FastQCFastQC Report
Sun 21 May 2023
EGAF00007838125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46126
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT16213.514286953128387No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14853.2194423969128043No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14693.184754802063912No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10022.172310627411872No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9982.163638728699649No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9512.061743918831028No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9262.007544551879634No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8761.899145817976846No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8481.8384425269912847No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7801.691020248883493No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7651.6585006287126565No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6391.3853358192776308No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6291.3636560724970732No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6241.3528161991067944No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6231.3506482244287388No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5841.266097211984564No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC5111.1078350604864935No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5061.0969951870962147No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT4881.057971642891211No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT4811.0427958201448206No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4751.0297879720764862No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4731.0254520227203747No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4621.0016043012617613No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4500.9755886051250922No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4490.9734206304470364No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4260.923557212851754No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4150.8997094913931405No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4000.8671898712223042No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3170.687247972943676No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3100.6720721501972857No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3070.6655682261631184No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3050.6612322768070069No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3040.6590643021289511No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2990.6482244287386724No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2990.6482244287386724No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2930.6352165806703378No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2820.6113688592117245No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT2820.6113688592117245No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2820.6113688592117245No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2800.6070329098556129No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2650.5745132896847764No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2640.5723453150067207No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2600.5636734162944977No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2600.5636734162944977No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2550.5528335429042188No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC2350.5094740493431037No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2340.507306074665048No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2260.4899622772406019No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2200.47695442917226727No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2190.4747864544942115No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2180.47261847981615573No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2130.461778606425877No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1970.4270910115769848No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1930.41841911286476174No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1880.407579239474483No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1880.407579239474483No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1820.3945713914061484No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1790.3880674673719811No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1730.37505961930364656No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1700.36855569526947923No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1690.3663877205914235No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1680.36421974591336775No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1640.3555478472011447No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1600.34687594848892167No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1600.34687594848892167No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1590.3447079738108659No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1540.33386810042058707No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1530.3317001257425313No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1530.3317001257425313No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1480.3208602523522525No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1370.29701253089363916No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1340.2905086068594719No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1310.2840046828253046No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1300.28183670814724887No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1300.28183670814724887No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1300.28183670814724887No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1280.27750075879113734No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1270.27533278411308154No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC1260.2731648094350258No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1250.27099683475697006No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1240.26882886007891427No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1230.2666608854008585No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1220.26449291072280273No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1180.2558210120105797No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1180.2558210120105797No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1180.2558210120105797No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1150.24931708797641242No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1150.24931708797641242No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1140.24714911329835665No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1140.24714911329835665No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1120.24281316394224517No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1110.2406451892641894No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1100.23847721458613363No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1080.2341412652300221No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.2341412652300221No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.2341412652300221No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1060.2298053158739106No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC1030.2233013918397433No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1010.2189654424836318No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT980.2124615184494645No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA940.2037896197372415No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA920.19945367038112996No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT910.1972856957030742No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA910.1972856957030742No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT870.18861379699085115No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT850.18427784763473962No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC840.18210987295668388No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA830.1799418982786281No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT820.17777392360057234No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.17777392360057234No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC810.1756059489225166No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA810.1756059489225166No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC790.17126999956640507No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA780.1691020248883493No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC780.1691020248883493No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT780.1691020248883493No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA780.1691020248883493No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT780.1691020248883493No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG770.16693405021029353No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG770.16693405021029353No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT760.16476607553223777No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT750.16259810085418203No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT720.15609417682001475No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA690.14959025278584748No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT690.14959025278584748No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC660.14308632875168018No Hit
ATCAGATTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT660.14308632875168018No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA650.14091835407362444No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.14091835407362444No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.13875037939556867No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT640.13875037939556867No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA640.13875037939556867No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC640.13875037939556867No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT640.13875037939556867No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT640.13875037939556867No Hit
ATCAGATTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT630.1365824047175129No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA630.1365824047175129No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT620.13441443003945713No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT610.13224645536140137No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC610.13224645536140137No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA610.13224645536140137No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT610.13224645536140137No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC590.12791050600528986No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT580.1257425313272341No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC580.1257425313272341No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT570.12357455664917832No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT560.12140658197112258No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA560.12140658197112258No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCC560.12140658197112258No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA530.1149026579369553No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT530.1149026579369553No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA530.1149026579369553No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT520.11273468325889953No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT510.11056670858084376No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG500.10839873390278802No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT500.10839873390278802No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.10839873390278802No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA480.10406278454667649No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT480.10406278454667649No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA470.10189480986862075No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA470.10189480986862075No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC470.10189480986862075No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA470.10189480986862075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)