FastQCFastQC Report
Sun 21 May 2023
EGAF00007838019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47691
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14403.0194376297414607No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT14162.9691136692457696No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT12242.5665219852802417No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11692.4511962424776166No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8861.8577928749659265No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7571.5873015873015872No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7211.5118156465580508No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6981.4635885177496801No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6791.4237487156905915No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6741.4132645572539893No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6581.379715250256862No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6311.3231007946992095No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6011.2601958440795957No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5711.197290893459982No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5641.1826130716487389No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5471.146966932964291No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT5361.123901784403766No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG5271.1050302992178818No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5151.0798683189700362No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5131.0756746555953953No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4690.9834140613532951No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4540.9519615860434884No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA4430.9288964374829632No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4410.9247027741083224No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT4350.9121217839843996No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4320.9058312889224382No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4150.8701851502379904No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA4040.8471200016774653No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3930.8240548531169403No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3710.7779245559958902No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3650.7653435658719674No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3620.7590530708100061No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3530.740181585624122No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3510.7359879222494811No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT3430.7192132687509173No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3130.6563083181313035No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3100.6500178230693422No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA3070.6437273280073809No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT3030.6353400012580991No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2980.6248558428214968No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2910.6101780210102535No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2870.6017906942609716No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2720.5703382189511648No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2590.5430794070159988No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2560.5367889119540374No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2500.5242079218301148No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2420.507433268331551No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2400.5032396049569101No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2380.4990459415822692No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2370.49694910989494867No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2370.49694910989494867No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2340.49065861483298734No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2320.48646495145834645No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2230.4675934662724623No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2210.4633998028978214No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2170.45501247614853957No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2040.42775366421337363No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2010.4214631691514122No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1940.406785347340169No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1930.4046885156528486No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1930.4046885156528486No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1900.39839802059088714No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1900.39839802059088714No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1890.3963011889035667No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1880.39420435721624625No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1860.3900106938416053No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1770.3711392086557212No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1750.3669455452810803No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1680.3522677234698371No Hit
ATCTGCGCACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1680.3522677234698371No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1660.3480740600951962No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1610.33758990165859387No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1600.3354930699712734No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1570.32920257490931204No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1570.32920257490931204No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1560.32710574322199154No Hit
ATCTGCGCACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1540.32291207984735065No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1500.3145247530980688No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1500.3145247530980688No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1440.30194376297414605No Hit
ATCTGCGCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1390.29145960453754377No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT1360.2851691094755824No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1360.2851691094755824No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1340.2809754461009415No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT1330.278878614413621No Hit
ATCTGCGCACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1280.2683944559770187No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1190.2495229707911346No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1180.2474261391038141No Hit
ATCTGCGCACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1170.24532930741649367No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1130.23694198066721187No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1130.23694198066721187No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1110.23274831729257092No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT1100.23065148560525045No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCT1090.22855465391793No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1080.22645782223060956No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1080.22645782223060956No Hit
ATCTGCGCACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1080.22645782223060956No Hit
ATCTGCGCACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1070.22436099054328906No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1050.22016732716864817No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1050.22016732716864817No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1040.2180704954813277No Hit
ATCTGCGCACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1010.21178000041936632No Hit
ATCTGCGCACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT970.2033926736700845No Hit
ATCTGCGCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT950.19919901029544357No Hit
ATCTGCGCACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT950.19919901029544357No Hit
ATCTGCGCACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT950.19919901029544357No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.19500534692080265No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC900.1887148518588413No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT880.18452118848420038No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT870.1824243567968799No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT860.18032752510955946No Hit
ATCTGCGCACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT850.178230693422239No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT850.178230693422239No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT840.17613386173491855No Hit
ATCTGCGCACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT830.1740370300475981No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA830.1740370300475981No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC810.16984336667295716No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA780.16355287161099577No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCC770.16145603992367533No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.1572623765490344No Hit
ATCTGCGCACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT740.15516554486171394No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT730.1530687131743935No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT730.1530687131743935No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA720.15097188148707302No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC700.14677821811243214No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA700.14677821811243214No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG700.14677821811243214No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA700.14677821811243214No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCT700.14677821811243214No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT690.14468138642511166No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC680.1425845547377912No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA670.14048772305047075No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.14048772305047075No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA660.13839089136315028No Hit
ATCTGCGCACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC650.1362940596758298No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT650.1362940596758298No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCGCACC620.13000356461386844No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT620.13000356461386844No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.127906732926548No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT600.12580990123922753No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA590.12371306955190706No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC580.12161623786458661No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT580.12161623786458661No Hit
ATCTGCGCACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT570.11951940617726615No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA560.11742257448994568No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.11532574280262523No Hit
ATCTGCGCACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC550.11532574280262523No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCT540.11322891111530478No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG540.11322891111530478No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC540.11322891111530478No Hit
ATCTGCGCACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT530.11113207942798431No Hit
ATCTGCGCACCGGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCT530.11113207942798431No Hit
ATCTGCGCACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT530.11113207942798431No Hit
ATCTGCGCACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT520.10903524774066385No Hit
ATCTGCGCACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.10903524774066385No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA510.10693841605334341No Hit
ATCTGCGCACCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT510.10693841605334341No Hit
ATCTGCGCACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC500.10484158436602294No Hit
ATCTGCGCACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT500.10484158436602294No Hit
ATCTGCGCACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT500.10484158436602294No Hit
ATCTGCGCACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC500.10484158436602294No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA490.10274475267870248No Hit
ATCTGCGCACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT490.10274475267870248No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA480.10064792099138203No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)