FastQCFastQC Report
Sun 21 May 2023
EGAF00007838162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35133
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA18465.254319300942134No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT11323.2220419548572568No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11153.1736543989980928No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT10863.09111092135599No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8442.402299832066718No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8382.3852218711752484No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7452.1205134773574703No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7252.0635869410525713No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7072.0123530583781633No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6511.852958756724447No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5751.6366379187658326No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5341.5199385193407906No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4761.354851564056585No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4501.280847066860217No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4301.2239205305553182No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4271.2153815501095835No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4261.2125352232943385No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3961.127145418836991No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3731.0616799020863577No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3621.0303703071186634No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3541.007599692596704No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3450.9819827512594995No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3370.9592121367375401No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3300.9392878490308257No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3270.930748868585091No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2850.8112031423448041No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2800.7969715082685794No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2800.7969715082685794No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2710.771354566931375No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2650.7542766060399055No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2610.7428912987789258No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2490.7087353769959867No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2470.7030427233654968No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2410.6859647624740273No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2310.657501494321578No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2280.6489625138758432No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2240.6375772066148635No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2220.6318845529843736No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2170.617652918908149No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2030.5778043434947201No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2020.5749580166794751No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1910.543648421711781No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1760.5009535194831071No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1710.4867218854068824No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1620.4611049440696781No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1600.45541229043918824No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1600.45541229043918824No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1600.45541229043918824No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1570.4468733099934534No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1550.4411806563629636No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1530.43548800273247373No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1500.4269490222867389No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1490.424102695471494No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1340.38140779324282015No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1270.3614835055361057No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1260.35863717872086076No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1240.3529445250903709No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1230.35009819827512595No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1220.347251871459881No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.3358665641989013No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1180.3358665641989013No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1160.3301739105684115No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1140.3244812569379216No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1140.3244812569379216No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1120.31878860330743175No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1110.3159422764921868No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1090.310249622861697No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1050.2988643156007173No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1030.2931716619702274No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1030.2931716619702274No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT980.2789400278940028No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA940.2675547206330231No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA930.26470839381777816No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA920.2618620670025332No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT900.25616941337204335No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA880.25047675974155353No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT850.24193777929581872No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC850.24193777929581872No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA850.24193777929581872No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA830.2362451256653289No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA800.22770614521959412No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT800.22770614521959412No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA790.22485981840434918No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA790.22485981840434918No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA790.22485981840434918No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT780.22201349158910427No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT750.21347451114336946No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT750.21347451114336946No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT730.20778185751287961No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA720.20493553069763473No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT720.20493553069763473No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA710.2020892038823898No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA710.2020892038823898No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT690.19639655025189992No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA690.19639655025189992No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA690.19639655025189992No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA670.19070389662141007No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA670.19070389662141007No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA670.19070389662141007No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG660.18785756980616514No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC660.18785756980616514No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA650.1850112429909202No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC650.1850112429909202No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT630.17931858936043038No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT620.17647226254518544No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.17647226254518544No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT590.16793328209945066No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.16508695528420575No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT570.1622406284689608No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT560.15939430165371588No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT550.15654797483847094No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT540.15370164802322603No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT540.15370164802322603No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC540.15370164802322603No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA520.14800899439273618No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.14516266757749124No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT510.14516266757749124No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG510.14516266757749124No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA500.1423163407622463No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA500.1423163407622463No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG500.1423163407622463No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.1423163407622463No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA490.1394700139470014No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA490.1394700139470014No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA490.1394700139470014No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT490.1394700139470014No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT480.13662368713175646No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA470.13377736031651155No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA470.13377736031651155No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA460.1309310335012666No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT460.1309310335012666No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA450.12808470668602168No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT450.12808470668602168No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT450.12808470668602168No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC440.12523837987077677No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC440.12523837987077677No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT430.12239205305553183No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG430.12239205305553183No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC400.11385307260979706No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT400.11385307260979706No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT390.11100674579455214No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT390.11100674579455214No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT390.11100674579455214No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT380.1081604189793072No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC380.1081604189793072No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC380.1081604189793072No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCGCACC380.1081604189793072No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA370.10531409216406228No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT370.10531409216406228No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT370.10531409216406228No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT360.10246776534881737No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT360.10246776534881737No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT360.10246776534881737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)