FastQCFastQC Report
Sun 21 May 2023
EGAF00007838063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41107
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT12693.087065463303087No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG11652.834067190502834No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9882.4034835916024035No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8742.1261585618021264No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7581.843968180601844No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7511.8269394507018268No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT7471.8172087479018173No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7381.7953146666017952No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7031.7101710171017102No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6461.5715085022015716No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6311.5350183667015351No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5861.4255479602014256No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA5591.3598657163013599No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5391.3112122023013113No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5091.2382319313012382No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4991.213905174301214No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4931.1993091201011992No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4711.1457902547011458No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4581.1141654706011141No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC4381.0655119566010653No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4351.0582139295010582No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA4271.0387525239010387No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4261.0363198482010363No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4211.0241564697010241No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4060.9876663342009876No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4060.9876663342009876No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3860.9390128202009389No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3560.8660325492008659No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3280.7979176296007979No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3210.7808888997007809No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3190.776023548300776No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3140.7638601698007639No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3060.7443987642007444No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2950.7176393315007177No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2870.6981779259006982No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2790.6787165203006787No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2760.6714184932006714No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2750.668985817500669No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2750.668985817500669No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2700.6568224390006568No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2570.6251976549006252No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2500.6081689250006082No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2490.6057362493006058No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2470.6008708979006009No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2400.5838421680005839No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2380.578976816600579No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2380.578976816600579No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2280.5546500596005546No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2270.5522173839005522No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2170.5278906269005279No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2060.5011311942005011No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2030.4938331671004938No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT2010.488967815700489No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1940.47193908580047195No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1870.45491035590045487No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1770.4305835989004306No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1770.4305835989004306No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1710.415987544700416No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1690.41112219330041117No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1660.4038241662004038No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1660.4038241662004038No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1650.4013914905004014No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1650.4013914905004014No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1600.3892281120003892No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1590.3867954363003868No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1540.3746320578003747No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1530.3721993821003722No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1510.36733403070036735No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1390.3381419223003381No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1380.3357092466003357No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1340.325978543800326No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1270.30894981390030896No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1260.3065171382003065No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1230.29921911110029925No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1210.29435375970029437No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1200.29192108400029193No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1140.2773250298002773No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1140.2773250298002773No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1100.2675943270002676No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.26272897560026276No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1060.2578636242002579No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1060.2578636242002579No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1050.25543094850025544No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1040.252998272800253No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT1030.25056559710025056No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1010.2457002457002457No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1010.2457002457002457No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1010.2457002457002457No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1010.2457002457002457No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA980.2384022186002384No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT970.23596954290023597No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC960.23353686720023353No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT930.22623884010022624No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA920.22380616440022383No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT920.22380616440022383No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA900.21894081300021895No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA900.21894081300021895No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA890.21650813730021648No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT870.21164278590021166No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA860.2092101102002092No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC850.20677743450020677No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC850.20677743450020677No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT850.20677743450020677No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA840.20434475880020436No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT830.2019120831002019No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA780.18974870460018975No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT770.18731602890018734No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA760.1848833532001849No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA750.18245067750018246No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA740.18001800180018002No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC730.1775853261001776No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT720.17515265040017516No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG720.17515265040017516No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.17515265040017516No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC710.17271997470017272No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT700.17028729900017028No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA690.16785462330016784No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT680.16542194760016543No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC650.1581239205001581No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT640.1556912448001557No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG630.15325856910015326No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG620.15082589340015082No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC620.15082589340015082No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA620.15082589340015082No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA610.14839321770014838No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC580.14109519060014108No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT580.14109519060014108No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA570.13866251490013864No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT570.13866251490013864No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA570.13866251490013864No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC560.13622983920013623No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT560.13622983920013623No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA550.1337971635001338No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC540.13136448780013138No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG540.13136448780013138No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT540.13136448780013138No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG540.13136448780013138No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA540.13136448780013138No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC530.12893181210012894No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA520.1264991364001265No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT520.1264991364001265No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG510.12406646070012407No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACT510.12406646070012407No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT500.12163378500012163No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.1192011093001192No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC480.11676843360011677No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCC480.11676843360011677No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG470.11433575790011433No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT470.11433575790011433No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT470.11433575790011433No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA460.11190308220011191No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC460.11190308220011191No Hit
ATCAGATTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT450.10947040650010947No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA440.10703773080010703No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT440.10703773080010703No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT430.1046050551001046No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCT430.1046050551001046No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT430.1046050551001046No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA430.1046050551001046No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCGCACC430.1046050551001046No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT420.10217237940010218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)