FastQCFastQC Report
Sun 21 May 2023
EGAF00007838126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41731
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT11912.8539934341376916No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA10282.4633965157796363No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9972.3891112122882268No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9312.230955404854904No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8872.1255181998993553No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8171.957777192015528No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7361.7636768828928135No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6741.615106275909995No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6311.5120653710670724No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6201.4857060698281852No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC6061.4521578682514198No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5761.3802688648726367No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5631.3491169634084972No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT5521.32275766216961No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5421.298794661043349No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5371.2868131604802184No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5361.2844168603675925No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA5271.2628501593539576No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5211.2484723586782007No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA5011.2005463564256789No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4681.1214684527090173No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4481.073542450456495No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4481.073542450456495No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4160.9968608468524598No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4130.9896719465145815No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4090.980086746064077No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT4040.9681052455009465No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3790.908197742685294No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3650.8746495411085284No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3570.8554791402075195No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3520.8434976396443891No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA3400.8147420382928758No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3350.8027605377297452No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA3260.7811938367161103No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3140.7524382353645971No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC3050.7308715343509621No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2860.6853418322110661No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC2760.661378831084805No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2710.6493973305216745No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2580.6182454290575351No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2550.6110565287196569No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2500.5990750281565262No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2450.5870935275933958No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2440.5846972274807697No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2350.5631305264671348No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2190.5247897246651171No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2170.5199971244398648No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC2140.5128082241019866No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2080.4984304234262299No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1990.47686372241259495No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1970.47207112218734276No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC1900.45529702139896No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1870.44810812106108167No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1860.4457118209484556No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1850.4433155208358294No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1850.4433155208358294No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1750.41935251970956844No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1720.41216361937169005No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC1710.409767319259064No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1700.4073710191464379No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1680.40257841892118573No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1650.39538951858330734No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1590.38101171790755073No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1530.3666339172317941No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1530.3666339172317941No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1500.35944501689391584No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1470.3522561165560375No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1460.34985981644341135No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC1350.3235005152045242No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1350.3235005152045242No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1330.31870791497927203No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1310.3139153147540198No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1290.30912271452876755No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1280.30672641441614146No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1270.30433011430351536No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.294744913853011No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1230.294744913853011No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1220.29234861374038484No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1200.28755601351513266No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1170.2803671131772543No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1160.27797081306462823No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1160.27797081306462823No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1090.26119671227624547No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1090.26119671227624547No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1090.26119671227624547No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1090.26119671227624547No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1070.2564041120509933No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1060.2540078119383672No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1030.24681891160048883No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1000.23963001126261052No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC970.23244111092473224No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA960.23004481081210612No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT950.22764851069948No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT940.2252522105868539No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT910.21806331024897557No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA890.21327071002372336No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA890.21327071002372336No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA870.20847810979847115No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT870.20847810979847115No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT840.20128920946059287No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT840.20128920946059287No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC830.19889290934796675No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC820.19649660923534065No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC820.19649660923534065No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT820.19649660923534065No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG810.19410030912271453No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA800.19170400901008844No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGATACCCGCACC790.18930770889746232No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.1869114087848362No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT780.1869114087848362No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT780.1869114087848362No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC770.1845151086722101No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC760.18211880855958398No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT750.17972250844695792No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACC750.17972250844695792No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG750.17972250844695792No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT720.17253360810907958No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA700.16774100788382737No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT690.16534470777120125No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.16055210754594906No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT640.15336320720807073No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA630.15096690709544464No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA620.14857060698281854No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA620.14857060698281854No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA620.14857060698281854No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA620.14857060698281854No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.14857060698281854No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC610.14617430687019242No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCAAGCGTTG610.14617430687019242No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT600.14377800675756633No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.14377800675756633No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA560.13419280630706187No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC550.1317965061944358No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT550.1317965061944358No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA550.1317965061944358No Hit
ATCAGATTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT540.1294002060818097No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT530.1270039059691836No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT530.1270039059691836No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT530.1270039059691836No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC530.1270039059691836No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT520.12460760585655747No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC520.12460760585655747No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC520.12460760585655747No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA520.12460760585655747No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTATTTGCTCCCCACGCT520.12460760585655747No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.12221130574393137No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA510.12221130574393137No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC510.12221130574393137No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA500.11981500563130526No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCAAGCGTTG500.11981500563130526No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA490.11741870551867917No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT490.11741870551867917No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACC470.11262610529342695No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA470.11262610529342695No Hit
ATCAGATTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT460.11022980518080085No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA460.11022980518080085No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT450.10783350506817474No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT440.10543720495554863No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA440.10543720495554863No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA440.10543720495554863No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT440.10543720495554863No Hit
ATCAGATTGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT430.10304090484292251No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA430.10304090484292251No Hit
ATCAGATTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT430.10304090484292251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)