FastQCFastQC Report
Sun 21 May 2023
EGAF00007838137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75510
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA66548.812077870480731No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA64418.529996027016288No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT61268.112832737385776No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA48356.40312541385247No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA45125.975367500993245No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT27843.686928883591577No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT21582.857899615944908No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT19852.628790888624023No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT17062.259303403522712No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11911.577274533174414No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10991.4554363660442324No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT10571.3998145940934976No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT8531.129651701761356No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8221.0885975367500995No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7360.9747053370414516No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7200.9535160905840285No Hit
ATCAGATTGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT6360.8422725466825586No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6010.7959210700569461No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5960.7892994305390014No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT5930.7853264468282347No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5620.7442722818169779No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5390.7138127400344325No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5270.6979208051913653No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5060.6701099192159979No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4940.6542179843729308No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4830.6396503774334525No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4570.6052178519401404No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4420.5853529333863065No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4310.5707853264468282No Hit
ATCAGATTGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3900.5164878823996821No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3860.5111905707853265No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3830.5072175870745597No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3800.5032446033637928No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3500.463514766256125No Hit
ATCAGATTGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT3380.44762283141305786No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3370.4462985035094689No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3140.4158389617269236No Hit
ATCAGATTGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3100.4105416501125679No Hit
ATCAGATTGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2810.37213614090848895No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2790.3694874851013111No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2670.35359555025824396No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2520.33373063170441003No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2410.3191630247649318No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2360.31254138524698716No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2300.3045954178254536No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2290.3032710899218647No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1990.26354125281419677No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1980.2622169249106079No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1980.2622169249106079No Hit
ATCAGATTGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1970.26089259700701894No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1960.25956826910343No Hit
ATCAGATTGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1950.25824394119984106No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1920.2542709574890743No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1840.2436763342603629No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1780.23573036683882928No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1730.22910872732088464No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1710.22646007151370678No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1660.21983843199576214No Hit
ATCAGATTGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1650.21851410409217323No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1630.21586544828499535No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1510.1999735134419282No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1510.1999735134419282No Hit
ATCAGATTGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1500.1986491855383393No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1440.1907032181168057No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1420.1880545623096279No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1390.18408157859886107No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1370.18143292279168322No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1330.1761356111773275No Hit
ATCAGATTGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1310.17348695537014966No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1300.17216262746656072No Hit
ATCAGATTGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1170.15494636471990467No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1160.1536220368163157No Hit
ATCAGATTGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1160.1536220368163157No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1150.1522977089127268No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1130.14964905310554893No Hit
ATCAGATTGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1120.14832472520196002No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1120.14832472520196002No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1110.14700039729837108No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1090.1443517414911932No Hit
ATCAGATTGCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1090.1443517414911932No Hit
ATCAGATTGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1090.1443517414911932No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1070.14170308568401535No Hit
ATCAGATTGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1070.14170308568401535No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1060.14037875778042644No Hit
ATCAGATTGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1010.1337571182624818No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA980.129784134551715No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA970.12845980664812606No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG970.12845980664812606No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT970.12845980664812606No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA940.1244868229373593No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT900.11918951132300357No Hit
ATCAGATTGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT880.11654085551582571No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA850.11256787180505892No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG840.11124354390147No Hit
ATCAGATTGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT840.11124354390147No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA840.11124354390147No Hit
ATCAGATTGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC830.10991921599788107No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA830.10991921599788107No Hit
ATCAGATTGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT790.10462190438352537No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT780.10329757647993643No Hit
ATCAGATTGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC770.1019732485763475No Hit
ATCAGATTGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT770.1019732485763475No Hit
ATCAGATTGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT770.1019732485763475No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA760.10064892067275857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)