FastQCFastQC Report
Sun 21 May 2023
EGAF00007838163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35133
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA15454.397574929553412No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT13093.7258418011556085No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT12183.466826060968321No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11023.1366521503999087No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8732.484843309708821No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8642.4592263683716165No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7792.217288589075798No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6491.8472661030939572No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5791.6480232260268124No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5681.6167136310591184No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5271.500014231634076No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4741.349158910426095No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4321.2296131841858082No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4021.1442233797284604No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3901.1100674579455212No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3861.0986821506845414No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3841.0929894970540517No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3771.0730652093473374No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3561.0132923462271939No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3360.9563658099222951No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3320.9449805026613156No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3240.9222098881393562No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3130.890900293171662No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2880.8197421227905389No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2870.8168957959752939No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2860.814049469160049No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2840.8083568155295592No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2780.7912788546380896No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2710.771354566931375No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2650.7542766060399055No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2570.731505991517946No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2450.697350069735007No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2420.6888110892892721No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2340.6660404747673129No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2290.6518088406910881No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2290.6518088406910881No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2280.6489625138758432No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2270.6461161870605983No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2230.6347308797996186No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2220.6318845529843736No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2040.580650670309965No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2000.5692653630489852No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1950.5550337289727606No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1890.5379557680812911No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1840.5237241340050665No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1690.4810292317763925No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1540.43833432954771867No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1530.43548800273247373No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1530.43548800273247373No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1500.4269490222867389No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1480.4212563686562491No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1470.41841004184100417No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1440.40987106139526946No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1390.3956394273190448No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1380.39279310050379984No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1340.38140779324282015No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1330.3785614664275752No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1320.3757151396123303No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1310.37286881279708534No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1270.3614835055361057No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1260.35863717872086076No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1200.34155921782939114No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1180.3358665641989013No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1170.33302023738365644No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.3301739105684115No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1150.32732758375316656No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1130.3216349301226767No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1110.3159422764921868No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1100.3130959496769419No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1090.310249622861697No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1090.310249622861697No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1050.2988643156007173No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1050.2988643156007173No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1020.2903253351549825No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1020.2903253351549825No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1000.2846326815244926No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1000.2846326815244926No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA970.27609370107875786No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA940.2675547206330231No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA890.25332308655679847No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA880.25047675974155353No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA870.2476304329263086No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA850.24193777929581872No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT840.23909145248057379No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA830.2362451256653289No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA810.23055247203483906No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT790.22485981840434918No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC740.21062818432812455No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT740.21062818432812455No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT700.19924287706714486No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA700.19924287706714486No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA670.19070389662141007No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC670.19070389662141007No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT670.19070389662141007No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA660.18785756980616514No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA660.18785756980616514No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA650.1850112429909202No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA640.1821649161756753No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT630.17931858936043038No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA610.1736259357299405No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA610.1736259357299405No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT600.17077960891469557No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA600.17077960891469557No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT600.17077960891469557No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC590.16793328209945066No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.16793328209945066No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT590.16793328209945066No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT580.16508695528420575No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT570.1622406284689608No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA560.15939430165371588No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA560.15939430165371588No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT560.15939430165371588No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT560.15939430165371588No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG560.15939430165371588No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT560.15939430165371588No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA540.15370164802322603No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC540.15370164802322603No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG530.15085532120798112No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.15085532120798112No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA520.14800899439273618No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.14800899439273618No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.14800899439273618No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG510.14516266757749124No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC510.14516266757749124No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.1423163407622463No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA490.1394700139470014No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA490.1394700139470014No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA490.1394700139470014No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT480.13662368713175646No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT480.13662368713175646No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA480.13662368713175646No Hit
ATCCTACATCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT480.13662368713175646No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT470.13377736031651155No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA470.13377736031651155No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA460.1309310335012666No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT450.12808470668602168No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT440.12523837987077677No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT430.12239205305553183No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG420.11954572624028689No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT420.11954572624028689No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT420.11954572624028689No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT410.116699399425042No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT410.116699399425042No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT410.116699399425042No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT390.11100674579455214No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG390.11100674579455214No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT380.1081604189793072No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT370.10531409216406228No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC370.10531409216406228No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC370.10531409216406228No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA360.10246776534881737No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT360.10246776534881737No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT360.10246776534881737No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC360.10246776534881737No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA360.10246776534881737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)