FastQCFastQC Report
Sun 21 May 2023
EGAF00007838091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43720
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT16723.8243366880146388No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG12912.95288197621226No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12632.8888380603842636No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA11102.5388838060384264No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT11002.516010978956999No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8421.9258920402561757No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8301.898444647758463No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8011.8321134492223237No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT7771.7772186642268983No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6941.5873741994510522No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6931.5850869167429096No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6421.4684354986276305No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG6391.4615736505032022No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5961.363220494053065No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5881.344922232387923No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT5451.2465690759377859No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5441.244281793229643No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5421.2397072278133576No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5271.2053979871912168No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5141.1756633119853614No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5121.171088746569076No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4531.0361390667886552No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4471.0224153705397987No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG4451.0178408051235133No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4441.0155535224153704No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4030.9217749313815188No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3980.9103385178408051No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3840.878316559926807No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3830.8760292772186642No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3710.8485818847209515No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3590.8211344922232389No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3570.8165599268069532No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3520.8051235132662397No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3490.7982616651418115No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3130.7159194876486734No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3070.702195791399817No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2940.6724611161939615No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2900.6633119853613907No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG2770.6335773101555352No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2760.6312900274473925No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT2630.601555352241537No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2620.5992680695333943No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2600.5946935041171089No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2510.5741079597438243No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2490.5695333943275389No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2330.5329368709972552No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2330.5329368709972552No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2330.5329368709972552No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2220.5077767612076852No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2180.4986276303751144No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2030.4643183897529735No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG2020.4620311070448307No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1950.44602012808783165No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1860.4254345837145472No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1850.42314730100640435No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1820.4162854528819762No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1810.4139981701738335No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1800.41171088746569073No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1780.4071363220494053No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1760.4025617566331198No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1720.393412625800549No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1620.3705397987191217No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1530.34995425434583716No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1510.3453796889295517No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1500.34309240622140896No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1500.34309240622140896No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1490.34080512351326625No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1480.33851784080512354No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1470.33623055809698077No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1360.31107044830741076No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1360.31107044830741076No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1330.3042086001829826No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1310.29963403476669714No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1310.29963403476669714No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1240.2836230558096981No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1240.2836230558096981No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1220.2790484903934126No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1200.2744739249771272No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1180.26989935956084177No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1150.2630375114364135No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1150.2630375114364135No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1130.2584629460201281No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1130.2584629460201281No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1080.24702653247941445No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1030.23559011893870083No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1020.23330283623055809No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1010.23101555352241535No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1010.23101555352241535No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT990.22644098810612992No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT990.22644098810612992No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT990.22644098810612992No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA970.22186642268984444No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT950.217291857273559No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT920.21043000914913085No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT900.20585544373284537No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA890.20356816102470265No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC860.1967063129002745No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA840.192131747483989No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT830.1898444647758463No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC830.1898444647758463No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT830.1898444647758463No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT820.18755718206770355No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT810.18526989935956084No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.18526989935956084No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT800.18298261665141813No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA790.1806953339432754No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA790.1806953339432754No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA770.17612076852698993No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC760.17383348581884722No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT750.17154620311070448No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA740.16925892040256177No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA740.16925892040256177No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT730.16697163769441903No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT720.1646843549862763No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA700.16010978956999083No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT700.16010978956999083No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA700.16010978956999083No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG690.15782250686184812No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT690.15782250686184812No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT680.15553522415370538No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA670.15324794144556267No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA670.15324794144556267No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC650.14867337602927722No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA650.14867337602927722No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC640.1463860933211345No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA630.14409881061299176No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA610.1395242451967063No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA600.1372369624885636No Hit
ATCAGATTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT590.13494967978042088No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA580.13266239707227814No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA570.1303751143641354No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA570.1303751143641354No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT570.1303751143641354No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.1303751143641354No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT560.1280878316559927No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA550.12580054894784995No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA550.12580054894784995No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA550.12580054894784995No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT540.12351326623970722No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT530.1212259835315645No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA520.11893870082342177No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA510.11665141811527904No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC510.11665141811527904No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA510.11665141811527904No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA500.11436413540713633No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA500.11436413540713633No Hit
ATCAGATTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT490.11207685269899359No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT490.11207685269899359No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT480.10978956999085088No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT480.10978956999085088No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCT470.10750228728270814No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA470.10750228728270814No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC470.10750228728270814No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT470.10750228728270814No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC460.10521500457456542No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA460.10521500457456542No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA460.10521500457456542No Hit
ATCAGATTGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT450.10292772186642268No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC450.10292772186642268No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT450.10292772186642268No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC450.10292772186642268No Hit
ATCAGATTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT450.10292772186642268No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT440.10064043915827996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)