866034598190182No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3590.7753277325443276No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3440.7429324233850939No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3380.7299742997214004No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3310.7148564887804245No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC3190.6889402414530377No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3160.6824611796211908No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3120.6738224305120618No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3080.6651836814029328No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3040.6565449322938038No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2950.6371077467982637No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2780.6003930630844654No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2720.5874349394207719No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2700.5831155648662074No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2700.5831155648662074No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2630.5679977539252317No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2580.5571993175388203No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2530.5464008811524091No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2470.5334427574887156No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2460.5312830702114334No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2360.5096861974386109No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2220.4794505755566594No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2150.4643327646156836No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2140.4621730773384014No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2140.4621730773384014No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC2130.4600133900611192No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2120.4578537027838369No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2110.45569401550655464No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2080.44921495367470793No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2070.44705526639742565No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2000.4319374554564499No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1950.42113901907003864No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1870.4038615208517807No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1790.38658402263352265No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1760.38010496080167594No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1750.37794527352439367No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1740.3757855862471114No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1660.3585080880288534No Hit
ATCTGCGCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1650.35634840075157115No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1620.3498693389197244No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1540.3325918407014664No Hit
ATCTGCGCACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1510.3261127788696197No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC1470.3174740297604907No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1460.31531434248320844No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1420.30667559337407946No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1410.3045159060967972No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1390.3001965315422327No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1370.29587715698766814No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1360.2937174697103859No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1330.28723840787853916No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1310.2829190333239747No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1300.28075934604669245No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1270.2742802842148457No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1260.27212059693756346No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC1250.2699609096602812No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1250.2699609096602812No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1240.2678012223829989No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1180.25484309871930544No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1170.25268341144202316No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1160.25052372416474095No Hit
ATCTGCGCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1150.24836403688745867No Hit
ATCTGCGCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1150.24836403688745867No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1140.24620434961017645No Hit
ATCTGCGCACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1140.24620434961017645No Hit
ATCTGCGCACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1130.2440446623328942No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1120.24188497505561196No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1100.23756560050104744No Hit
ATCTGCGCACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1070.2310865386692007No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1060.22892685139191846No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC1040.22460747683735396No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1040.22460747683735396No Hit
ATCTGCGCACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1020.22028810228278944No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1020.22028810228278944No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1000.21596872772822495No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC990.21380904045094268No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG980.21164935317366046No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC980.21164935317366046No Hit
ATCTGCGCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT980.21164935317366046No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG960.20732997861909594No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA950.2051702913418137No Hit
ATCTGCGCACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT950.2051702913418137No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC940.20301060406453147No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.20085091678724917No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC920.19869122950996695No Hit
ATCTGCGCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT910.1965315422326847No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA900.19437185495540246No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA900.19437185495540246No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA890.1922121676781202No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT890.1922121676781202No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT880.19005248040083797No Hit
ATCTGCGCACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT870.1878927931235557No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA860.18573310584627348No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC840.18141373129170896No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT810.1749346694598622No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA810.1749346694598622No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC810.1749346694598622No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA800.17277498218257997No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA790.1706152949052977No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.16845560762801545No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA750.1619765457961687No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA740.15981685851888647No Hit
ATCTGCGCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT740.15981685851888647No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT740.15981685851888647No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT720.15549748396432198No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA720.15549748396432198No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA720.15549748396432198No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA710.15333779668703973No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA710.15333779668703973No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT700.15117810940975746No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGATACCCGCACC690.14901842213247524No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC690.14901842213247524No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA640.13821998574606398No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC640.13821998574606398No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT630.13606029846878173No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.13606029846878173No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT610.13174092391421724No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA590.12742154935965272No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.12742154935965272No Hit
ATCTGCGCACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT580.12526186208237047No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG580.12526186208237047No Hit
ATCTGCGCACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT560.12094248752780598No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC550.11878280025052372No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.11662311297324147No Hit
ATCTGCGCACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT540.11662311297324147No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC530.11446342569595923No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA530.11446342569595923No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT530.11446342569595923No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.11014405114139472No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA510.11014405114139472No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC510.11014405114139472No Hit
ATCTGCGCACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT500.10798436386411248No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT490.10582467658683023No Hit
ATCTGCGCACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT490.10582467658683023No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACATATGGGGCGAGCGTTA490.10582467658683023No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT480.10366498930954797No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT480.10366498930954797No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA480.10366498930954797No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT480.10366498930954797No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA470.10150530203226574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)