FastQCFastQC Report
Sun 21 May 2023
EGAF00007838188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62184
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA32545.23285732664351No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA29894.806702688794545No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT22173.5652257815515247No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT16162.5987392255242505No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT14892.3945066254985203No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13842.225652901067799No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA13002.090569921523221No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA12321.9812170333204684No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA12011.931364981345684No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10251.6483339765856169No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9241.4859127749903511No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT8911.4328444615978386No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8691.3974655860028302No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8571.3781680174964621No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7901.2704232600025729No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT7731.2430850379518847No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT7421.1932329859771003No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA6781.0903126206098033No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6581.0581500064325229No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6241.0034735623311464No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT5880.9455808568120416No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5840.9391483339765857No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5830.9375402032677217No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5780.9294995497234014No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5770.9278914190145374No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5700.9166345040524894No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5340.8587417985333847No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5070.8153222693940563No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4860.7815515245079121No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4830.77672713238132No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4420.7107937733178953No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4390.7059693811913033No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4210.6770230284317509No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4120.6625498520519748No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA4100.6593335906342468No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4010.6448604142544706No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3770.6062652772417342No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3710.5966164929885501No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3640.585359578026502No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3600.5789270551910459No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT3590.5773189244821819No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA3500.5628457481024057No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3410.5483725717226295No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3350.5387237874694455No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3210.5162099575453493No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3090.4969123890389811No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3060.492087996912389No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3010.484047343368069No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3000.482439212659205No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2810.4518847291907886No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2770.44545220635533256No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2720.43741155281101246No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2700.4341952913932845No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2670.42937089926669236No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2660.42776276855782835No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC2620.42133024572237227No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2590.41650585359578024No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2470.3972082850894121No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2470.3972082850894121No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2450.393992023671684No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2430.39077576225395605No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2430.39077576225395605No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2360.3795188472919079No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2320.3730863244564518No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2300.3698700630387238No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT2230.3586131480766757No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2130.3425318409880355No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2130.3425318409880355No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC2110.33931557957030745No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2070.3328830567348514No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2050.3296667953171234No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2000.3216261417728033No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1900.30554483468416316No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1780.28624726617779495No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1780.28624726617779495No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1770.2846391354689309No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1740.27981474334233886No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1730.27820661263347485No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1710.27499035121574683No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1620.26051717483597064No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1610.25890904412710664No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1560.2508683905827866No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1530.2460439984561945No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1460.23478708349414643No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1450.2331789527852824No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1450.2331789527852824No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1380.22192203782323427No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1370.22031390711437027No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1370.22031390711437027No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1320.21227325357005017No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT1300.20905699215232215No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1290.20744886144345812No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1220.19619194648141003No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1190.19136755435481798No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.18975942364595394No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1170.18815129293708993No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1140.18332690081049788No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1140.18332690081049788No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1140.18332690081049788No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1130.18171877010163387No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1130.18171877010163387No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1120.18011063939276983No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1080.17367811655731377No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1070.17206998584844976No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC1040.1672455937218577No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1040.1672455937218577No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1030.1656374630129937No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1030.1656374630129937No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1020.16402933230412967No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1010.16242120159526566No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT990.15920494017753764No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC980.1575968094686736No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT970.1559886787598096No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA950.15277241734208158No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA950.15277241734208158No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA950.15277241734208158No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC950.15277241734208158No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT920.1479480252154895No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA870.13990737167116943No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT870.13990737167116943No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC870.13990737167116943No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA860.13829924096230542No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT860.13829924096230542No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT850.1366911102534414No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA840.1350829795445774No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC840.1350829795445774No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT810.13025858741798532No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA800.1286504567091213No Hit
ATCCTACATCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT800.1286504567091213No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA800.1286504567091213No Hit
TGGTACAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA790.1270423260002573No Hit
TGGTACAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA770.12382606458252927No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT750.12060980316480124No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT730.11739354174707321No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT730.11739354174707321No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT720.11578541103820918No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT720.11578541103820918No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC710.11417728032934515No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.11256914962048115No Hit
TGGTACAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA700.11256914962048115No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC700.11256914962048115No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT690.11096101891161714No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT690.11096101891161714No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC690.11096101891161714No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT680.10935288820275312No Hit
TGGTACAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA670.1077447574938891No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT660.10613662678502508No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT650.10452849607616108No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC650.10452849607616108No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC640.10292036536729705No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT630.10131223465843305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)