FastQCFastQC Report
Sun 21 May 2023
EGAF00007838049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39559
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT19034.810536161176976No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA15854.006673576177356No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA14463.65529967896054No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA9752.464673020046007No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA9732.459617280517708No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9532.4090598852347127No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8422.1284663414140903No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7831.979322025329255No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6621.6734497838671352No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6541.653226825753937No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5591.4130791981597108No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT5561.4054955888672616No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC5561.4054955888672616No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT5421.3701054121691651No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5351.3524103238201168No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4831.2209610960843296No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4801.2133774867918805No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4571.1552364822164363No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4531.1451250031598372No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4261.076872519527794No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4111.0389544730655476No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3750.9479511615561567No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3660.9252003336788088No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3350.8468363709901666No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3320.8392527616977172No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3060.7735281478298238No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2980.7533051897166257No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2960.7482494501883263No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2890.730554361839278No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA2760.6976920549053313No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2760.6976920549053313No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT2700.6825248363204328No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2670.6749412270279835No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2570.649662529386486No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA2550.6446067898581865No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2440.6168002224525393No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2390.6041608736317905No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2370.599105134103491No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2210.5586592178770949No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2200.5561313481129452No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2190.5536034783487954No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC2160.5460198690563461No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2090.5283247807072979No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1970.49799034353750093No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1960.4954624737733512No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1950.4929346040092014No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1940.49040673424505166No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1920.4853509947167522No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1910.48282312495260243No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1910.48282312495260243No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1880.47523951566015316No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT1860.47018377613185375No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT1820.46007229707525465No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1760.4449050784903562No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1750.4423772087262064No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1720.4347935994337571No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1660.4196263808488586No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1660.4196263808488586No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1650.41709851108470886No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1600.40445916226396017No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1600.40445916226396017No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1600.40445916226396017No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1530.3867640739149119No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1520.38423620415076215No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1490.3766525948583129No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1470.3715968553300134No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1410.35642963674511485No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1400.35390176698096515No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1370.3463181576885159No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1350.3412624181602164No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1310.33115093910361737No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1250.31598372051871887No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1210.3058722414621199No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1200.3033443716979701No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1180.2982886321696706No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1160.2932328926413711No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1160.2932328926413711No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1150.2907050228772214No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1120.2831214135847721No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.27300993452817307No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1080.27300993452817307No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC1070.27048206476402337No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1060.2679541949998736No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1040.26289845547157414No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA980.2477312368866756No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT980.2477312368866756No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG980.2477312368866756No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG960.2426754973583761No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT960.2426754973583761No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC950.24014762759422634No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT940.23761975783007658No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT900.22750827877347757No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT890.22498040900932786No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT880.2224525392451781No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC860.21739679971687856No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA840.2123410601885791No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA800.20222958113198009No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA770.19464597183953083No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT750.18959023231123132No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA740.18706236254708158No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC740.18706236254708158No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.18453449278293182No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.18453449278293182No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC720.1820066230187821No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT720.1820066230187821No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG710.1794787532546323No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT700.17695088349048257No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT700.17695088349048257No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA680.17189514396218308No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA650.1643115346697338No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT650.1643115346697338No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT640.16178366490558407No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT620.15672792537728455No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC610.15420005561313482No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.15420005561313482No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC600.15167218584898506No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT590.1491443160848353No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA580.14661644632068555No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG570.1440885765565358No Hit
ATCAGATTGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT560.14156070679238605No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.14156070679238605No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC560.14156070679238605No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA560.14156070679238605No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT540.13650496726408654No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT540.13650496726408654No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT530.1339770974999368No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC530.1339770974999368No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.1339770974999368No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT530.1339770974999368No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA520.13144922773578707No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG520.13144922773578707No Hit
ATCAGATTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT520.13144922773578707No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC510.12892135797163728No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT500.12639348820748755No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA490.1238656184433378No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC490.1238656184433378No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC480.12133774867918805No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT480.12133774867918805No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA470.11880987891503829No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG470.11880987891503829No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT470.11880987891503829No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.11880987891503829No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.11880987891503829No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT450.11375413938673878No Hit
ATCAGATTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT450.11375413938673878No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT440.11122626962258905No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT400.10111479056599004No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA400.10111479056599004No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC400.10111479056599004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)