FastQCFastQC Report
Sun 21 May 2023
EGAF00007838071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60931
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA50698.319246360637441No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA34745.701531240255371No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT33385.47832794472436No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA25644.2080386010405215No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17282.8359948138057804No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT16842.7637819828986885No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG16732.7457287751719157No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT12932.1220725082470335No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT12762.094172096305657No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG11251.8463507902381382No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT11121.8250151811064974No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9951.632994698921731No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT9661.585399878551148No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT8791.4426154174393988No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT7901.2965485549227815No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7211.1833057064548422No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6941.138993287489127No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6851.124222481167222No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6471.0618568544747338No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT6040.991285224270076No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5230.85834796737293No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT5010.8222415519193843No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4820.7910587385731402No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4640.7615171259293299No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4480.7352579146903875No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4460.7319755132855197No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4110.6745334887003331No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT4090.6712510872954653No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4060.6663274851881636No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4000.6564802809735603No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3750.6154502634127128No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3740.6138090627102789No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT3680.6039618584956754No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3670.6023206577932415No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3490.5727790451494313No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3480.5711378444469974No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3470.5694966437445635No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3460.5678554430421296No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3140.5153370205642448No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3120.512054619159377No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3080.5054898163496414No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT3020.495642612135038No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2990.4907190100277363No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2970.4874366086228685No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2930.4808718058131329No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2910.4775894044082651No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2880.4726658023009634No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2580.4234297812279464No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2410.3955293692865701No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2360.3873233657744005No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2290.37583496085736323No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT2270.37255255945249544No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2240.36762895734519374No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA2180.35778175313059035No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2120.3479345489159869No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2070.3397285454038174No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2040.3348049432965157No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2010.32988134118921403No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1980.32495773908191233No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1980.32495773908191233No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1970.32331653837947844No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1920.3151105348673089No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1860.30526333065270556No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1800.2954161264381021No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1780.2921337250332343No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1750.2872101229259326No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1740.2855689222234987No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1730.2839277215210648No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1730.2839277215210648No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1680.2757217180088953No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1680.2757217180088953No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1670.27408051730646144No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1630.2675157144967258No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1550.2543861088772546No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1510.247821306067519No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1470.2412565032577834No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1460.2396153025553495No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1380.22648569693587828No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1370.2248444962334444No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1370.2248444962334444No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1360.2232032955310105No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT1350.2215620948285766No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1330.2182796934237088No Hit
ATCAGATTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1230.20186768639936978No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT1220.20022648569693588No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1220.20022648569693588No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.19037928148233246No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1130.18545567937503077No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1110.18217327797016297No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT1090.17889087656529518No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1080.17724967586286128No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1080.17724967586286128No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1070.17560847516042735No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA970.15919646813608837No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT940.15427286602878668No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA920.15099046462391885No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA920.15099046462391885No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT910.14934926392148495No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA870.14278446111174936No Hit
ATCAGATTGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT840.13786085900444764No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT830.13621965830201377No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA830.13621965830201377No Hit
ATCAGATTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT810.13293725689714594No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA810.13293725689714594No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT810.13293725689714594No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT800.13129605619471205No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.13129605619471205No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA790.12965485549227815No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA790.12965485549227815No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT770.12637245408741035No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT740.12144885198010864No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.11980765127767475No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA710.11652524987280696No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA700.11488404917037304No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT700.11488404917037304No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA700.11488404917037304No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA680.11160164776550525No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT680.11160164776550525No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT680.11160164776550525No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT670.10996044706307133No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC660.10831924636063744No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC650.10667804565820355No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC650.10667804565820355No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCT650.10667804565820355No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC650.10667804565820355No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCT640.10503684495576965No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT640.10503684495576965No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA630.10339564425333574No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA620.10175444355090184No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA610.10011324284846794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)