FastQCFastQC Report
Sun 21 May 2023
EGAF00007838062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41107
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT12543.0505753278030503No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG12523.045709976403046No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9402.286715158002287No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8472.0604763179020607No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT8131.9777653441019778No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7531.8318048021018318No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT7491.8220740993018223No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6541.5909699078015909No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA6341.5423163938015423No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6241.517989636801518No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5971.4523073929014523No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5961.44987471720145No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5941.4450093658014451No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5551.3501350135013501No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5511.3404043107013404No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5091.2382319313012382No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4821.1725496874011725No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4791.1652516603011653No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA4721.1482229304011482No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA4391.067944632301068No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4231.029021821101029No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4221.0265891454010265No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC4131.0046950641010048No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4050.9852336585009853No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3970.9657722529009658No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3940.9584742258009584No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3750.9122533875009122No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3590.8733305763008733No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3570.8684652249008684No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3430.8344077651008345No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3400.8271097380008271No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3370.8198117109008198No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3200.7784562240007784No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3150.7662928455007663No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3070.7468314399007467No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2980.724937358600725No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2980.724937358600725No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2980.724937358600725No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2870.6981779259006982No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2700.6568224390006568No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2640.6422263848006422No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2640.6422263848006422No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT2620.6373610334006374No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2440.5935728708005935No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2420.5887075194005887No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2390.5814094923005814No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2370.5765441409005766No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2340.5692461138005692No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2230.5424866811005424No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2140.5205925998005205No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2090.5084292213005084No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC2050.49869851850049873No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2040.4962658428004963No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2020.4914004914004914No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1910.4646410587004646No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1890.4597757073004598No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1860.4524776802004525No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1860.4524776802004525No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1820.4427469774004427No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT1760.42815092320042814No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1700.41355486900041355No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1650.4013914905004014No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1650.4013914905004014No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1610.39166078770039164No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1590.3867954363003868No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1530.3721993821003722No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1500.3649013550003649No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1470.3576033279003576No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1450.3527379765003527No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC1440.3503053008003503No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1420.34543994940034545No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1380.3357092466003357No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1370.33327657090033325No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1360.33084389520033086No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1310.3186805167003187No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1250.3040844625003041No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1230.29921911110029925No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1190.2894884083002895No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1160.28219038120028217No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1130.2748923541002749No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1130.2748923541002749No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1120.27245967840027246No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1100.2675943270002676No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1090.26516165130026514No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1090.26516165130026514No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1080.26272897560026276No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1070.26029629990026026No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1000.24326757000024327No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA990.24083489430024083No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC980.2384022186002384No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT920.22380616440022383No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT920.22380616440022383No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC910.22137348870022136No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA900.21894081300021895No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT860.2092101102002092No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG860.2092101102002092No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT850.20677743450020677No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT840.20434475880020436No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA830.2019120831002019No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT830.2019120831002019No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.19704673170019704No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT810.19704673170019704No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA790.1921813803001922No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA790.1921813803001922No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT790.1921813803001922No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT780.18974870460018975No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC770.18731602890018734No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA770.18731602890018734No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT770.18731602890018734No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA750.18245067750018246No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC750.18245067750018246No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC720.17515265040017516No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT710.17271997470017272No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG710.17271997470017272No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.17271997470017272No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA700.17028729900017028No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA700.17028729900017028No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG690.16785462330016784No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.162989271900163No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA660.16055659620016055No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC640.1556912448001557No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT640.1556912448001557No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC640.1556912448001557No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT640.1556912448001557No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC630.15325856910015326No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG620.15082589340015082No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT620.15082589340015082No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC620.15082589340015082No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.14839321770014838No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT610.14839321770014838No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC600.14596054200014597No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT590.14352786630014353No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT580.14109519060014108No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT570.13866251490013864No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA570.13866251490013864No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT550.1337971635001338No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC550.1337971635001338No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT550.1337971635001338No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA550.1337971635001338No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA540.13136448780013138No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG530.12893181210012894No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA510.12406646070012407No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC480.11676843360011677No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC480.11676843360011677No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCT470.11433575790011433No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT460.11190308220011191No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA460.11190308220011191No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT450.10947040650010947No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC450.10947040650010947No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG450.10947040650010947No Hit
ATCAGATTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT440.10703773080010703No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA440.10703773080010703No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA440.10703773080010703No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC430.1046050551001046No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT430.1046050551001046No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCGCACC430.1046050551001046No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG420.10217237940010218No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA420.10217237940010218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)