FastQCFastQC Report
Sun 21 May 2023
EGAF00007838066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39030
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA540613.85088393543428No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT31648.106584678452473No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA30217.740199846272098No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA28687.348193697156034No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA16174.142966948501153No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT14913.8201383551114527No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT13443.4435049961568023No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT10192.610812195746861No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT10072.580066615424033No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7411.8985395849346658No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6551.6781962592877273No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT5791.4834742505764795No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4471.1452728670253651No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4371.1196515500896747No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4031.032539072508327No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3510.8993082244427363No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3270.8378170637970792No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3270.8378170637970792No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3170.8121957468613886No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3080.7891365616192673No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2980.7635152446835767No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2640.676402767102229No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2140.5482961824237765No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2110.5406097873430694No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2050.5252369971816552No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA1970.5047399436331027No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1960.5021778119395337No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1940.4970535485523956No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1930.4944914168588266No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1830.46887009992313605No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA1820.466307968229567No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1730.4432487829874455No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1720.4406866512938765No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1710.43812451960030746No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1640.42018959774532416No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT1570.4022546758903407No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT1520.3894440174224955No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1440.3689469638739431No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1260.3228285933897002No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1180.3023315398411478No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA1160.29720727645400974No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1140.2920830130668716No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1120.2869587496797335No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1120.2869587496797335No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1110.2843966179861645No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1110.2843966179861645No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.26646169613118115No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG990.2536510376633359No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG990.2536510376633359No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA950.24340251088905968No Hit
ATCAGATTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT950.24340251088905968No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG930.23827824750192161No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.23571611580835256No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA910.2331539841147835No Hit
ATCAGATTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT860.22034332564693826No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA840.21521906225980014No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA830.2126569305662311No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT830.2126569305662311No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT820.21009479887266208No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA780.19984627209838585No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT740.18959774532410967No Hit
ATCAGATTGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT710.18191135024340252No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG700.17934921854983346No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC680.17422495516269537No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.16910069177555725No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG660.16910069177555725No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA650.1665385600819882No Hit
ATCAGATTGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT640.16397642838841917No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC630.1614142966948501No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT620.15885216500128105No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA610.15629003330771202No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA590.1511657699205739No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA560.14347937483986675No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT550.14091724314629772No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.13579297975915963No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA520.13323084806559057No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC520.13323084806559057No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.13066871637202154No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT500.12810658467845248No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA490.12554445298488343No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA490.12554445298488343No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA490.12554445298488343No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT480.12298232129131438No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.11785805790417628No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT460.11785805790417628No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT450.11529592621060722No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA450.11529592621060722No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA440.11273379451703817No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA440.11273379451703817No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC440.11273379451703817No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT440.11273379451703817No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA430.11017166282346913No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT430.11017166282346913No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA410.10504739943633104No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA410.10504739943633104No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG410.10504739943633104No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA400.10248526774276198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)