FastQCFastQC Report
Sun 21 May 2023
EGAF00007838082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44932
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA25125.590670346301077No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT24585.4704887385382355No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA23175.156681207157483No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG11762.6172883468352177No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11252.5037834950592006No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG11082.465948544467195No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG9432.0987269651918457No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9202.0475385026261907No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9102.0252826493367753No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7851.7470844832190868No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7531.6758657526929583No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT7281.6202261194694205No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7091.5779399982195317No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6941.5445562182854091No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6291.3998931719042107No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5981.330900026707024No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5871.3064185880886674No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5521.2285231015757145No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5421.2062672482862993No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4701.0460251046025104No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4621.0282204219709783No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3560.7923083771031781No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3520.7834060357874121No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3500.778954865129529No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3440.7656013531558801No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3430.7633757678269385No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3340.7433454998664649No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3200.7121873052612837No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2830.6298406480904478No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2760.6142615507878573No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2670.5942312828273836No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2630.5853289415116175No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2590.5764266001958516No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2590.5764266001958516No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA2480.551945161577495No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2470.5497195762485534No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2390.5319148936170213No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2390.5319148936170213No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA2230.49630552835395714No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2200.48962877236713254No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2010.44734265111724386No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1910.42508679782782877No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1890.4206356271699457No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1890.4206356271699457No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA1890.4206356271699457No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1840.40950770052523816No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1820.40505652986735513No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1810.40283094453841356No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA1810.40283094453841356No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1810.40283094453841356No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1810.40283094453841356No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1790.3983797738805306No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1740.387251847235823No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1660.36944716460429095No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1610.35831923795958337No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1580.3516424819727588No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1580.3516424819727588No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1530.34051455532805125No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1520.3382889699991098No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1490.33161221401228524No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1490.33161221401228524No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT1490.33161221401228524No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1480.32938662868334373No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1480.32938662868334373No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.32048428736757767No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1400.31158194605181166No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1350.30045401940710403No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1330.29600284874922106No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1320.29377726342027954No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1290.287100507433455No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1290.287100507433455No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1270.28264933677557197No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1210.2692958248019229No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1190.26484465414403985No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1170.2603934834861569No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC980.21810736223626814No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT970.21588177690732663No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA950.2114306062494436No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA950.2114306062494436No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA940.20920502092050208No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC940.20920502092050208No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA940.20920502092050208No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA910.20252826493367757No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT870.1936259236179115No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC860.19140033828897002No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT800.17804682631532093No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA800.17804682631532093No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT790.1758212409863794No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA770.1713700703284964No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT770.1713700703284964No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC750.16691889967061338No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT740.16469331434167186No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT730.16246772901273035No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA730.16246772901273035No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT720.16024214368378883No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA710.15801655835484735No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.15801655835484735No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT700.15579097302590583No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT700.15579097302590583No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT690.15356538769696432No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA680.1513398023680228No Hit
ATCAGATTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT670.1491142170390813No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT670.1491142170390813No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT660.14688863171013977No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT650.14466304638119826No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA640.14243746105225674No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA640.14243746105225674No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA640.14243746105225674No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA620.13798629039437374No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT620.13798629039437374No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG600.1335351197364907No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC600.1335351197364907No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT590.1313095344075492No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA590.1313095344075492No Hit
ATCAGATTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT590.1313095344075492No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC570.12685836374966616No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA570.12685836374966616No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT570.12685836374966616No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTG560.12463277842072465No Hit
ATCAGATTGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT550.12240719309178313No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT550.12240719309178313No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA550.12240719309178313No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT550.12240719309178313No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA540.12018160776284163No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT540.12018160776284163No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG540.12018160776284163No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA540.12018160776284163No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC540.12018160776284163No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC530.11795602243390012No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT530.11795602243390012No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACACT530.11795602243390012No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCTAGCT530.11795602243390012No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA520.1157304371049586No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA520.1157304371049586No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT500.11127926644707559No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA500.11127926644707559No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT500.11127926644707559No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA490.10905368111813407No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA490.10905368111813407No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT470.10460251046025104No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG470.10460251046025104No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC470.10460251046025104No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG470.10460251046025104No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCGCACC460.10237692513130954No Hit
ATCAGATTGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT460.10237692513130954No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA450.10015133980236803No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA450.10015133980236803No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTG450.10015133980236803No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA450.10015133980236803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)