FastQCFastQC Report
Sun 21 May 2023
EGAF00007838072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48625
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA16073.304884318766067No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT15623.2123393316195377No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11932.453470437017995No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG11662.397943444730077No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11462.3568123393316194No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA9681.990745501285347No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8891.8282776349614398No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8031.651413881748072No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7961.6370179948586119No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7411.5239074550128535No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7361.513624678663239No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7131.4663239074550127No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6821.4025706940874036No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6421.3203084832904883No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6291.293573264781491No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6011.2359897172236505No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5701.1722365038560412No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT5511.1331619537275064No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5351.1002570694087404No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5261.0817480719794346No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5161.0611825192802058No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4730.9727506426735218No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC4630.9521850899742932No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT4600.9460154241645244No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4540.9336760925449871No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4260.8760925449871466No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4220.867866323907455No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4180.8596401028277636No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3730.767095115681234No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3710.7629820051413881No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3660.7526992287917738No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3570.7341902313624679No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3560.732133676092545No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3530.7259640102827764No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3440.7074550128534705No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3420.7033419023136247No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3140.645758354755784No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3020.6210796915167095No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2990.6149100257069409No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2630.5408740359897173No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2590.5326478149100258No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2560.526478149100257No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2420.49768637532133675No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2390.4915167095115682No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC2350.48329048843187666No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2350.48329048843187666No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2330.47917737789203085No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2260.46478149100257066No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2260.46478149100257066No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2240.46066838046272496No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2190.4503856041131105No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2100.4318766066838046No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2060.4236503856041131No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2060.4236503856041131No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2050.4215938303341903No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2040.4195372750642673No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2010.4133676092544987No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1960.4030848329048843No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1950.40102827763496146No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1940.3989717223650386No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1880.38663239074550126No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1880.38663239074550126No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1850.38046272493573263No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1820.374293059125964No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1720.3537275064267352No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1710.35167095115681235No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1640.3372750642673522No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1580.32493573264781495No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1490.306426735218509No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1490.306426735218509No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1470.30231362467866324No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1470.30231362467866324No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1460.30025706940874036No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1450.2982005141388175No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1450.2982005141388175No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1440.2961439588688946No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1440.2961439588688946No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1400.2879177377892031No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1350.2776349614395887No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1350.2776349614395887No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1340.2755784061696658No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1340.2755784061696658No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1290.26529562982005145No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1280.2632390745501285No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1280.2632390745501285No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1270.26118251928020564No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT1270.26118251928020564No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1270.26118251928020564No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1250.2570694087403599No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1250.2570694087403599No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1220.25089974293059125No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC1200.2467866323907455No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1190.24473007712082262No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1180.24267352185089974No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1170.24061696658097687No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1120.23033419023136248No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1110.22827763496143957No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1100.22622107969151672No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1080.22210796915167097No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1070.22005141388174806No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1030.21182519280205656No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1030.21182519280205656No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1000.2056555269922879No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.2056555269922879No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA990.20359897172236505No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA980.20154241645244214No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT940.19331619537275063No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA910.187146529562982No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT910.187146529562982No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC900.18508997429305912No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC890.18303341902313625No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT880.18097686375321337No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA870.1789203084832905No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA870.1789203084832905No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT870.1789203084832905No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA860.1768637532133676No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT860.1768637532133676No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT860.1768637532133676No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC850.17480719794344474No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA820.1686375321336761No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA820.1686375321336761No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT800.16452442159383035No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA790.16246786632390747No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC780.1604113110539846No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC780.1604113110539846No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA770.15835475578406172No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC730.15012853470437018No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA710.14601542416452443No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT700.14395886889460155No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA670.1377892030848329No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT670.1377892030848329No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC660.13573264781491No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA660.13573264781491No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA650.13367609254498714No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT650.13367609254498714No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT630.12956298200514138No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA630.12956298200514138No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC620.1275064267352185No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA620.1275064267352185No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA620.1275064267352185No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT610.12544987146529563No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT610.12544987146529563No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA610.12544987146529563No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.12339331619537275No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT600.12339331619537275No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG590.12133676092544987No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC580.119280205655527No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT570.11722365038560412No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT570.11722365038560412No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG560.11516709511568124No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT560.11516709511568124No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT550.11311053984575836No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT550.11311053984575836No Hit
ATCAGATTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT540.11105398457583548No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC540.11105398457583548No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACT540.11105398457583548No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.11105398457583548No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA530.10899742930591258No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC530.10899742930591258No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT520.1069408740359897No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC510.10488431876606683No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC510.10488431876606683No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA500.10282776349614395No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.10077120822622107No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG490.10077120822622107No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG490.10077120822622107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)