FastQCFastQC Report
Sun 21 May 2023
EGAF00007838198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007838198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49999
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14522.9040580811616232No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT14152.8300566011320227No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14122.824056481129623No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG13762.752055041100822No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG12772.5540510810216204No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT11542.3080461609232183No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9131.8260365207304146No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9021.8040360807216143No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8971.7940358807176142No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8681.736034720694414No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA8201.640032800656013No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7701.5400308006160122No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7361.472029440588812No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7331.4660293205864117No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT7241.4480289605792116No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7201.4400288005760116No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG6791.3580271605432108No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6691.3380267605352107No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG6551.3100262005240104No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT5241.0480209604192083No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5011.002020040400808No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4770.9540190803816077No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4730.9460189203784075No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4680.9360187203744075No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4600.9200184003680074No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT4510.9020180403608071No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4230.8460169203384068No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4180.8360167203344068No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4170.8340166803336068No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3980.7960159203184063No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3930.7860157203144064No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3900.7800156003120062No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3630.7260145202904058No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC3550.7100142002840056No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA3540.7080141602832056No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3530.7060141202824057No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3440.6880137602752056No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA3440.6880137602752056No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3410.6820136402728055No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3310.6620132402648052No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3020.6040120802416048No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2930.5860117202344046No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2900.5800116002320046No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2820.5640112802256045No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2790.5580111602232045No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2700.5400108002160042No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2610.5220104402088042No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2540.5080101602032041No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2530.5060101202024041No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT2520.504010080201604No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2490.49800996019920396No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2470.494009880197604No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2320.4640092801856037No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT2210.44200884017680353No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2070.4140082801656033No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2070.4140082801656033No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2050.41000820016400324No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1960.3920078401568031No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1960.3920078401568031No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1940.3880077601552031No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1930.3860077201544031No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1880.376007520150403No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1840.368007360147203No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1840.368007360147203No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1820.3640072801456029No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1800.3600072001440029No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1780.3560071201424029No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1750.3500070001400028No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1740.3480069601392028No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1730.3460069201384028No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1700.3400068001360027No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1680.3360067201344027No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1680.3360067201344027No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1660.3320066401328026No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1630.3260065201304026No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1620.3240064801296026No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1560.3120062401248025No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1560.3120062401248025No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT1530.30600612012240247No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1520.3040060801216024No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT1510.3020060401208024No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1420.2840056801136023No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1410.28200564011280227No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1370.2740054801096022No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1360.2720054401088022No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1320.2640052801056021No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.252005040100802No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1260.252005040100802No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1240.24800496009920198No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1240.24800496009920198No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC1220.24400488009760196No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1170.23400468009360187No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1140.22800456009120182No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1140.22800456009120182No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1130.22600452009040184No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1100.2200044000880018No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1090.21800436008720175No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1090.21800436008720175No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1070.21400428008560168No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1060.2120042400848017No Hit
ATCCTACATCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1060.2120042400848017No Hit
ATCCTACATCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1040.20800416008320163No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1040.20800416008320163No Hit
ATCCTACATCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT1010.20200404008080158No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1000.2000040000800016No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA980.19600392007840156No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA970.19400388007760155No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC940.1880037600752015No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA930.1860037200744015No Hit
ATCCTACATCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.1860037200744015No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC930.1860037200744015No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT920.1840036800736015No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA920.1840036800736015No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC910.18200364007280145No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA880.1760035200704014No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA870.1740034800696014No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT870.1740034800696014No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT870.1740034800696014No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCATGCT860.1720034400688014No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA860.1720034400688014No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT850.17000340006800135No Hit
ATCCTACATCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT840.16800336006720135No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA810.1620032400648013No Hit
ATCCTACATCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT800.16000320006400126No Hit
ATCCTACATCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC800.16000320006400126No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA790.15800316006320125No Hit
ATCCTACATCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT790.15800316006320125No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT790.15800316006320125No Hit
ATCCTACATCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT770.15400308006160124No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT730.14600292005840118No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT730.14600292005840118No Hit
ATTCATAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA720.14400288005760117No Hit
ATCCTACATCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC720.14400288005760117No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT720.14400288005760117No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT700.14000280005600113No Hit
ATCCTACATCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT700.14000280005600113No Hit
ATCCTACATCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT690.1380027600552011No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA690.1380027600552011No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT670.13400268005360108No Hit
ATCCTACATCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT640.12800256005120103No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGGGGATACGAGCGTTA640.12800256005120103No Hit
ATCCTACATCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT630.126002520050401No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA620.12400248004960099No Hit
ATCCTACATCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.12200244004880098No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA610.12200244004880098No Hit
ATTCATAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA590.11800236004720094No Hit
ATCCTACATCCGGACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCT580.11600232004640093No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA570.11400228004560091No Hit
ATCCTACATCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.1120022400448009No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC560.1120022400448009No Hit
ATCCTACATCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC560.1120022400448009No Hit
ATCCTACATCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT550.1100022000440009No Hit
ATCCTACATCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC540.10800216004320086No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA530.10600212004240085No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAATACGGGGGATACGAGCGTTA520.10400208004160082No Hit
ATCCTACATCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.10400208004160082No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG510.10200204004080081No Hit
ATTCATAGGGTGTGCCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG510.10200204004080081No Hit
ATTCATAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA500.1000020000400008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)