FastQCFastQC Report
Sun 21 May 2023
EGAF00007838045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47449
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT20724.36679382073384No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA19644.139181015406015No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA18984.00008430103901No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT10352.1812893843916625No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9942.0948808194060993No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9832.071698033678265No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9191.9368163712617756No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8701.8335475984741512No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8531.7977196568947713No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7701.6227950009483867No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7261.5300638580370503No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7111.4984509684081857No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5931.2497629033277835No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT5861.23501022150098No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5521.16335433834222No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5091.0727307214061412No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5021.0579780395793377No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4881.0284726759257308No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4700.9905372083710932No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4550.9589243187422285No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4480.9441716369154249No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG4410.9294189550886215No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC4350.9167737992370756No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4170.878838331682438No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4100.8640856498556344No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4080.8598705979051192No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4030.849332968028831No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3920.8261501823009968No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3870.8156125524247086No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3660.7713545069442981No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3310.6975910978102805No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3280.6912685198845075No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT3250.6849459419587346No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT3180.6701932601319311No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3040.640687896478324No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3000.6322577925772935No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2930.61750511075049No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2870.6048599548989442No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2750.5795696431958524No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2670.5627094353937911No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2570.5416341756412149No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2560.5395266496659571No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2350.4952686041855466No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2330.4910535522350313No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2320.4889460262597737No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2290.4826234483340007No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2240.47208581845771247No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2220.4678707665071972No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2200.4636557145566819No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2190.46154818858142427No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2170.45733313663090897No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2070.4362578768783325No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2050.4320428249278172No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1960.4130750911504984No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1940.40886003919998315No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1930.40675251322472544No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1920.40464498724946785No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1900.4004299352989526No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1870.39410735737317965No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1690.35617188981854203No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1630.3435267339669962No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1560.3287740521401926No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1500.31612889628864677No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1490.3140213703133891No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1480.31191384433813146No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1480.31191384433813146No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1480.31191384433813146No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1470.3098063183628738No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1460.3076987923876162No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1440.30348374043710086No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1400.2950536365360703No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1390.29294611056081266No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1380.290838584585555No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1340.28240848068452445No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1340.28240848068452445No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1310.2760859027587515No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1290.2718708508082362No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1250.26344074690720565No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1220.2571181689814327No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1220.2571181689814327No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1200.2529031170309174No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.24868806508040212No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1170.2465805391051445No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1150.24236548715462916No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1130.23815043520411389No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1120.23604290922885623No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1120.23604290922885623No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1090.22972033130308334No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1090.22972033130308334No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1090.22972033130308334No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT1070.225505279352568No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT1010.21286012350102218No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT990.20864507155050685No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC960.20232249362473392No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA960.20232249362473392No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA950.2002149676494763No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC940.19810744167421865No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT940.19810744167421865No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.19599991569896097No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT890.18756981179793042No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA870.18335475984741512No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT850.17913970789689984No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT850.17913970789689984No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT840.17703218192164216No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT840.17703218192164216No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT830.17492465594638454No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA820.1728171299711269No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT810.17070960399586926No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA810.17070960399586926No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA800.16860207802061158No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT780.1643870260700963No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC780.1643870260700963No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA760.160171974119581No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA760.160171974119581No Hit
ATCAGATTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT740.15595692216906573No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA730.1538493961938081No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC700.14752681826803515No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT680.14331176631751985No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT680.14331176631751985No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA670.14120424034226223No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC670.14120424034226223No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA650.13698918839174692No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT640.13488166241648927No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT630.13277413644123165No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC620.130666610465974No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA620.130666610465974No Hit
ATCAGATTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT620.130666610465974No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA610.12855908449071635No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA600.1264515585154587No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA600.1264515585154587No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT600.1264515585154587No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT590.12434403254020106No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA590.12434403254020106No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT580.12223650656494342No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC570.12012898058968577No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT570.12012898058968577No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT560.11802145461442812No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.11591392863917048No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT550.11591392863917048No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA540.11380640266391283No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT540.11380640266391283No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC530.11169887668865519No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA530.11169887668865519No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA530.11169887668865519No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG520.10959135071339754No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA520.10959135071339754No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC520.10959135071339754No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCCGGCT520.10959135071339754No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT520.10959135071339754No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT510.1074838247381399No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.1074838247381399No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTA510.1074838247381399No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT500.10537629876288225No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC490.1032687727876246No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC490.1032687727876246No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA490.1032687727876246No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA480.10116124681236696No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG480.10116124681236696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)