FastQCFastQC Report
Sun 21 May 2023
EGAF00007838180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47940
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA21664.518147684605757No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA19314.027951606174385No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT15033.1351689612015017No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG11552.409261576971214No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11432.384230287859825No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT10152.117229870671673No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9401.9607843137254901No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8941.8648310387984983No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8571.7876512307050478No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA8261.7229870671672924No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6901.4392991239048811No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6581.3725490196078431No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA6431.3412599082186065No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6151.2828535669586982No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5901.2307050479766375No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5711.1910721735502712No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5701.1889862327909888No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT5421.1305798915310805No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5261.0972048393825615No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5031.0492282019190655No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4901.0221109720483939No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4500.9386733416770965No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4140.8635794743429287No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3970.8281184814351272No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3860.8051731330830205No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3850.803087192323738No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3830.7989153108051731No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3730.7780559032123487No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3650.7613683771380892No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3630.7571964956195244No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3630.7571964956195244No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3500.7300792657488527No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT3490.7279933249895703No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3450.7196495619524406No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3420.7133917396745932No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3400.7092198581560284No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3400.7092198581560284No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3370.7029620358781811No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3310.6904463913224864No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3240.6758448060075094No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3060.6382978723404255No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2940.6132665832290363No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2800.5840634125990822No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2260.4714226115978306No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2260.4714226115978306No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2240.46725073007926576No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2190.45682102628285354No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2150.44847726324572385No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2080.4338756779307468No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2060.42970379641218187No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2050.4276178556528994No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2050.4276178556528994No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1950.4067584480600751No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1920.4005006257822278No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1900.3963287442636629No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1890.3942428035043805No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1880.39215686274509803No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1850.38589904046725076No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1830.38172715894868586No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1800.37546933667083854No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1790.3733833959115561No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1770.36921151439299127No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1760.3671255736337088No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1720.35878181059657904No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1690.3525239883187317No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1650.344180225281602No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1590.3316645807259074No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1590.3316645807259074No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1550.32332081768877763No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1510.3149770546516479No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1500.31289111389236546No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1490.31080517313308303No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1480.3087192323738006No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1430.2982895285773884No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1390.28994576554025864No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT1350.2816020025031289No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1340.27951606174384647No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1280.2670004171881519No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1280.2670004171881519No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.2461410095953275No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1170.24405506883604508No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT1160.2419691280767626No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1150.23988318731748018No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1130.2357113057989153No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1120.23362536503963288No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1120.23362536503963288No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1110.23153942428035043No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1100.229453483521068No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.22528160200250313No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1070.22319566124322068No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1060.22110972048393823No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCC1050.2190237797246558No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1020.2127659574468085No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1010.2106800166875261No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT990.20650813516896122No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC990.20650813516896122No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA980.20442219440967874No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA970.2023362536503963No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG950.19816437213183144No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA940.19607843137254902No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA930.1939924906132666No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA930.1939924906132666No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT900.18773466833541927No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA890.18564872757613685No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT890.18564872757613685No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA880.1835627868168544No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA860.17939090529828952No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC850.1773049645390071No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA850.1773049645390071No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC850.1773049645390071No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT830.17313308302044222No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA830.17313308302044222No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT820.1710471422611598No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.1710471422611598No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT820.1710471422611598No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA800.1668752607425949No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.1668752607425949No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT800.1668752607425949No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT800.1668752607425949No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT790.16478931998331248No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT770.1606174384647476No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA770.1606174384647476No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT770.1606174384647476No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC760.15853149770546515No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT750.15644555694618273No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC750.15644555694618273No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT750.15644555694618273No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA740.1543596161869003No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA730.15227367542761786No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA730.15227367542761786No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT720.1501877346683354No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT700.14601585314977056No Hit
TGAGGTTGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG690.1439299123904881No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT680.14184397163120568No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT680.14184397163120568No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA650.13558614935335836No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC650.13558614935335836No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT630.13141426783479349No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA620.12932832707551106No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCC620.12932832707551106No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC610.1272423863162286No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT600.1251564455569462No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.12307050479766375No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT580.1209845640383813No Hit
ATCCTACATCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT570.11889862327909886No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT560.11681268251981644No Hit
TGAGGTTGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG560.11681268251981644No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT550.114726741760534No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT540.11264080100125157No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT540.11264080100125157No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC540.11264080100125157No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT520.1084689194826867No Hit
TGAGGTTGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA500.10429703796412182No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT500.10429703796412182No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT500.10429703796412182No Hit
TGAGGTTGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA500.10429703796412182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)