FastQCFastQC Report
Sun 21 May 2023
EGAF00007838083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44932
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT26175.824356805839936No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA22735.058755452684056No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA19764.397756609988427No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT12972.8865841716371405No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG11042.457046203151429No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG11002.448143861835663No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8781.9540639188106472No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8481.8872963589424019No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8021.7849194338110923No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT8001.7804682631532094No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT7641.700347191311315No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6951.5467818036143506No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6881.5312027063117601No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6501.4466304638119825No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6011.3375767826938485No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5961.326448856049141No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5211.1595299563785275No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5041.121695005786522No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5021.117243835128639No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4641.0326715926288614No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4320.9614528621027331No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4100.9124899848660198No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3840.8546247663135405No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3450.7678269384848214No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3430.7633757678269385No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3260.7255408172349328No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3150.7010593786165762No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3140.6988337932876346No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2850.6342918187483307No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2800.6231638921036233No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2470.5497195762485534No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2470.5497195762485534No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2460.5474939909196118No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2410.5363660642749043No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA2390.5319148936170213No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2310.5141102109854891No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2300.5118846256565477No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2300.5118846256565477No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2290.5096590403276061No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2280.5074334549986647No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2270.5052078696697232No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA2190.4874031870381911No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2190.4874031870381911No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2110.46959850440665896No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2000.44511706578830235No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1940.43176355381465326No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1900.4228612124988872No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1880.41841004184100417No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1870.4161844565120627No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1750.3894774325647645No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1740.387251847235823No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1700.378349505920057No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1680.373898335262174No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1670.37167274993323246No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1670.37167274993323246No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1650.36722157927534943No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1610.35831923795958337No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1570.3494168966438173No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1530.34051455532805125No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1520.3382889699991098No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1520.3382889699991098No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1510.3360633846701683No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1500.33383779934122676No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1380.30713077539392863No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1380.30713077539392863No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1350.30045401940710403No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1330.29600284874922106No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1320.29377726342027954No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1280.2848749221045135No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1250.27819816611768894No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1230.2737469954598059No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1170.2603934834861569No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1160.25816789815721536No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1100.24481438618356627No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1100.24481438618356627No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1020.22700970355203418No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1020.22700970355203418No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1010.22478411822309266No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT980.21810736223626814No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT970.21588177690732663No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT940.20920502092050208No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA920.20475385026261908No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA920.20475385026261908No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA910.20252826493367757No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC900.20030267960473605No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC890.19807709427579454No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT860.19140033828897002No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC860.19140033828897002No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT860.19140033828897002No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA860.19140033828897002No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA850.1891747529600285No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT850.1891747529600285No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA850.1891747529600285No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA840.186949167631087No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA800.17804682631532093No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT790.1758212409863794No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA780.17359565565743793No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.1691444849995549No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT750.16691889967061338No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCGCACC730.16246772901273035No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT720.16024214368378883No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC720.16024214368378883No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA710.15801655835484735No Hit
ATCAGATTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT710.15801655835484735No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC700.15579097302590583No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT700.15579097302590583No Hit
ATCAGATTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT690.15356538769696432No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA690.15356538769696432No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT680.1513398023680228No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT680.1513398023680228No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACACT670.1491142170390813No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA660.14688863171013977No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA650.14466304638119826No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT650.14466304638119826No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC650.14466304638119826No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA650.14466304638119826No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC640.14243746105225674No Hit
ATCAGATTGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT620.13798629039437374No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG610.13576070506543222No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA600.1335351197364907No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG590.1313095344075492No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA580.12908394907860768No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.12685836374966616No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA570.12685836374966616No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA570.12685836374966616No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT560.12463277842072465No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA560.12463277842072465No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC550.12240719309178313No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.12240719309178313No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG550.12240719309178313No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC540.12018160776284163No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTG540.12018160776284163No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG530.11795602243390012No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC530.11795602243390012No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC520.1157304371049586No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG520.1157304371049586No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.11127926644707559No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG490.10905368111813407No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT490.10905368111813407No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT490.10905368111813407No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT490.10905368111813407No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA480.10682809578919256No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA480.10682809578919256No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT470.10460251046025104No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA470.10460251046025104No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT460.10237692513130954No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT460.10237692513130954No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA450.10015133980236803No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA450.10015133980236803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)