FastQCFastQC Report
Sun 21 May 2023
EGAF00007838004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48018
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA28105.851972177100254No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT19414.042234162189179No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16083.348744220917156No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10652.217918280644758No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT10622.211670623516181No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8821.8368111958015743No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT8651.8014078054063059No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA8041.674372110458578No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7581.5785747011537339No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT7581.5785747011537339No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA7121.4827772918488902No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6991.4557041109583906No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6351.3224207588820858No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6091.268274397101087No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5351.1141655212628598No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5341.1120829688866674No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA4861.012120454829439No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4811.0017076929484776No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4680.9746345120579782No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4610.9600566454246324No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4570.9517264359198634No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA4390.9142404931484027No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA4300.8954975217626723No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4240.8830022075055187No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3890.8101128743387895No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3750.7809571410720979No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3730.7767920363197134No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3390.7059852555291766No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3380.7039027031529843No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3260.6789120746386772No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3140.65392144612437No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA3030.6310133699862551No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA3030.6310133699862551No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT3020.628930817610063No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2970.6185180557291016No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2970.6185180557291016No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2930.6101878462243325No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2870.597692531967179No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2760.5747844558290641No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT2750.5727019034528719No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2680.558124036819526No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2680.558124036819526No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2560.5331334083052188No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2500.5206380940480654No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2480.5164729892956809No Hit
ATCTGCGCACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2290.4769044941480278No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2280.4748219417718355No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT2280.4748219417718355No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2220.462326627514682No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2200.4581615227622975No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2200.4581615227622975No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2150.44774876088133614No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2130.4435836561289516No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2110.43941855137656716No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA2080.4331708942479903No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2060.4290057894956058No Hit
ATCTGCGCACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT2000.4165104752384522No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1990.41442792286226No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1970.41026281810987547No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1950.40609771335749095No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1920.3998500562289142No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1840.38318963721937604No Hit
ATCTGCGCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1830.3811070848431838No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1810.37694198009079927No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1770.36861177058603023No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1710.3561164563288767No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1670.34778624682410764No Hit
ATCTGCGCACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1580.32904327543837725No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1500.3123828564288392No Hit
ATCTGCGCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1490.3103003040526469No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1460.30405264692407014No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1460.30405264692407014No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1400.2915573326669166No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1400.2915573326669166No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1380.28739222791453206No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1370.2853096755383398No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1320.2748969136573785No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1320.2748969136573785No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1290.26864925652880167No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1270.26448415177641715No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1250.2603190470240327No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1250.2603190470240327No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1240.25823649464784043No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1210.2519888375192636No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT1190.24782373276687908No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT1180.24574118039068682No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1170.24365862801449456No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1160.2415760756383023No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1130.2353284185097255No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1130.2353284185097255No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1080.22491565662876423No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1050.21866799950018742No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1040.21658544712399516No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1030.2145028947478029No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1030.2145028947478029No Hit
ATCTGCGCACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1020.21242034237161064No Hit
ATCTGCGCACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1020.21242034237161064No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA950.19784247573826483No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC940.19575992336207257No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA940.19575992336207257No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA920.19159481860968802No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA920.19159481860968802No Hit
ATCTGCGCACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT910.18951226623349576No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA910.18951226623349576No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG890.18534716148111127No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCATGCT890.18534716148111127No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT880.18326460910491899No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT880.18326460910491899No Hit
ATCTGCGCACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT870.18118205672872673No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC860.17909950435253447No Hit
ATCTGCGCACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT840.17493439960014995No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA830.17285184722395766No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCATGCT800.1666041900953809No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT790.16452163771918862No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT780.16243908534299636No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC780.16243908534299636No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA780.16243908534299636No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA770.1603565329668041No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT770.1603565329668041No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC770.1603565329668041No Hit
ATCTGCGCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT760.15827398059061187No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCT760.15827398059061187No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT760.15827398059061187No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT760.15827398059061187No Hit
ATCTGCGCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT720.1499437710858428No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.1499437710858428No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG720.1499437710858428No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA700.1457786663334583No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA700.1457786663334583No Hit
ATCTGCGCACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT690.14369611395726603No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT670.1395310092048815No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA660.13744845682868925No Hit
ATCTGCGCACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT640.1332833520763047No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC630.13120079970011245No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT600.12495314257153567No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT600.12495314257153567No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT600.12495314257153567No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG590.12287059019534341No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC570.11870548544295888No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.11662293306676663No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC560.11662293306676663No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA560.11662293306676663No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCATGCT550.11454038069057437No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT550.11454038069057437No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCT540.11245782831438211No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT530.11037527593818984No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.10829272356199758No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT520.10829272356199758No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT510.10621017118580532No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA510.10621017118580532No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCATGCT500.10412761880961305No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA500.10412761880961305No Hit
ATCTGCGCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT500.10412761880961305No Hit
ATCTGCGCACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT500.10412761880961305No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA500.10412761880961305No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCT500.10412761880961305No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA490.1020450664334208No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGATACCCACGCT490.1020450664334208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)